pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

LOCUS       pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro               10280 bp ds-DNA     circular SYN 26-JAN-2024
DEFINITION  Expression of one half of a PKAsub-mCherry-MinD reporter circuit in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
  TITLE     A programmable reaction-diffusion system for spatiotemporal cell 
            signaling circuit design.
  JOURNAL   Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 
            10.1016/j.cell.2023.12.007.
  PUBMED    38181787
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2024 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     346..366
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    473..1061
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(526..546)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(944..955)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1268..1901
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2029..2045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2029..2045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2042..2064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2053..2069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2077..2107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2122..2143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2260..2277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2431..3019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2511..2530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3190..4050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3813..3832
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4051..4155)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4203..4337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4240..4259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4294..4313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4505..5138
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5185..5310
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5807..6040
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6225..6269
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6532..6647
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        6725..6936
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          6937..7875
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7832..7852
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7924..8631
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8069..8087)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8337..8356
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(9722..9739)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9949..9968
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10130..449
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10130..10149)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
       61 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
      121 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
      181 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
      241 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
      301 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
      361 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
      421 ggtgcatgac ccgcaagccc ggtgcctgac ccgggcgggg cgcgtctgga acaatcaacc
      481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
      541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
      601 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
      661 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
      721 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
      781 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
      841 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
      901 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
      961 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     1021 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     1081 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     1141 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     1201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     1261 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     1321 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     1381 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     1441 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     1501 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     1561 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     1621 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     1681 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     1741 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1801 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     1861 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     1921 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     1981 ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     2041 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2101 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2161 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3061 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     4201 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4321 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     4381 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     4441 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     4501 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     4561 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     4621 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     4681 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     4741 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     4801 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     4861 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     4921 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     4981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     5161 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     5221 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     5281 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     5341 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     5401 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     5461 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     5521 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     5581 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     5641 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     5701 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     5761 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     5821 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     5881 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     5941 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     6001 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6061 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6121 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     6181 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     6241 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     6301 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     6361 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     6421 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     6481 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga
     6541 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     6601 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     6661 agggacagca gagatccagt ttatcgatga ggccctttcg tcttcactcg aggtgcccgt
     6721 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6781 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6841 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6901 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     6961 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     7021 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     7081 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     7141 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     7201 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     7261 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     7321 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     7381 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     7441 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     7501 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     7561 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     7621 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     7681 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     7741 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     7801 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     7861 tttcttccat ttcaggtgtc gtgaggatcc gccaccatgt tgaggcgcgc gacgctggtt
     7921 gacatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     7981 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     8041 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     8101 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     8161 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     8221 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     8281 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     8341 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     8401 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     8461 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     8521 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     8581 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa ggacagcgcc
     8641 ggaagtgctg gctctgcggg taccatggcc cgcataatag ttgtgacttc aggtaagggc
     8701 ggagttggaa aaaccacatc cagcgcagcc atagctaccg gcctcgcaca gaaaggcaag
     8761 aaaactgtgg tcatagattt tgatattggg ctcaggaacc tggacctgat aatgggctgc
     8821 gaacggcgcg ttgtctacga ttttgtcaat gtcatccaag gcgacgcgac tcttaatcag
     8881 gcacttataa aagataaacg aaccgaaaac ttgtatattc ttcctgcctc acaaacgcgg
     8941 gacaaagacg cgctcaccag ggagggtgtc gcaaaagttt tggatgactt gaaagcgatg
     9001 gactttgagt tcatagtttg tgactctccc gccggtatag agacaggggc acttatggca
     9061 ctgtacttcg cagatgaagc cataatcaca actaatccgg aggtgtctag tgtgcgagat
     9121 tcagaccgaa tactggggat actcgccagc aaatcacgga gggcagaaaa cggggaggag
     9181 ccaattaagg agcatctcct gcttacccga tataaccccg gtcgagtatc tcggggagat
     9241 atgctttcaa tggaagatgt cctcgagatt ctgaggataa aactggtagg cgttatcccg
     9301 gaagaccagt ccgtcctccg cgcatctaat cagggggagc ctgtcatact tgacataaat
     9361 gcggatgctg ggaaagctta tgccgatacg gtagaacggc tcctcgggga agaacggccc
     9421 ttccgcttca tagaggagga aaagaaggga ttccttaaac ggctgttcgg gggataagcg
     9481 gccgctctag agtcgacggg ccgcggtaac aattgttaac taacttaagc tagcaacggt
     9541 ttccctctag cgggatcaat tccgcccccc ccccctaacg ttactggccg aagccgcttg
     9601 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     9661 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     9721 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     9781 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     9841 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     9901 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     9961 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
    10021 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
    10081 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tgaccgagta
    10141 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
    10201 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
    10261 gcgggtcacc gagctgcaag
//