| 质粒名称: | pAAVLINK-EF1a-HA-5'-BCOR |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001662 |
| 产品用途: | 5' AAVLINK plasmid for BCOR expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BCOR |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-BCOR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-BCOR载体质粒基本信息
pAAVLINK-EF1a-HA-5'-BCOR质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-BCOR载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-BCOR质粒载体简介
pAAVLINK-EF1a-HA-5'-BCOR质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-BCOR 6705 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6705)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6705
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3845
/codon_start=1
/label=BCOR
/translation="MLSATPLYGNVHSWMNSERVRMCGASEDRKILVNDGDASKARLEL
REENPLNHNVVDASTAHRIDGLAALSMDRTGLIREGLRVPGNIVYSSLCGLGSEKGREA
ATSTLGGLGFSSERNPEMQFKPNTPETVEASAVSGKPPNGFSAIYKTPPGIQKSAVATA
EALGLDRPASDKQSPLNINGASYLRLPWVNPYMEGATPAIYPFLDSPNKYSLNMYKALL
PQQSYSLAQPLYSPVCTNGERFLYLPPPHYVGPHIPSSLASPMRLSTPSASPAIPPLVH
CADKSLPWKMGVSPGNPVDSHAYPHIQNSKQPRVPSAKAVTSGLPGDTALLLPPSPRPS
PRVHLPTQPAADTYSEFHKHYARISTSPSVALSKPYMTVSSEFPAARLSNGKYPKAPEG
GEGAQPVPGHARKTAVQDRKDGSSPPLLEKQTVTKDVTDKPLDLSSKVVDVDASKADHM
KKMAPTVLVHSRAGSGLVLSGSEIPKETLSPPGNGCAIYRSEIISTAPSSWVVPGPSPN
EENNGKSMSLKNKALDWAIPQQRSSSCPRMGGTDAVITNVSGSVSSAGRPASASPAPNA
NADGTKTSRSSVETTPSVIQHVGQPPATPAKHSSSTSSKGAKASNPEPSFKANENGLPP
SSIFLSPNEAFRSPPIPYPRSYLPYPAPEGIAVSPLSLHGKGPVYPHPVLLPNGSLFPG
HLAPKPGLPYGLPTGRPEFVTYQDALGLGMVHPMLIPHTPIEITKEEKPERRSRSHERA
RYEDPTLRNRFSEILETSSTKLHPDVPTDKNLKPNPNWNQGKTVVKSDKLVYVDLLREE
PDAKTDTNVSKPSFAAESVGQSAEPPKPSVEPALQQHRDFIALREELGRISDFHETYTF
KQPVFTVSKDSVLAGTNKENLGLPVSTPFLEPPLGSDGPAVTFGKTQEDPKPFCVGSAP
PSVDVTPTYTKDGADEAESNDGKVLKPKPSKLAKRIANSAGYVGDRFKCVTTELYADSS
QLSREQRALQMEGLQEDSILCLPAAYCERAMMRFSELEMKERE"
misc_feature 2018..2023
/label=SDS
RBS 2963..2974
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
intron 3846..3886
/label=Splicing Donor
misc_feature 3890..3923
/label=lox2272-JT1571
repeat_region 3968..4108
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3968..4097
/label=AAV2 ITR
rep_origin 4183..4638
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4920..5024
/gene="bla"
/label=AmpR promoter
CDS 5025..5885
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6056..6644
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gctctcagca acccccctgt atgggaacgt
781 tcacagctgg atgaacagcg agagggtccg catgtgtggg gcgagcgaag acaggaaaat
841 ccttgtaaat gatggtgacg cttcaaaagc cagactggaa ctgagggaag agaatccctt
901 gaaccacaac gtggtggatg cgagcacggc ccataggatc gatggcctgg cagcactgag
961 catggaccgc actggcctga tccgggaagg gctgcgggtc ccgggaaaca tcgtctattc
1021 tagcttgtgt ggactgggct cagagaaagg tcgggaggct gccacaagca ctctaggtgg
1081 ccttgggttt tcttcggaaa gaaatccaga gatgcagttc aaaccgaata cacccgagac
1141 agtggaggct tctgccgtct ctggaaaacc cccaaatggc ttcagtgcta tatacaaaac
1201 accgcctgga atacaaaaaa gtgctgtagc cacagcagaa gcgctgggct tggacaggcc
1261 tgccagcgac aaacagagcc ctctcaacat caatggtgct agttatctgc ggctgccctg
1321 ggtcaatcct tacatggagg gtgccacgcc agccatctac cctttcctcg actcgccaaa
1381 taagtattca ctgaacatgt acaaggcctt gctacctcag cagtcctaca gcttggccca
1441 gccgctgtat tctccagtct gcaccaatgg ggagcgcttt ctctacctgc cgccacctca
1501 ctacgtcggt ccccacatcc catcgtcctt ggcatcaccc atgaggctct cgacaccttc
1561 ggcctcccca gccatcccgc ctctcgtcca ttgcgcagac aaaagcctcc cgtggaagat
1621 gggcgtcagc cctgggaatc ctgttgattc ccacgcctat cctcacatcc agaacagtaa
1681 gcagcccagg gttccctctg ccaaggcggt caccagtggc ctgccggggg acacagctct
1741 cctgttgccc ccctcgcctc ggccgtcacc ccgagtccac cttcccaccc agcctgctgc
1801 agacacctac tcggagttcc acaagcacta tgccaggatc tccacctctc cttcagttgc
1861 cctgtcaaag ccatacatga cagttagcag cgagttcccc gcggccaggc tctccaatgg
1921 caagtatccc aaggctccgg aagggggcga aggtgcccag ccagtgcccg ggcatgcccg
1981 gaagacagcg gttcaagaca gaaaagatgg cagctcacct cctctgttgg agaagcagac
2041 cgttaccaaa gacgtcacag ataagccact agacttgtct tctaaagtgg tggatgtaga
2101 tgcttccaaa gctgaccaca tgaaaaagat ggctcccacg gtcctggttc acagcagggc
2161 tggaagtggc ttagtgctct ccggaagtga gattccgaaa gaaacactat ctcctccagg
2221 aaatggttgt gctatctata gatctgaaat catcagcact gctccctcat cctgggtggt
2281 gcccgggcca agtcctaacg aagagaacaa tggcaaaagc atgtcgctga aaaacaaggc
2341 attggactgg gcgataccac agcagcggag ttcatcatgc ccgcgcatgg gcggcaccga
2401 tgctgtcatc actaacgttt cagggtcagt gtcgagtgca ggccgcccag cctccgcatc
2461 acccgccccc aatgccaatg cagatggcac caaaaccagc aggagctctg tagaaaccac
