pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX

LOCUS       pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX             8684 bp DNA     circular SYN 23-JAN-2024
DEFINITION  Expresses the genetically-encoded fluorescent dopamine (DA) sensor 
            GRAB_gDA3h and a membrane-localized mcherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8684)
  TITLE     Improved dual-color GRAB sensors for monitoring dopaminergic 
            activity in vivo
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     642..661
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        642..660
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             741..2879
                     /codon_start=1
                     /label=gDA3h
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGRTLNTSTMEGTGL
                     VAERDFSFRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLV
                     AVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPGRYE
                     RKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTGPSEGNATSLGKTINNCDSSLS
                     RTYAISSSLISFYIPVAIMIVTYTRIYRIAQKQIRRISAGPRDTDALDLEEGGNVYIKA
                     DKQKNGIKANFTIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNE
                     KRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKF
                     SVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKS
                     AMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNT
                     GAAARWSSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCMVPFCGSGETKPFCIDS
                     ITFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPTTNNAIETVSINNNGAV
                     VFSSHHEPRGSISKDCNVVYLIPHAVGSSEGLKKEEAVGIAKPLEKLSPALSVILDYDT
                     DVSLEKIQPITQNGQHPT"
     sig_peptide     741..803
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    804..828
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1772..1793
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1899..1920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2160..2179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2887..3472
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3054..3071)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3280..3299
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3473..4180
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3618..3636)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3886..3905
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4238..4267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4271..4417
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4439..4456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4445..4669
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4801..5389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4881..4900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5718..5765
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5790..5809
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5997..6791)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6108..6127)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6718..6737
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6890..6909)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6988..7179
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7136..7156
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(7239..8099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7862..7881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8100..8204)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(8231..8684,1..2)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8318..8337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8528..8549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttacgcgcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ctgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      601 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      661 gagacccaag cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat
      721 tcggcttggg gatatccacc atggagacag acacactcct gctatgggta ctgctgctct
      781 gggttccagg ttccactggt gacacaagtt tgtacaaaaa agttggcacc accggtagga
      841 ctctcaacac gtctaccatg gaaggcaccg ggctggtggc ggagagggac ttctccttcc
      901 gcattctcac agcctgtttc ctgtcgctgc ttatcctgtc caccctcctg gggaacacgc
      961 tggtctgtgc cgccgtgatc aggttccgcc acctgcgatc caaggtgacc aacttcttcg
     1021 tcatctcctt ggctgtatcg gatctcttgg tggccgtctt ggtcatgccc tggaaagcag
     1081 tggccgagat cgctggcttc tggcccttcg ggtccttctg taacatctgg gtggcctttg
     1141 acatcatgtg ctccaccgca tccatcctca atctctgtgt gatcagtgtg gacaggtatt
     1201 gggccatctc tagccccggt cggtatgaga ggaagatgac ccccaaggca gccttcattc
     1261 tgatcagtgt ggcatggact ttgtcggttc ttatctcctt catccccgtg cagctcagct
     1321 ggcacaaggc gaaacccaca ggtccctctg aggggaatgc cacttccctg ggcaagacca
     1381 tcaacaactg tgactccagc ttgagcagga catatgccat ttcatcctct ctaataagct
     1441 tttacatccc tgtggccatc atgattgtca cctacaccag gatctacagg atcgcccaga
     1501 aacaaatacg gcgtatctca gccgggccgc gcgacaccga cgcgctggac ctggaggagg
     1561 gaggaaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttcacga
     1621 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc
     1681 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac
     1741 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1801 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga
     1861 gaaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1921 taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc gagggcaagc
     1981 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2041 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2101 acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg
     2161 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2221 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2281 agtacaacac cggagcagca gcacgctgga gctcctttaa gatgtccttc aaaagagaga
     2341 ctaaagttct gaagactctg tcggtgatca tgggggtgtt cgtgtgctgc tggctccctt
     2401 tcttcatctt gaactgcatg gtgcccttct gtgggtctgg agagaccaag cccttctgca
     2461 tcgattccat cacctttgac gtgtttgtgt ggtttgggtg ggctaattcc tccttgaacc
     2521 ccatcattta tgcctttaat gctgattttc ggaaggcatt ttccaccctc ttaggatgct
     2581 acaggctttg cccgacgacg aataatgcca tagagacggt tagcatcaat aacaatgggg
     2641 ctgtggtgtt ttccagccat cacgagcctc ggggttccat ctccaaggac tgcaatgtgg
     2701 tgtatctgat cccacacgct gtgggctcct ctgagggcct gaagaaggag gaggcggtgg
     2761 gaatagccaa gcccttggag aagctgtccc cagccctgtc tgtcattttg gactatgaca
     2821 ctgacgtctc tcttgagaag atccagccca tcacacaaaa cgggcagcac ccgacctgag
     2881 ctagcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     2941 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     3001 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     3061 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     3121 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     3181 gtgcctctgc ggccaacgcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     3241 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     3301 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     3361 cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     3421 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag
     3481 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     3541 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3601 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     3661 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     3721 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3781 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     3841 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3901 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     3961 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4021 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4081 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     4141 gggccgccac tccaccggcg gcatggacga gctgtacaag gatggtaaaa agaagaaaaa
     4201 gaagtcaaag acaaagtgtg taattatgta agtcgacgaa caaaaactca tctcagaaga
     4261 ggatctgaat gctgtgggcc aggacacgca ggaggtcatc gtggtgccac actccttgcc
     4321 ctttaaggtg gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct
     4381 tatcatcctc atcatgcttt ggcagaagaa gccacgttag gcggccgctc gagatcagcc
     4441 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4501 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4561 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4621 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4681 gaaagaacca gtggcggtaa tacggttatc cacagaatcg ggggataacg caggaaagaa
     4741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5101 ctatcgtctt gagtccaacc cggtaagacg cgacttatcg ccactggcag cagccactgg
     5161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5221 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5521 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     5581 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     5641 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     5701 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     5761 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     5821 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5881 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5941 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     6001 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6061 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     6121 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     6181 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccata ggtcacgacg
     6241 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     6301 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6361 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6421 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6481 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6541 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6601 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6661 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6721 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6781 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     6841 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgggc ctcggcctct
     6901 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     6961 aggggcggaa tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     7021 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     7081 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     7141 aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt tttcaataaa
     7201 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7261 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7321 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7381 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7441 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7501 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7561 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7621 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7681 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7741 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7801 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7861 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7921 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7981 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8041 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8101 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8161 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8221 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8281 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8341 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8401 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8461 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8521 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8581 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8641 caaaaattta acgcgaattt taacaaaata ttaacgctta caat
//