ML1007-pEF1a-iRFP-TAG-nsP4

LOCUS       ML1007-pEF1a-iRFP-TAG-nsP4              9497 bp DNA     circular SYN 18-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ML1007 ML700 piggbac PEF1A-IRFP-TAG-NSP4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   maliang
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   join(874..886,918..919,921..936)
                     /label=5'TR
                     /note="5'TR"
     misc_feature    887..917
                     /label=31bp spacer
                     /note="31bp spacer"
     enhancer        1112..1343
                     /label=core insul
                     /note="core insul enhancer"
     misc_feature    1394..2692
                     /label=Human EF1a Promoter
                     /note="Human EF1a Promoter"
     promoter        1511..2692
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1741..2683
                     /note="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     regulatory      2758..2767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2764..3711
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent
                     protein (Filonov et al., 2011)"
                     /label=phytochrome-based near-infrared fluorescent
                     pro
                     /note="iRFP"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             3715..3735
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T
                     ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    3742..3750
                     /label=readthrough stop codon
                     /note="readthrough stop codon"
     CDS             3751..4209
                     /codon_start=1
                     /label=Delta-nsP3
                     /note="Delta-nsP3"
                     /translation="VDRRQVVVADVHAVQEPAPVPPPRLKKMARLAAARMQEEPTPPAS
                     TSSADESLHLSFGGVSMSFGSLFDGEMARLAAAQPPASTCPTDVPMSFGSFSDGEIEEL
                     SRRVTESEPVLFGSFEPGEVNSIISSRSAVSFPPRKQRRRRRSRRTEY"
     CDS             4210..6060
                     /codon_start=1
                     /label=nsP4
                     /note="nsP4"
                     /translation="LTGVGGYIFSTDTGPGHLQKKSVLQNQLTEPTLERNVLERIYAPV
                     LDTSKEEQLKLRYQMMPTEANKSRYQSRKVENQKAITTERLLSGLRLYNSATDQPECYK
                     ITYPKPSYSSSVPANYSDPKFAVAVCNNYLHENYPTVASYQITDEYDAYLDMVDGTVAC
                     LDTATFCPAKLRSYPKRHEYRAPNIRSAVPSAMQNTLQNVLIAATKRNCNVTQMRELPT
                     LDSATFNVECFRKYACNDEYWEEFARKPIRITTEFVTAYVARLKGPKAAALFAKTHNLV
                     PLQEVPMDRFVMDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLT
                     AVLLPNIHTLFDMSAEDFDAIIAEHFKQGDPVLETDIASFDKSQDDAMALTGLMILEDL
                     GVDQPLLDLIECAFGEISSTHLPTGTRFKFGAMMKSGMFLTLFVNTVLNVVIASRVLEE
                     RLKTSKCAAFIGDDNIIHGVVSDKEMAERCATWLNMEVKIIDAVIGERPPYFCGGFILQ
                     DSVTSTACRVADPLKRLFKLGKPLPADDEQDEDRRRALLDETKAWFRVGITDTLAVAVA
                     TRYEVDNITPVLLALRTFAQSKRAFQAIRGEIKHLYGGPK"
     polyA_signal    6067..6188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        complement(6446..6676)
                     /label=core insul(1)
                     /note="core insul"
     repeat_region   6951..6985
                     /label=3'TR
                     /note="3'TR"
     primer_bind     complement(7476..7492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7500..7516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7524..7554)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7569..7590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7696..8636
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(7878..8466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8637..9497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtgaggccct ttcgtcttca
     1381 agaattctct agaggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga
     1441 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag
     1501 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     1561 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     1621 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     1681 ataagtgcag tagtcgccgt gaacgttcat tttcgcaacg ggtttgccgc cagaacacag
     1741 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     1801 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     1861 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     1921 agttgaggcc tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     1981 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     2041 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     2101 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     2161 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     2221 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     2281 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     2341 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     2401 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     2461 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     2521 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     2581 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     2641 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gagagctcgt
     2701 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct caagcttgcc
     2761 accatggctg aaggatctgt cgccaggcag cctgacctct tgacctgcga cgatgagccg
     2821 atccatatcc ccggtgccat ccaaccgcat ggactgctgc tcgccctcgc cgccgacatg
     2881 acgatcgttg ccggcagcga caaccttccc gaactcaccg gactggcgat cggcgccctg
     2941 atcggccgct ctgcggccga tgtcttcgac tcggagacgc acaaccgtct gacgatcgcc
     3001 ttggccgagc ccggggcggc cgtcggagca ccgatcactg tcggcttcac gatgcgaaag
     3061 gacgcaggct tcatcggctc ctggcatcgc catgatcagc tcatcttcct cgagctcgag
     3121 cctccccagc gggacgtcgc cgagccgcag gcgttcttcc gccgcaccaa cagcgccatc
     3181 cgccgcctgc aggccgccga aaccttggaa agcgcctgcg ccgccgcggc gcaagaggtg
     3241 cggaagatta ccggcttcga tcgggtgatg atctatcgct tcgcctccga cttcagcggc
     3301 gaagtgatcg cagaggatcg gtgcgccgag gtcgagtcaa aactaggcct gcactatcct
     3361 gcctcaaccg tgccggcgca ggcccgtcgg ctctatacca tcaacccggt acggatcatt
     3421 cccgatatca attatcggcc ggtgccggtc accccagacc tcaatccggt caccgggcgg
     3481 ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc ccgtccatct ggaattcatg
     3541 cgcaacatag gcatgcacgg cacgatgtcg atctcgattt tgcgcggcga gcgactgtgg
     3601 ggattgatcg tttgccatca ccgaacgccg tactacgtcg atctcgatgg ccgccaagcc
