pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

LOCUS       pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro                9453 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Gordon DE, et al.
  TITLE     A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug 
            Targets and Potential Drug Repurposing
  JOURNAL   BioRxiv March 2020
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1182
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1207..1216
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1771..1794
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1831..1854
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    1866..2439
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2033..2050)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2260..2279
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2472..3071
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2472..2491)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2968..2988
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3085..3673
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3138..3158)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3556..3567)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3880..4513
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4641..4657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4641..4657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4654..4676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4665..4681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4689..4719)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4734..4755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4872..4889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5043..5631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5123..5142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5802..6662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6425..6444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6663..6767)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6815..6949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6852..6871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6906..6925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7117..7750
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7797..7922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8419..8652
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8837..8881
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9144..9259
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
     1201 ttcgccgcca ccatggagag tcttgtaccc ggctttaacg agaaaacaca tgttcaattg
     1261 tcattgcctg tgctgcaagt ccgggacgta ctggttaggg ggttcggaga ttcagtagaa
     1321 gaggtgctgt ctgaggcgag gcaacatttg aaagacggga cgtgcgggct ggttgaggtg
     1381 gaaaagggag tacttccaca gttggaacaa ccgtatgttt ttattaagcg gagtgatgcc
     1441 aggactgcgc cgcacggtca tgtcatggtt gagcttgtcg cagagcttga aggcattcaa
     1501 tatgggcgct ccggtgaaac tcttggagta cttgtgccgc acgtaggcga aattccagtg
     1561 gcatatcgga aggtgctgtt gagaaaaaat gggaacaagg gcgcaggggg ccacagttat
     1621 ggagccgacc tcaaatcctt tgaccttggc gacgagttgg ggacagatcc ctacgaggat
     1681 ttccaggaaa actggaatac gaaacatagc agtggcgtta ctcgcgagtt gatgcgcgaa
     1741 ctgaacggcg gcctcgaagg cggcggggga tggagccatc cacaattcga gaaaggcggt
     1801 ggttcaggag gaggtagcgg gggtggatca tggtcacatc cgcagtttga aaagtaagga
     1861 tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     1921 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     1981 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     2041 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     2101 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     2161 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     2221 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     2281 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     2341 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     2401 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga
     2461 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     2521 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     2581 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     2641 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     2701 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     2761 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     2821 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     2881 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     2941 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     3001 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     3061 ccggtgccta gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     3121 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3181 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3241 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3301 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3361 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3421 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3481 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3541 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3601 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3661 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     3721 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     3781 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     3841 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     3901 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     3961 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     4021 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     4081 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     4141 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     4201 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     4261 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     4321 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4501 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     4561 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     4621 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4681 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4741 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4801 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4861 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6781 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
     6841 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6901 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     6961 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     7021 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     7081 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     7141 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     7201 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     7261 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     7321 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     7381 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     7441 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     7501 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     7561 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7741 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7801 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7861 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7921 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7981 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8041 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8101 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8161 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8221 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     8281 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8341 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8401 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8461 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8521 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8581 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8641 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8701 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8761 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8821 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8881 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8941 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9001 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9061 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9121 gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag
     9181 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9241 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat
     9301 gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa
     9361 ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc
     9421 gtcatcactg aggtggagaa gagcatgcgt gag
//