pAAVLINK-EF1a-HA-5'-SETD2

LOCUS       pAAVLINK-EF1a-HA-5'-SETD2        7267 bp DNA     circular SYN 20-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        146..688
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..4407
                     /codon_start=1
                     /label=SETD2
                     /translation="KQLQPQPPPKMGDFYDPEHPTPEEEENEAKIENVQKTGFIKGPMF
                     KGVASSRFLPKGTKTKVNLEEQGRQKVSFSFSLTKKTLQNRFLTALGNEKQSDTPNPPA
                     VPLQVDSTPKMKMEIGDTLSTAEESSPPKSRVELGKIHFKKHLLHVTSRPLLATTTAVA
                     SPPTHAAPLPAVIAESTTVDSPPSSPPPPPPPAQATTLSSPAPVTEPVALPHTPITVLM
                     AAPVPLPVDVAVRSLKEPPIIIVPESLEADTKQDTISNSLEEHVTQILNEQADISSKKE
                     DSHIGKDEEIPDSSKISLSCKKTGSKKKSSQSEGIFLGSESDEDSVRTSSSQRSHDLKF
                     SASIEKERDFKKSSAPLKSEDLGKPSRSKTDRDDKYFSYSKLERDTRYVSSRCRSERER
                     RRSRSHSRSERGSRTNLSYSRSERSHYYDSDRRYHRSSPYRERTRYSRPYTDNRARESS
                     DSEEEYKKTYSRRTSSHSSSYRDLRTSSYSKSDRDCKTETSYLEMERRGKYSSKLERES
                     KRTSENEAIKRCCSPPNELGFRRGSSYSKHDSSASRYKSTLSKPIPKSDKFKNSFCCTE
                     LNEEIKQSHSFSLQTPCSKGSELRMINKNPEREKAGSPAPSNRLNDSPTLKKLDELPIF
                     KSEFITHDSHDSIKELDSLSKVKNDQLRSFCPIELNINGSPGAESDLATFCTSKTDAVL
                     MTSDDSVTGSELSPLVKACMLSSNGFQNISRCKEKDLDDTCMLHKKSESPFRETEPLVS
                     PHQDKLMSMPVMTVDYSKTVVKEPVDTRVSCCKTKDSDIYCTLNDSNPSLCNSEAENIE
                     PSVMKISSNSFMNVHLESKPVICDSRNLTDHSKFACEEYKQSIGSTSSASVNHFDDLYQ
                     PIGSSGIASSLQSLPPGIKVDSLTLLKCGENTSPVLDAVLKSKKSSEFLKHAGKETIVE
                     VGSDLPDSGKGFASRENRRNNGLSGKCLQEAQEEGNSILPERRGRPEISLDERGEGGHV
                     HTSDDSEVVFSSCDLNLTMEDSDGVTYALKCDSSGHAPEIVSTVHEDYSGSSESSNDES
                     DSEDTDSDDSSIPRNRLQSVVVVPKNSTLPMEETSPCSSRSSQSYRHYSDHWEDERLES
                     RRHLYEEKFESIASKACPQTDKFFLHKGTEKNPEISFTQSSRKQIDNRLPELSHPQSDG
                     VDSTSHTDVKSDPLGHPNSEETVKAKIPSRQQEELPIYSSDFEDVPNKSWQQTTFQN"
     misc_feature    3691..3696
                     /label=SDS
     intron          4408..4448
                     /label=Splicing Donor
     misc_feature    4452..4485
                     /label=loxP-JT1571
     repeat_region   4530..4670
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4530..4659
                     /label=AAV2 ITR
     rep_origin      4745..5200
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5482..5586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5587..6447
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6618..7206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctaagc agctgcagcc gcagccgcct ccgaagatgg gggatttcta
      781 cgacccggag cacccgaccc ctgaagaaga agaaaatgag gcaaagattg aaaatgtgca
      841 gaaaacaggt ttcatcaaag gaccaatgtt caaaggtgtt gcttctagtc gatttttgcc
      901 caaaggcacc aaaacaaaag ttaatttgga agaacaggga cgacagaagg tgtcattcag
      961 cttcagcctt acaaagaaaa ctttgcagaa taggtttctc actgcacttg gcaatgaaaa
     1021 gcaaagtgat actccaaacc ctccagctgt acctcttcag gtagactcga ctcctaaaat
     1081 gaaaatggaa attggtgata ccttatctac tgcagaagaa tcttccccac caaagtcaag
     1141 ggtggaattg ggcaaaattc attttaagaa acatctgctt catgtaacat ccaggccact
     1201 gctggctact accacagcag tagcatctcc acctactcat gcagcaccat taccagcagt
     1261 gatagcagaa tcaacaactg tagactcacc gccctcatct ccgcctccac cgcctccacc
     1321 tgcccaagcc acaacactct catcaccagc accagtaaca gagccagtgg ccttgccaca
     1381 tacaccaata acagttctaa tggcagcacc agtaccctta ccagtagatg tagcagttag
     1441 atctctgaaa gaaccaccaa ttataattgt accagaatct ttagaagcag atactaagca
     1501 ggacactata tctaatagtt tagaagaaca cgtaactcaa atattgaatg agcaagcaga
     1561 tatttcctca aaaaaagaag attcccatat tgggaaggat gaagaaattc cagatagttc
     1621 taagattagt ctgagctgta aaaaaacagg ttctaagaag aaatcctcac aatctgaagg
     1681 catctttctt ggttcagaat ctgatgaaga ttctgtacgg acttcttcaa gtcaaagatc
     1741 acatgattta aaattttcag caagcattga aaaggaaaga gattttaaaa agagctcagc
     1801 acctttaaaa agtgaggatc tagggaaacc ttcacgatct aaaacagaca gagatgataa
     1861 atattttagc tattcaaaac ttgaaagaga tactcggtat gtatcttccc gatgtagatc
     1921 agaaagagag cgacggcgga gcagatctca ctctaggtct gagagaggct ctagaactaa
     1981 tttatcctat tccaggtcag aacgatctca ttattatgac tctgatcgtc gctaccatag
     2041 gagctcccct tatcgagaga ggacgcgcta ttctcggcca tacacagata acagagcacg
     2101 agagagttct gactcagaag aagagtataa gaagacatac tcaaggcgta cctcatctca
     2161 ttcctcttct tacagagacc taaggacatc atcctattct aaatctgatc gggactgtaa
     2221 aactgagacc tcttacttag agatggaaag aagaggcaag tattcttcaa aactagaaag
     2281 agaatctaaa aggacttcag aaaatgaagc aattaaaaga tgttgttctc cccctaatga
     2341 actgggattc cgacgagggt catcatattc taagcatgac agtagtgctt cccgttataa
     2401 atctaccctt tcaaaaccta tacccaagtc tgataaattt aaaaattctt tctgttgtac
     2461 agaattaaat gaagaaatca aacagtctca ttcttttagt ttacagacac cttgttcaaa
     2521 aggtagtgaa ttaagaatga ttaataaaaa tcctgaaaga gaaaaggctg ggtctccagc
     2581 tccatcaaat cgattaaatg attcacctac tttaaaaaag ctagatgaat tgcctatttt
