PCDH-CMV-mCherry-TRIM66

LOCUS      PCDH-CMV-mCherry-TRIM66      10800 bp DNA     circular SYN 29-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2878..2888)
                     /label=TRIM65-F
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3218..3245
                     /label=TRIM65-F
     misc_feature    3240..6887
                     /label=trim66 o
     primer_bind     complement(6888..6908)
                     /label=TRIM65-R
     misc_feature    6897..7484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7368..7379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7557..7790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7862..7983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8002..8137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8175..8191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8199..8215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8223..8253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8268..8289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8577..9165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9336..10196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10197..10301)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10670..10683)
                     /label=Primer 5
     primer_bind     10775..10791
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggccaggaa ctgcagcgag tgcaaggaga agagggccgc ccacatcctg tgcacctact
     3301 gcaataggtg gctgtgcagc tcctgtacag aggagcacag acacagccct gtgcccggcg
     3361 gcccattctt tcctagagcc cagaagggct cccccggcgt gaacggaggc ccaggagatt
     3421 tcaccctgta ctgccccctg cacacacagg aggtgctgaa gctgttctgt gagacatgcg
     3481 acatgctgac atgccacagc tgtctggtgg tggagcacaa ggagcacaga tgcaggcacg
     3541 tggaggaggt gctgcagaac cagaggatgc tgctggaggg cgtgaccacc caggtggccc
     3601 acaagaagtc cagcctgcag acaagcgcca agcagatcga ggataggatc ttcgaggtga
     3661 agcaccagca caggaaggtg gagaatcaga tcaagatggc caagatggtg ctgatgaacg
     3721 agctgaacaa gcaggccaac ggcctgatcg aggagctgga gggcatcaca aatgagagaa
     3781 agaggaagct ggagcagcag ctgcagagca tcatggtgct gaatagacag tttgagcacg
     3841 tgcagaattt tatcaactgg gccgtgtgct ccaagacaag cgtgcccttc ctgttctcca
     3901 aggagctgat cgtgttccag atgcagagac tgctggagac atcctgcaat acagatcccg
     3961 gctccccttg gagcatcagg ttcacctggg agcctaattt ttggaccaag cagctggcct
     4021 ccctgggctg catcaccacc gagggcggcc agatgtccag agccgacgcc cctgcctacg
     4081 gcggactgca aggctcctcc cccttctacc agtcccacca gtcccccgtg gcccagcagg
     4141 aggctctgag ccacccttcc cacaagtttc agagccccgc cgtgtgcagc agcagcgtgt
     4201 gttgcagcca ctgttccccc gtgagcccta gcctgaaggg ccaggtgccc ccccctagca
     4261 tccaccctgc ccactccttc agacagcctc ctgagatggt gccccagcag ctgggcagcc
     4321 tgcagtgtag cgccctgctg cccagagaga aggagctggc ctgttccccc caccccccta
     4381 agctgctgca gccttggctg gagacacagc ctcctgtgga gcaggagtcc acaagccaga
     4441 ggctgggcca gcagctgacc agccagcccg tgtgcatcgt gcctcctcag gatgtgcagc
     4501 agggcgccca cgcccagcca acactgcaga cccccagcat ccaggtgcag ttcggccacc
     4561 accagaagct gaagctgtcc cacttccagc agcagcctca gcagcagctg ccccctcctc
     4621 cccctccact gccacaccca ccccctcctc tgccccctcc acctcagcag ccccacccac
     4681 ctctgcctcc tagccagcac ctggccagct cccagcacga gagcccccct ggacccgctt
     4741 gctcccagaa catggatatc atgcaccaca agtttgagct ggaggagatg cagaaggatc
     4801 tggagctgct gctgcaggcc cagcagccta gcctgcagct gagccagacc aagagccctc
     4861 agcacctgca gcagaccatc gtgggccaga tcaattacat cgtgagacag cctgcccctg
     4921 tgcagagcca gtcccaggag gagacactgc aggccaccga cgagccccct gcctctcagg
     4981 gaagcaagcc cgccctgcct ctggataaga ataccgccgc cgccctgcct caggcctccg
     5041 gtgaggaaac ccctctgtcc gtgccccctg tggatagcac catccagcac agctccccta
     5101 atgtggtgag gaagcacagc acatccctgt ccatcatggg cttcagcaac accctggaga
     5161 tggagctgtc cagcaccagg ctggagaggc ccctggagcc ccagatccag agcgtgagca
     5221 atctgacagc cggcgccccc caggccgtgc ctagcctgct gtctgcccct cccaagatgg
     5281 tgagctccct gacaagcgtg cagaatcagg ccatgccttc cctgaccaca agccacctgc
     5341 agaccgtgcc ttccctggtg cacagcacat ttcagagcat gcctaacctg atcagcgatt
     5401 ccccccaggc tatggcttcc ctggcctccg atcaccccca ggccggacct tccctgatga
     5461 gcggccacac acaggccgtg ccaagcctgg ccacatgccc cctgcagagc attcctcccg
     5521 tgtccgacat gcagcctgag acaggcagct ccagcagctc cggcaggaca agcggcagcc
     5581 tgtgccctag agatggcgcc gatcccagcc tggagaacgc cctgtgcaag gtgaagctgg
     5641 aggagcccat caacctgagc gtgaagaagc ctcccctggc ccctgtggtg tccacctcca
     5701 ccgccctgca gcagtaccag aatcccaagg agtgcgagaa tttcgagcag ggcgccctgg
     5761 agctggacgc caaggagaac cagagcatca gggcctttaa tagcgagcac aagatccctt
     5821 acgtgagact ggagaggctg aagatctgcg ccgccagcag cggcgagatg cccgttttca
     5881 agctgaagcc tcagaagaac gaccaggacg gctcctttct gctgatcatc gagtgtggca
     5941 ccgagtcctc cagcatgagc atcaaggtga gccaggatag gctgagcgag gccacacagg
