| 质粒名称: | pLZ284 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000608 |
| 产品用途: | Mammalian STAT3-specific SEAP expression vector ((OSTAT3)8-PhCMVmin-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | OSTAT3 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ284
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ284载体质粒基本信息
pLZ284质粒图谱载体图谱和pLZ284载体序列质粒序列多克隆位点信息
pLZ284质粒载体简介
pLZ284质粒序列载体序列
LOCUS pLZ284 5244 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5244)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5244
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 2561..2695
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3039..3667)
/direction=LEFT
/label=ColE1 origin
CDS complement(4112..4490)
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
promoter complement(4675..4779)
/gene="bla"
/label=AmpR promoter
primer_bind 4785..4811
/label=oYW16
misc_feature 4811..4860
/label=2XSTAT3
misc_feature 4873..4922
/label=2XSTAT3
misc_feature 4929..4978
/label=2XSTAT3
misc_feature 4985..5034
/label=2XSTAT3
primer_bind complement(5041..5068)
/label=oYW17
misc_feature 5049..5197
/label=P-hCMVmin
promoter 5071..5109
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(5182..5197)
/label=oYW11
misc_feature join(5240..5244,1..1516)
/label=SEAP
ORIGIN
1 gctgctgctg ctgctgctgg gcctgaggct acagctctcc ctgggcatca tcccagttga
61 ggaggagaac ccggacttct ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa
121 gctgcagcct gcacagacag ccgccaagaa cctcatcatc ttcctgggcg atgggatggg
181 ggtgtctacg gtgacagctg ccaggatcct aaaagggcag aagaaggaca aactggggcc
241 tgagataccc ctggccatgg accgcttccc atatgtggct ctgtccaaga catacaatgt
301 agacaaacat gtgccagaca gtggagccac agccacggcc tacctgtgcg gggtcaaggg
361 caacttccag accattggct tgagtgcagc cgcccgcttt aaccagtgca acacgacacg
421 cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt
481 ggtaaccacc acacgagtgc agcacgcctc gccagccggc acctacgccc acacggtgaa
541 ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga
601 catcgctacg cagctcatct ccaacatgga cattgacgtg atcctaggtg gaggccgaaa
661 gtacatgttt cgcatgggaa ccccagaccc tgagtaccca gatgactaca gccaaggtgg
721 gaccaggctg gacgggaaga atctggtgca ggaatggctg gcgaagcgcc agggtgcccg
781 gtatgtgtgg aaccgcactg agctcatgca ggcttccctg gacccgtctg tgacccatct
841 catgggtctc tttgagcctg gagacatgaa atacgagatc caccgagact ccacactgga
901 cccctccctg atggagatga cagaggctgc cctgcgcctg ctgagcagga acccccgcgg
961 cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt catcatgaaa gcagggctta
1021 ccgggcactg actgagacga tcatgttcga cgacgccatt gagagggcgg gccagctcac
1081 cagcgaggag gacacgctga gcctcgtcac tgccgaccac tcccacgtct tctccttcgg
1141 aggctacccc ctgcgaggga gctccatctt cgggctggcc cctggcaagg cccgggacag
1201 gaaggcctac acggtcctcc tatacggaaa cggtccaggc tatgtgctca aggacggcgc
1261 ccggccggat gttaccgaga gcgagagcgg gagccccgag tatcggcagc agtcagcagt
1321 gcccctggac gaagagaccc acgcaggcga ggacgtggcg gtgttcgcgc gcggcccgca
1381 ggcgcacctg gttcacggcg tgcaggagca gaccttcata gcgcacgtca tggccttcgc
1441 cgcctgcctg gagccctaca ccgcctgcga cctggcgccc cccgccggca ccaccgacgc
1501 cgcgcacccg ggttaacccg tggtccccgc gttgcttcct ctgctggccg ggaccctgct
1561 gctgctggag acggccactg ctccctgagt gtcccgtccc tggggctcct gcttccccat
1621 cccggagttc tcctgctccc cacctcctgt cgtcctgcct ggcctccagc ccgagtcgtc
1681 atccccggag tccctataca gaggtcctgc catggaacct tcccctcccc gtgcgctctg
1741 gggactgagc ccatgacacc aaacctgccc cttggctgct ctcggactcc ctaccccaac
1801 cccagggact gcaggttgtg ccctgtggct gcctgcaccc caggaaagga gggggctcag
1861 gccatccagc caccacctac agcccagtgg cctcgagacc tgcagcccaa gcttaaaaca
1921 gctctggggt tgtacccacc ccagaggccc acgtggcggc tagtactccg gtattgcggt
1981 acccttgtac gcctgtttta tactcccttc ccgtaactta gacgcacaaa accaagttca
2041 atagaagggg gtacaaacca gtaccaccac gaacaagcac ttctgtttcc ccggtgatgt
2101 cgtatagact gcttgcgtgg ttgaaagcga cggatccgtt atccgcttat gtacttcgag
2161 aagcccagta ccacctcgga atcttcgatg cgttgcgctc agcactcaac cccagagtgt
2221 agcttaggct gatgagtctg gacatccctc accggtgacg gtggtccagg ctgcgttggc
2281 ggcctaccta tggctaacgc catgggacgc tagttgtgaa caaggtgtga agagcctatt
2341 gagctacata agaatcctcc ggcccctgaa tgcggctaat cccaacctcg gagcaggtgg
2401 tcacaaacca gtgattggcc tgtcgtaacg cgcaagtccg tggcggaacc gactactttg
2461 ggtgtccgtg tttcctttta ttttattgtg gctgcttatg gtgacaatca cagattgtta
2521 tcataaagcg aattggattg cggccgctta agctagcttc gatccagaca tgataagata
2581 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
2641 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
2701 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
2761 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctgcctcgc gcgtttcggt
2821 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
2881 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
2941 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
3001 catcagagca gattgtactg agagtgcacc atatgtcggg ccgcgttgct ggcgtttttc
3061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
3121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
3181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
3241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
3301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
3361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
3421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
3481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
3541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
3601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
3661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
3721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
3781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
3841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
3901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
3961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
4021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
4081 agagtaagta gttcgccagt taatagtgcg caacgttgtt gccattgcta caggcatcgt
4141 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
4201 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
4261 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
4321 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
4381 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
4441 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
4501 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
4561 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
4621 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
4681 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
4741 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
4801 acctgacgtc gtcgacattt cccgtaaatc gtcgagtcga catttcccgt aaatcgtcga
4861 gggcccatcg atgtcgacat ttcccgtaaa tcgtcgagtc gacatttccc gtaaatcgtc
4921 gagggcccgt cgacatttcc cgtaaatcgt cgagtcgaca tttcccgtaa atcgtcgaat
4981 cgatgtcgac atttcccgta aatcgtcgag tcgacatttc ccgtaaatcg tcgaacgcgt
5041 cctgcaggtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga ggcctatata
5101 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
5161 ctccatagaa gacaccggga ccgatccagc ctccgcggaa ttcgagctcg cccggggatc
5221 ctctagagtc agcttctgca tgct
//
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