| 质粒名称: | pTre3G-mEGFP-iPAK4-CMV-rtTA3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000480 |
| 产品用途: | Fluorescent Tickertape Expression |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV, TRE3G |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mEGFP-iPAK4, rtTA3 |
| 物种来源: |
synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pTre3G-mEGFP-iPAK4-CMV-rtTA3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pTre3G-mEGFP-iPAK4-CMV-rtTA3载体质粒基本信息
pTre3G-mEGFP-iPAK4-CMV-rtTA3质粒图谱载体图谱和pTre3G-mEGFP-iPAK4-CMV-rtTA3载体序列质粒序列多克隆位点信息
pTre3G-mEGFP-iPAK4-CMV-rtTA3质粒载体简介
pTre3G-mEGFP-iPAK4-CMV-rtTA3质粒序列载体序列
LOCUS pTre3G-mEGFP-iPAK4-CMV-rtTA3 6125 bp DNA circular SYN 23-APR-2024
DEFINITION Vector for doxycycline-inducible expression of two genes in cells
containing the Tet-On(R) 3G transactivator protein.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6125)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Jul 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6125
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 1222..2298
/mol_type="genomic DNA"
/organism="."
promoter 1..451
/label=TRE3G BI promoter
/note="3rd-generation Tet-responsive bidirectional promoter
that can be activated by binding of Tet-On 3G"
primer_bind complement(58..75)
/label=Ptre3G-R
protein_bind 81..99
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 117..135
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 153..171
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 189..207
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 225..243
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 261..279
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 297..315
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 430..447
/label=Ptre3G-F
primer_bind 477..519
/label=AgeI-EGFP-F
regulatory 499..508
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 505..1221
/codon_start=1
/label=mEGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(550..571)
/label=PEGFP-N-3(EGFP-N)
primer_bind complement(550..571)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(811..830)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind complement(1107..1141)
/label=mEGFP-Qch-R
primer_bind 1107..1141
/label=mEGFP-Qch-F
misc_feature 1123..1125
/label=A to K for mEGFP
primer_bind 1158..1179
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 1200..1247
/label=GFP-iPAK4-F
primer_bind 1200..1247
/label=Primer 1a
CDS 1234..1347
/codon_start=1
/label=INKA-box
/translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
CDS 1354..2295
/codon_start=1
/label=Pak4cat
/translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
primer_bind complement(1447..1465)
/label=PAK4-R
primer_bind complement(2278..2322)
/label=NotI-Pak4-R
polyA_signal 2431..2512
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 2431..2449
/label=sv40-polyA(YK)
primer_bind complement(2572..2595)
/label=PTRE-R（A3254）(YK)
rep_origin complement(2694..3282)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3453..4313)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4227..4247
/label=Amp-R-Seq
promoter complement(4314..4418)
/gene="bla"
/label=AmpR promoter
polyA_signal 4451..4532
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4514..4532)
/label=sv40-polyA(YK)
primer_bind 4646..4693
/label=TetOn3G-NheI-R
CDS complement(4670..5416)
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
promoter complement(5519..5722)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(5548..5568)
/label=CMV-F
enhancer 5723..6102
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(6084..6122)
/label=CMV-KpnI-F
ORIGIN
1 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac
61 ctcgacatac tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
121 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
181 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
241 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
301 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
361 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
421 aactttccgt accacttcct accctcgtaa agtcgacacc ggggcccaga tctccgcggg
481 gatccatcga taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
541 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
601 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
661 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
721 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
781 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
841 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
901 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
961 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
1021 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
1081 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
1141 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
1201 ggcatggacg agctgtacaa gtcaggtgga tccgaagcag aggactggac agcagcccta
1261 ctgaacaggg gtcgcagtcg ccagcccctg gtactagggg acaattgctt tgctgacttg
1321 gtgcacaact ggatggagct gcctgaggaa ttccccgccg cccctgctgt tcctgggccc
1381 cctggccccc gctcaccaca gcgggagcca cagcgagtat cccatgagca gttccgggct
1441 gccctgcagc tggtggtgga cccaggcgac ccccgctcct acctggacaa cttcatcaag
1501 attggcgagg gctccacggg catcgtgtgc atcgccaccg tgcgcagctc gggcaagctg
1561 gtggccgtca agaagatgga cctgcgcaag cagcagaggc gcgagctgct cttcaacgag
1621 gtggtaatca tgagggacta ccagcacgag aatgtggtgg agatgtacaa cagctacctg
1681 gtgggggacg agctctgggt ggtcatggag ttcctggaag gaggcgccct caccgacatc
1741 gtcacccaca ccaggatgaa cgaggagcag atcgcagccg tgtgccttgc agtgctgcag
1801 gccctgtcgg tgctccacgc ccagggcgtc atccaccggg acatcaagag cgactcgatc
1861 ctgctgaccc atgatggcag ggtgaagctg tcagactttg ggttctgcgc ccaggtgagc
1921 aaggaagtgc cccgaaggaa gtcgctggtc ggcacgccct actggatggc cccagagctc
1981 atctcccgcc ttccctacgg gccagaggta gacatctggt cgctggggat aatggtgatt
2041 gagatggtgg acggagagcc cccctacttc aacgagccac ccctcaaagc catgaagatg
2101 attcgggaca acctgccacc ccgactgaag aacctgcaca aggtgtcgcc atccctgaag
2161 ggcttcctgg accgcctgct ggtgcgagac cctgcccagc gggccacggc agccgagctg
2221 ctgaagcacc cattcctggc caaggcaggg ccgcctgcca gcatcgtgcc cctcatgcgc
2281 cagaaccgca ccagatgacg cggccgccgg cgatatctcc agaggatcat aatcagccat
2341 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
2401 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
2461 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gccccgagct
2521 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
2581 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
2641 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
2701 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
2761 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
2821 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
2881 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
2941 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
3001 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
3061 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
3121 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
3181 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
3241 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
3301 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
3361 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
3421 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
3481 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
3541 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
3601 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
3661 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
3721 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
3781 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
3841 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
3901 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
3961 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
4021 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
4081 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
4141 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
4201 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
4261 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
4321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
4381 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
4441 cctgacgtcg gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
4501 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
4561 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
4621 gtatggctga ttatgatcct ctagacatat gctgcagacg cgtgctagct tacccgggga
4681 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
4741 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
4801 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
4861 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
4921 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
4981 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
5041 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
5101 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
5161 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
5221 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
5281 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
5341 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
5401 tgtccagtct agacatggtg aattcggggc cgcggaggct ggatcggtcc cggtgtcttc
5461 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
5521 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
5581 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
5641 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
5701 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
5761 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
5821 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
5881 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
5941 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
6001 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
6061 ccgtaattga ttactattaa taactagtca ataatcaatg tcggtacccc cggggaattc
6121 tccag
//
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