     6001 cccccggact ggagggaagg aaggtgacag tgacaagcct ggccggccag aggcctcccg
     6061 aggttgaggg aacaagccct gaggagcaca ggctgatccc tagaacaccc ggcgccaaga
     6121 agggcccccc tgctcctatc gagaacgagg acttttgcgc cgtgtgcctg aacggcggcg
     6181 agctgctgtg ttgtgacaga tgtcccaagg tgttccacct gagctgtcac gtgcccgccc
     6241 tgctgtcctt tcctggcggc gagtgggtgt gcacactgtg cagaagcctg acccagcccg
     6301 agatggagta cgactgcgag aatgcctgct acaaccagcc cggcatgagg gcctcccctg
     6361 gactgagcat gtacgatcag aagaagtgtg agaagctggt gctgagcctg tgctgcaaca
     6421 acctgagcct gcctttccac gagcctgtga gccccctggc cagacactac taccagatca
     6481 tcaagaggcc tatggatctg tccatcatca gaagaaagct gcagaagaag gaccccgccc
     6541 actacaccac acccgaggag gtggtgtccg acgtgaggct gatgttctgg aactgcgcca
     6601 agtttaacta ccccgatagc gaggtggccg aggccggcag atgtctggag gtgttctttg
     6661 agggctggct gaaggagatc taccctgaga agaggtttgc ccagcccagg caggaggact
     6721 ccgattccga ggaggtgagc tccgagtccg gctgttccac cccccagggc ttcccttggc
     6781 ccccatacat gcaggagggc atccagccca agaggagaag gagacacatg gagaacgaga
     6841 gagccaagag gatgtccttc aggctggcca atagcatcag ccaggtgtag ggatccaatc
     6901 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6961 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7021 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7081 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7141 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7201 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7261 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7321 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7381 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc
     7441 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgccggtac ctttaagacc
     7501 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7561 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     7621 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7681 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7741 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7801 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7861 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7981 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     8041 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8101 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     8161 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8221 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8281 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8341 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8401 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8461 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8521 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8581 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8641 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8701 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8761 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8821 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8881 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8941 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9001 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9061 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9241 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9301 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9361 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9421 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9481 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9541 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9601 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9661 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9721 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9781 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9841 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9901 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9961 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10021 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10081 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10141 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10201 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10261 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10321 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10381 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    10441 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    10501 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
    10561 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10621 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    10681 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    10741 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//