2521 accatccgtt attcagcacg tgggccagcc cccggccact cctgccaagc acagtagcag
2581 caccagcagc aagggcgcca aagccagcaa cccagaaccg agtttcaaag caaacgagaa
2641 cggccttcca ccaagctcta tatttctgtc tccaaatgag gcattcaggt ccccaccaat
2701 tccctacccc aggagttacc tcccttaccc agcccctgag ggcattgctg taagtcccct
2761 ctccttacat ggcaaaggac ctgtctaccc tcacccagtt ttgttaccca atggcagtct
2821 gtttcctggg caccttgccc caaagcctgg gctgccctat gggcttccca ccggccgtcc
2881 agagtttgtg acctaccaag atgccctggg gttgggcatg gtgcatccca tgttgatacc
2941 acacacgccc atagagatta ctaaagagga gaaaccagag aggagatccc ggtcccatga
3001 gagagcccgt tacgaggacc caaccctccg gaatcggttt tccgagattt tggaaactag
3061 cagcaccaag ttacatccag atgtccccac cgacaagaac ctaaagccga accccaactg
3121 gaatcaaggg aagactgttg tcaaaagcga caagcttgtc tacgtagacc ttctccgaga
3181 agaaccagat gctaaaactg acacaaacgt gtccaaaccc agctttgcag cagagagtgt
3241 tggccagagc gctgagcccc ccaagccctc agttgagccg gccctgcagc agcaccgtga
3301 tttcatcgcc ctgagagagg agttggggcg catcagtgac ttccacgaaa cttatacttt
3361 caaacagcca gtcttcaccg taagcaagga cagtgttctg gcaggtacca acaaagagaa
3421 cctagggttg ccagtctcga ctccattcct ggagccacct ctggggagcg atggccctgc
3481 tgtaactttt ggtaaaaccc aagaggatcc caaaccattt tgtgtgggca gtgccccacc
3541 aagtgtggat gtgaccccca cctataccaa agatggagct gatgaggctg aatcaaatga
3601 tggcaaagtt ctgaaaccga agccatctaa gctggcaaag agaatcgcca actcagcggg
3661 ttacgtgggt gaccgattca aatgtgtcac taccgaactg tatgcagatt ccagtcagct
3721 cagccgggag caacgggcat tgcagatgga aggattacaa gaggacagta ttttatgtct
3781 acccgctgct tactgtgagc gtgcaatgat gcgcttctca gagttggaga tgaaagaaag
3841 agaaggtaag tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta
3901 taggatactt tatacgatgt tattaagtta tggagaccgg tgtggcacgt gcggaccgag
3961 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
4021 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
4081 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
4141 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
4201 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
4261 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
4321 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
4381 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
4441 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
4501 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
4561 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
4621 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
4681 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
4741 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
4801 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
4861 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
4921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
4981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
5041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
5101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
5161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
5221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
5281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
5341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
5401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
5461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
5521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
5581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
5641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
5701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
5761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
5821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
5881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
5941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
6001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
6061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
6121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
6181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
6241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
6301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
6361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
6421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
6481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
6541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
6601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
6661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
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