     2641 taagtccgaa tttataacac atgatagcca tgatagtatt aaggaattag actctttatc
     2701 taaagtgaag aatgatcaat taagaagttt ttgtcccata gaattaaata taaatggatc
     2761 tcctggggca gaatctgatt tggcaacatt ttgcacttct aaaactgatg ctgttttaat
     2821 gacttctgat gatagtgtga ctggatcgga attatcccct ttggtcaaag catgcatgct
     2881 ttcatcaaat ggatttcaga atattagtag gtgcaaagaa aaagacttgg atgatacctg
     2941 catgctgcat aagaagtcag aaagcccatt tagagaaaca gaacctctgg tgtcaccaca
     3001 ccaagataaa ctcatgtcta tgccagttat gactgtggat tattccaaaa cagtagttaa
     3061 agaaccagtt gatacgaggg tttcttgctg caaaaccaaa gattcagaca tatactgtac
     3121 tttgaacgat agcaaccctt ctttgtgtaa ctctgaagct gaaaatattg agccttcagt
     3181 tatgaagatt tcttcaaata gctttatgaa tgtgcatttg gaatcaaaac cagttatatg
     3241 tgatagtaga aatttgacag atcactcaaa atttgcatgt gaagaatata agcagagcat
     3301 cggtagcact agttcagctt ctgttaatca ttttgatgat ttatatcaac ctattgggag
     3361 ttcaggtatt gcttcatctc ttcagagtct tccaccagga ataaaggtgg acagtctaac
     3421 tctcttgaaa tgcggagaga acacatctcc agttctggat gcagtgctaa agagtaaaaa
     3481 aagttcagag tttttaaagc atgcagggaa agaaacaata gtagaagtag gtagtgacct
     3541 tcctgattca ggaaagggat ttgcttccag ggagaacagg cgtaataatg ggttatctgg
     3601 gaaatgtttg caagaggctc aagaagaagg gaattccata ttgcctgaaa gaagaggaag
     3661 accagaaatc tctttagatg aaagaggaga aggaggacat gtgcatactt ctgatgactc
     3721 agaagttgta ttttcttctt gtgatttgaa tttaaccatg gaagacagtg atggtgtaac
     3781 ttatgcatta aagtgtgaca gtagtggtca tgccccagaa attgtgtcta cagttcatga
     3841 agattattct ggctcttctg aaagttcaaa tgatgaaagt gattcagaag atacagattc
     3901 ggatgatagc agtattccaa gaaaccgtct ccagtctgtt gtggttgtgc caaagaattc
     3961 tactttgccc atggaagaaa caagtccttg ttcttctcgg agcagtcaaa gttatagaca
     4021 ctattctgac cattgggaag atgagagatt ggagtcaagg agacatttgt atgaggaaaa
     4081 atttgaaagt atagcaagta aagcctgtcc tcaaactgat aagtttttcc ttcataaagg
     4141 aacagagaag aatccggaaa tttcttttac acagtccagt agaaaacaaa tagataaccg
     4201 cctgcctgaa ctttctcatc ctcagagtga tggggttgat agtacaagtc atacagatgt
     4261 gaaatctgac cctctgggtc acccaaattc agaggaaacc gtgaaagcca aaataccttc
     4321 taggcagcaa gaagagctgc caatttattc ttctgatttt gaagatgtcc caaataagtc
     4381 ttggcaacag accactttcc aaaacaggta agtatcaagg ttacaagaca ggtttaagga
     4441 gaccaataag ctattattcg tatagcatac attatacgaa gttattaagt tatggagacc
     4501 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4561 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4621 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4681 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4741 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4801 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     4861 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4921 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4981 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5041 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     5101 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     5161 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     5221 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5281 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5341 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5401 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5461 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5521 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5581 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5641 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5701 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5761 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5821 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5881 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5941 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6001 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6061 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6121 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6181 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6241 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6301 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6361 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6421 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6481 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6541 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6601 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6661 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6721 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6781 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6841 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6901 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6961 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7021 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7081 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7141 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7201 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7261 cacatgt
//