pAAV-AscI-CAG-H2B-Nelle-L3-mCherry

LOCUS       pAAV-AscI-CAG-H2B-Nelle-L3-mCherry              7705 bp DNA     circular SYN 15-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-AscI-CAG-H2B-NeIle-L3-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Vax
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2661..2691
                     /label=AscI-OxE insert
                     /label=AscI-OxE\insert
                     /note="/vntifkey=21"
     misc_feature    2692..2935
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="/vntifkey=21"
     misc_feature    2942..3219
                     /label=chicken b-actin promoter
                     /label=chicken\b-actin\promoter
                     /note="/vntifkey=21"
     CDS             3646..4023
                     /codon_start=1
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             4030..5814
                     /codon_start=1
                     /label=NeIle
                     /note="/vntifkey=21"
                     /translation="MEDIKVAVVGAMSAGADWRISKEACPNGKTLISTLKWSYTTGDGK
                     RYRSTARTTFTFAKPMAANYLKNQPMYVFRRTELKHSKTEMDFKEWQKAFTDVVGMDEL
                     YKVDGGSGGTGMVSEGEVDNMASLPATHELHIFGSINGVGFDMVGQGSGNPNVGYEELN
                     LKSTKGELQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTVRFEDGAS
                     LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAFHIGPVTQYGDQEFTGAEQAV
                     ADINARGGIKGNKLQIVKYDDACDPKQAVAVANRVVNDGIKYVIGHLCSSSAQPASDIY
                     EDAGILMITPAATAPELTARGYQLILRTTGLDSDQGLTAAKYILEKVKPQRIAIVHDKQ
                     QYGEGLARAVQGGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMG
                     QILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKHIVDAVK
                     AKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLK
                     GFEFGVFDWHANGTATDAK"
     CDS             5878..6588
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    6607..7195
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    7238..7359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(7705,1..940)
                     /label=ColE1
                     /note="ColE1"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
     2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     3241 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     3301 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     3361 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     3421 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
     3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     3601 gctgtctcat cattttggca aagaattgga tccaccggtg acaccatgcc agagccagcg
     3661 aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa
     3721 ggcggcaaga agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt
     3781 ctgaagcagg tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg
     3841 tttgtgaacg acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac
     3901 aagcgctcga ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg
     3961 gagttggcca agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct
     4021 aagagatcta tggaagatat taaagtcgcg gtcgtgggcg caatgtccgc tggtgctgac
     4081 tggcgtatat ccaaggaggc ttgccccaat ggtaagactc ttatttccac acttaagtgg
     4141 tcttatacca ccggcgacgg caaacgctac cggtctactg ctcgcaccac attcacattc
     4201 gccaaaccaa tggccgcaaa ttacctcaaa aatcagccga tgtatgtctt ccggagaaca
     4261 gagctgaaac attctaagac cgaaatggat tttaaagagt ggcaaaaggc ctttacggac
     4321 gtggtgggca tggacgagct gtacaaggtg gatggcggca gcggtggcac cggcatggtc
     4381 tcagagggag aggtggacaa catggctagt ctgcctgcta cccacgaact gcatattttt
     4441 ggctccataa atggcgtcgg tttcgacatg gtaggccaag gctccggtaa tccaaacgtc
     4501 ggttatgagg agctgaacct gaagtccacc aagggcgaac tgcagttcag tccctggatc
     4561 ctggtgccgc acattgggta tgggtttcat cagtatcttc cctacccgga cgggatgtcg
     4621 ccatttcagg ctgcaatggt cgacgggtcc ggatatcagg tgcaccgcac tgtgcggttt
     4681 gaggacggtg cttcactgac cgtgaattac agatacacct acgaaggatc acatatcaaa
     4741 ggggaagctc aggtgaaggg cactgggttc ccggcagacg gccctgtgat gacaaattcc
     4801 ctgacagcct ttcatattgg tccggtgacc cagtacggtg accaggagtt taccggcgca
     4861 gagcaggcgg ttgcggatat caacgctaga ggcggcatta agggcaacaa actgcaaatc
     4921 gtaaaatatg acgatgcctg tgacccgaaa caggctgttg cggtggcgaa cagagtcgtt
     4981 aacgacggca ttaaatatgt gattggtcac ctctgttctt catcagcgca gcctgcgtct
     5041 gacatctacg aagacgcagg cattttaatg atcaccccag cggcaaccgc gccggagctg
     5101 accgcccgtg gctatcagct gatcctgcgc accaccggcc tggactccga ccaggggctg
     5161 acggcggcga aatatattct tgagaaagtg aaaccgcagc gtattgctat cgttcacgac
     5221 aaacagcagt acggcgaagg tctggcgcga gcggtgcagg gtggcctgaa gaaaggcaat
     5281 gcaaacgtgg tgttctttga tggcatcacc gccggggaaa aagatttctc aacgctggtg
     5341 gcgcgtctga aaaaagagaa tatcgacttc gtttactacg gcggttatca cccggaaatg
     5401 gggcaaatcc tgcgtcaggc acgcgcggca gggctgaaaa ctcagtttat ggggccggag
     5461 ggtgtggcta acgtttcgct gtctaacatt gcgggcgaat cagcggaagg gctgctggtg
     5521 accaagccga agaactacga tcaggttccg gcgaacaaac acattgttga cgcagtcaaa
     5581 gcgaaaaaac aggacccaag tggcgcattc gtttggacca cctacgccgc gctgcaatct
     5641 ttgcaggcgg gcctgaatca gtctgacgat ccggctgaaa tcgccaaata cctgaaagcg
     5701 aactccgtgg ataccgtaat gggaccgctg acctgggatg agaaaggcga tctgaaaggc
     5761 tttgagttcg gcgtatttga ctggcacgcc aacggcacgg cgaccgatgc gaaggaattc
     5821 ggtggttctg gttctggagg gagtggcagc aagcttggtg gttctggttc tggtaccatg
     5881 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     5941 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     6001 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     6061 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     6121 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     6181 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     6241 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     6301 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     6361 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     6421 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     6481 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     6541 gccgagggcc gccactccac cggcggcatg gacgagctgt ataagtaaac tagtaagctt
     6601 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6661 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6721 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6781 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6841 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6901 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6961 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7021 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7081 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7141 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     7201 taccgtcgac ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     7261 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     7321 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     7381 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     7441 aaatgtggta aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg
     7501 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     7561 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     7621 ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg
     7681 agaggcggtt tgcgtattgg gcgct
//