     3661 tgcgagctag tcgcccaggt tctggcctgg cagatcggcg tgatggaaga gtctccaaaa
     3721 aagaagagaa aggtagtcga ccaatagcaa gtagaccgaa ggcaggtggt ggtggctgac
     3781 gtccatgccg tccaagagcc tgcccctgtt ccaccgccaa ggctaaagaa gatggcccgc
     3841 ctggcagcgg caagaatgca ggaagagcca actccaccgg caagcaccag ctctgcggac
     3901 gagtcccttc acctttcttt tggtggggta tccatgtcct tcggatccct tttcgacgga
     3961 gagatggccc gcttggcagc ggcacaaccc ccggcaagta catgccctac ggatgtgcct
     4021 atgtctttcg gatcgttttc cgacggagag attgaggagc tgagccgcag agtaaccgag
     4081 tctgagcccg tcctgtttgg gtcatttgaa ccgggcgaag tgaactcaat tatatcgtcc
     4141 cgatcagccg tatcttttcc accacgcaag cagagacgta gacgcaggag caggaggacc
     4201 gaatactgac taaccggggt aggtgggtac atattttcga cggacacagg ccctgggcac
     4261 ttgcaaaaga agtccgttct gcagaaccag cttacagaac cgaccttgga gcgcaatgtt
     4321 ctggaaagaa tctacgcccc ggtgctcgac acgtcgaaag aggaacagct caaactcagg
     4381 taccagatga tgcccaccga agccaacaaa agcaggtacc agtccagaaa agtagaaaat
     4441 cagaaagcca taaccactga gcgactgctt tcagggctac gactgtataa ctctgccaca
     4501 gatcagccag aatgctataa gatcacctac ccgaaaccat cgtattccag cagtgtaccg
     4561 gcgaactact ctgacccaaa gtttgctgta gctgtttgca acaactatct gcatgagaat
     4621 tacccgacgg tagcatctta tcagatcacc gacgagtacg atgcttactt ggatatggta
     4681 gacgggacag tcgcttgcct agatactgca actttttgcc ccgccaagct tagaagttac
     4741 ccgaaaagac acgagtatag agccccaaac atccgcagtg cggttccatc agcgatgcag
     4801 aacacgttgc aaaacgtgct cattgccgcg actaaaagaa actgcaacgt cacacaaatg
     4861 cgtgaattgc caacactgga ctcagcgaca ttcaacgttg aatgctttcg aaaatatgca
     4921 tgtaatgacg agtattggga ggagtttgcc cgaaagccaa ttaggatcac tactgagttc
     4981 gttaccgcat acgtggccag actgaaaggc cctaaggccg ccgcactgtt cgcaaagacg
     5041 cataatttgg tcccattgca agaagtgcct atggataggt tcgtcatgga catgaaaaga
     5101 gacgtgaaag ttacacctgg cacgaaacac acagaagaaa gaccgaaagt acaagtgata
     5161 caagccgcag aacccctggc gaccgcttac ctgtgcggga tccaccggga gttagtgcgc
     5221 aggcttacag ccgtcttgct acccaacatt cacacgcttt ttgacatgtc ggcggaggac
     5281 tttgatgcaa tcatagcaga acacttcaag caaggtgacc cggtactgga gacggatatc
     5341 gcctcgttcg acaaaagcca agacgacgct atggcgttaa ctggcctgat gatcttggaa
     5401 gacctgggtg tggaccaacc actactcgac ttgatcgagt gcgcctttgg agaaatatca
     5461 tccacccatc tgcccacggg tacccgtttc aaattcgggg cgatgatgaa atccggaatg
     5521 ttcctcacgc tctttgtcaa cacagttctg aatgtcgtta tcgccagcag agtattggag
     5581 gagcggctta aaacgtccaa atgtgcagca tttatcggcg acgacaacat catacacgga
     5641 gtagtatctg acaaagaaat ggctgagagg tgtgccacct ggctcaacat ggaggttaag
     5701 atcattgacg cagtcatcgg cgagagaccg ccttacttct gcggtggatt catcttgcaa
     5761 gattcggtta cctccacagc gtgtcgcgtg gcggacccct tgaaaaggct gtttaagttg
     5821 ggtaaaccgc tcccagccga cgatgagcaa gacgaagaca gaagacgcgc tctgctagat
     5881 gaaacaaagg cgtggtttag agtaggtata acagacacct tagcagtggc cgtggcaact
     5941 cggtatgagg tagacaacat cacacctgtc ctgctggcat tgagaacttt tgcccagagc
     6001 aaaagagcat ttcaagccat cagaggggaa ataaagcatc tctacggtgg tcctaaatag
     6061 taactaaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6181 gtatcttatc atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc
     6241 tggtctccct tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa
     6301 actgggttct tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag
     6361 gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaattg actggattcc
     6421 ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca
     6481 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
     6541 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
     6601 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
     6661 ggggggggct gtccctgata tctataacaa gaaaatatat atataataag ttatcacgta
     6721 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     6781 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     6841 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     6901 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     6961 ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc
     7021 ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc
     7081 ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg
     7141 ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat
     7201 gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct
     7261 tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg
     7321 cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata
     7381 ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg
     7441 tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7501 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7561 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7621 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7681 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7741 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7801 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7861 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7921 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7981 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8041 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8101 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8161 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8221 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8281 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8341 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8401 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8461 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8521 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8581 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8641 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8701 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8761 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8821 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8881 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8941 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9001 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9061 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9121 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9181 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9241 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9301 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9361 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9421 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9481 aaatgttgaa tactcat
//