PCDH-CMV-mCherry-TRIM67

LOCUS      PCDH-CMV-mCherry-TRIM67             9501 bp DNA     circular SYN 03-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3220..3245
                     /label=TRIM19-F
     primer_bind     3220..3244
                     /label=TRIM18-F
     primer_bind     3221..3259
                     /label=TRIM67-f
     primer_bind     3221..3243
                     /label=trim66-F
     misc_feature    3240..5588
                     /label=trim67
     primer_bind     complement(4287..4314)
                     /label=TRIM67 L355 V358-R
     primer_bind     4297..4331
                     /label=TRIM67 L355 V358-F
     misc_feature    4303
                     /label=Feature 24
     misc_feature    4311..4312
                     /label=Feature 25
     primer_bind     complement(4641..4653)
                     /label=TRIM67 L355 V358-R
     CDS             complement(5342..5353)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5583..5610)
                     /label=TRIM67-R
     primer_bind     complement(5592..5611)
                     /label=TRIM18-R
     primer_bind     complement(5592..5611)
                     /label=TRIM19-R
     primer_bind     complement(5592..5611)
                     /label=TRIM66-R
     misc_feature    5598..6185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6069..6080)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6258..6491
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6563..6684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6703..6838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6876..6892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6900..6916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6924..6954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6969..6990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7278..7866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8037..8897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8898..9002)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9371..9384)
                     /label=Primer 5
     primer_bind     9476..9492
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggaggagga gctgaagtgc cccgtgtgcg gcagcctgtt cagagagccc atcatcctgc
     3301 cctgcagcca caatgtgtgc ctgccctgtg ccagaaccat cgccgtgcag acacctgatg
     3361 gcgagcagca cctgccccag cccctgctgc tgtccagagg ctccggcctg caggccggag
     3421 ctgctgctgc tgcatccctg gagcacgacg ccgccgctgg acctgcttgt ggcggagctg
     3481 gcggcagcgc tgctggagga ctgggaggag gcgccggagg aggaggagat cacgccgata
     3541 agctgagcct gtacagcgag acagattccg gctacggcag ctacacaccc tccctgaagt
     3601 cccccaatgg cgtgagggtg ctgcctatgg ttcccgcccc ccctggctcc agcgctgctg
     3661 ctgccagagg cgctgcctgc tccagcctga gcagctccag ctcctccatc acctgccccc
     3721 agtgccacag gtccgccagc ctggaccaca ggggcctgag aggctttcag agaaacaggc
     3781 tgctggaggc catcgtgcag agataccagc agggcagggg cgccgtgccc ggaaccagcg
     3841 ctgctgccgc tgtggctatc tgtcagctgt gtgacaggac ccctcccgag cccgccgcta
     3901 cactgtgcga gcagtgtgat gtgctgtact gtagcgcctg ccagctgaag tgccacccca
     3961 gcagaggccc ctttgccaag cacagactgg tgcagccccc ccctcctcct ccccctcctg
     4021 ctgaagccgc cagcggacca acaggcacag cccagggcgc cccatccggt ggaggaggat
     4081 gtaagagccc cggcggcgcc ggagctggag ctaccggagg aagcacagcc agaaagttcc
     4141 ctacatgccc cgagcacgag atggagaact acagcatgta ctgtgtgtcc tgcaggacac
     4201 ctgtgtgcta cctgtgcctg gaggagggca gacacgccaa gcacgaggtg aagcccctgg
     4261 gcgccatgtg gaagcagcac aaggcccagc tgagccaggc cccgaacggc ccgagcgaca
     4321 aggccaagga ggccaaggag ttcctggtgc agctgaagaa tatcctgcag cagatccagg
     4381 agaacggcct ggattacgag gcctgtctgg tggcccagtg tgacgccctg gtggatgccc
     4441 tgaccaggca gaaggccaag ctgctgacca aggtgaccaa ggagagggag cacaagctga
     4501 agatggtgtg ggatcagatc aaccactgta ccctgaagct gagacagtcc accggcctga
     4561 tggagtactg cctggaggtc attaaggaga acgacccttc cggcttcctg cagatctccg
     4621 acgccctgat caagagggtg caggtgagcc aggagcagtg ggtgaagggc gccctggagc
     4681 ctaaggtgtc cgccgagttt gatctgaccc tggactccga gcccctgctg caggccatcc
     4741 accagctgga cttcatccag atgaagtgta gggtgccccc tgtgcccctg ctgcaactgg
     4801 agaagtgctg cacaagaaac aactccgtga cactggcctg gagaatgccc cccttcacac
     4861 actcccccgt ggacggctac atcctggagc tggatgatgg cgccggcggc cagtttagag
     4921 aggtgtacgt gggcaaggag acactgtgca ccatcgacgg cctgcacttt aactccacct
     4981 acaatgccag ggtgaaggcc ttcaatagca gcggcgtggg cccttactcc aagacagtgg
     5041 tgctgcagac ctccgacgtg gcctggttca ccttcgaccc taacagcggc cacagggaca
     5101 tcatcctgag caacgacaat cagaccgcca cctgttccag ctacgacgac agagtggtgc
     5161 tgggcacagc cgccttcagc aagggcgtgc actactggga gctgcacgtg gatagatacg
     5221 ataatcaccc cgatcccgcc tttggcgtgg ccagggcttc cgtggtgaag gatatgatgc
     5281 tgggcaagga tgataaggcc tgggccatgt acgtggacaa taacaggtcc tggtttatgc
     5341 actgcaactc ccacaccaac agaacagagg gcggcgtgtg taagggcgcc acagtgggcg
     5401 tgctgctgga tctgaataag cacacactga catttttcat caacggccag cagcagggcc
     5461 ccacagcctt cagccacgtg gacggcgtgt ttatgcccgc cctgtccctg aatagaaacg
     5521 tgcaggtgac cctgcacacc ggcctggagg tgcccaccaa cctgggcagg cctaagctgt
     5581 ccggcaatta gggatccaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5641 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5701 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5761 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5821 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5881 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5941 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     6001 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6061 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6121 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc
     6181 cccgccggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6241 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     6301 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6481 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6541 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6601 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6661 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6721 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6781 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6841 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6901 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6961 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7021 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7081 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7681 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8041 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8101 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8161 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8221 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8281 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8341 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8401 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8461 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8521 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8581 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8641 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8701 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8761 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8821 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8881 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8941 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9001 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     9061 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     9121 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     9181 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     9241 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9301 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     9361 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     9421 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     9481 acgacggcca gtgccaagct g
//