pAAVLINKv2-SCP1-Cre-UDeg3a-ATM-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-ATM-C-FLAG                7183 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3896
                     /codon_start=1
                     /gene="ATM"
                     /gene_synonym="AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1"
                     /product="serine-protein kinase ATM isoform a"
                     /EC_number="2.7.11.1"
                     /label=ATM
                     /note="isoform a is encoded by transcript variant 2; TEL1, 
                     telomere maintenance 1, homolog; AT mutated; serine-protein
                     kinase ATM; A-T mutated; ataxia telangiectasia mutated; 
                     serine/threonine kinase ATM"
                     /db_xref="GI:71902540"
                     /db_xref="CCDS:CCDS31669.1"
                     /db_xref="GeneID:472"
                     /db_xref="HGNC:HGNC:795"
                     /db_xref="MIM:607585"
                     /protein_id="NP_000042.3"
                     /translation="AIFQIKQYNSVSCGVSEWQLEEAQVFWAKKEQSLALSILKQMIKK
                     LDASCAANNPSLKLTYTECLRVCGNWLAETCLENPAVIMQTYLEKAVEVAGNYDGESSD
                     ELRNGKMKAFLSLARFSDTQYQRIENYMKSSEFENKQALLKRAKEEVGLLREHKIQTNR
                     YTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENS
                     GVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPH
                     HTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRR
                     PQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVD
                     HTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDGKERRQLVKGRDDLRQDAVMQQV
                     FQMCNTLLQRNTETRKRKLTICTYKVVPLSQRSGVLEWCTGTVPIGEFLVNNEDGAHKR
                     YRPNDFSAFQCQKKMMEVQKKSFEEKYEVFMDVCQNFQPVFRYFCMEKFLDPAIWFEKR
                     LAYTRSVATSSIVGYILGLGDRHVQNILINEQSAELVHIDLGVAFEQGKILPTPETVPF
                     RLTRDIVDGMGITGVEGVFRRCCEKTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKA
                     LYLQQRPEDETELHPTLNADDQECKRNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVL
                     SVGGQVNLLIQQAIDPKNLSRLFPGWKAWV"
     misc_feature    2807..2812
                     /label=SDS
     CDS             3903..3968
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3972..4403
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4116..4121
                     /label=SDS
     repeat_region   4446..4586
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4446..4575
                     /label=AAV2 ITR
     repeat_region   4447..4587
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4661..5116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5398..5502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5503..6363
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6534..7122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca atatttcaaa
     1561 ttaaacagta caattcagtt agctgtggag tctctgagtg gcagctggaa gaagcacaag
     1621 tattctgggc aaaaaaggag cagagtcttg ccctgagtat tctcaagcaa atgatcaaga
     1681 agttggatgc cagctgtgca gcgaacaatc ccagcctaaa acttacatac acagaatgtc
     1741 tgagggtttg tggcaactgg ttagcagaaa cgtgcttaga aaatcctgcg gtcatcatgc
     1801 agacctatct agaaaaggca gtagaagttg ctggaaatta tgatggagaa agtagtgatg
     1861 agctaagaaa tggaaaaatg aaggcatttc tctcattagc ccggttttca gatactcaat
     1921 accaaagaat tgaaaactac atgaaatcat cggaatttga aaacaagcaa gctctcctga
     1981 aaagagccaa agaggaagta ggtctcctta gggaacataa aattcagaca aacagataca
     2041 cagtaaaggt tcagcgagag ctggagttgg atgaattagc cctgcgtgca ctgaaagagg
     2101 atcgtaaacg cttcttatgt aaagcagttg aaaattatat caactgctta ttaagtggag
     2161 aagaacatga tatgtgggta ttccgacttt gttccctctg gcttgaaaat tctggagttt
     2221 ctgaagtcaa tggcatgatg aagagagacg gaatgaagat tccaacatat aaatttttgc
     2281 ctcttatgta ccaattggct gctagaatgg ggaccaagat gatgggaggc ctaggatttc
     2341 atgaagtcct caataatcta atctctagaa tttcaatgga tcacccccat cacactttgt
     2401 ttattatact ggccttagca aatgcaaaca gagatgaatt tctgactaaa ccagaggtag
     2461 ccagaagaag cagaataact aaaaatgtgc ctaaacaaag ctctcagctt gatgaggatc
     2521 gaacagaggc tgcaaataga ataatatgta ctatcagaag taggagacct cagatggtca
     2581 gaagtgttga ggcactttgt gatgcttata ttatattagc aaacttagat gccactcagt
     2641 ggaagactca gagaaaaggc ataaatattc cagcagacca gccaattact aaacttaaga
     2701 atttagaaga tgttgttgtc cctactatgg aaattaaggt ggaccacaca ggagaatatg
     2761 gaaatctggt gactatacag tcatttaaag cagaatttcg cttagcagga ggtgtaaatt
     2821 taccaaaaat aatagattgt gtaggttccg atggcaagga gaggagacag cttgttaagg
     2881 gccgtgatga cctgagacaa gatgctgtca tgcaacaggt cttccagatg tgtaatacat
     2941 tactgcagag aaacacggaa actaggaaga ggaaattaac tatctgtact tataaggtgg
     3001 ttcccctctc tcagcgaagt ggtgttcttg aatggtgcac aggaactgtc cccattggtg
     3061 aatttcttgt taacaatgaa gatggtgctc ataaaagata caggccaaat gatttcagtg
     3121 cctttcagtg ccaaaagaaa atgatggagg tgcaaaaaaa gtcttttgaa gagaaatatg
     3181 aagtcttcat ggatgtttgc caaaattttc aaccagtttt ccgttacttc tgcatggaaa
     3241 aattcttgga tccagctatt tggtttgaga agcgattggc ttatacgcgc agtgtagcta
     3301 cttcttctat tgttggttac atacttggac ttggtgatag acatgtacag aatatcttga
     3361 taaatgagca gtcagcagaa cttgtacata tagatctagg tgttgctttt gaacagggca
     3421 aaatccttcc tactcctgag acagttcctt ttagactcac cagagatatt gtggatggca
     3481 tgggcattac gggtgttgaa ggtgtcttca gaagatgctg tgagaaaacc atggaagtga
     3541 tgagaaactc tcaggaaact ctgttaacca ttgtagaggt ccttctatat gatccactct
     3601 ttgactggac catgaatcct ttgaaagctt tgtatttaca gcagaggccg gaagatgaaa
     3661 ctgagcttca ccctactctg aatgcagatg accaagaatg caaacgaaat ctcagtgata
     3721 ttgaccagag tttcaacaaa gtagctgaac gtgtcttaat gagactacaa gagaaactga
     3781 aaggagtgga agaaggcact gtgctcagtg ttggtggaca agtgaatttg ctcatacagc
     3841 aggccataga ccccaaaaat ctcagccgac ttttcccagg atggaaagct tgggtgtgta
     3901 cagactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     3961 atgacaagta actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4021 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4081 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt
     4141 tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4201 gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt
     4261 tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4321 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4381 gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg
     4441 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     4501 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     4561 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     4621 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     4681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
     4741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4801 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4861 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4981 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     5041 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5101 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5161 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5221 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5281 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5341 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5401 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5461 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5521 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5581 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5641 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5701 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5761 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5821 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5881 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5941 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6001 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6061 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6121 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6181 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6241 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6301 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6361 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6421 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6481 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6541 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6601 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6661 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6721 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6781 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6841 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6901 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6961 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7021 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7081 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7141 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//