ML700 pEF1a-iRFP-nsP4 for high-replicase host cells

LOCUS       ML700 pEF1a-iRFP-nsP4 for high-replicase host cells             9539 bp DNA     circular SYN 18-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   maliang
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   join(874..886,918..919,921..936)
                     /label=5'TR
                     /note="5'TR"
     misc_feature    887..917
                     /label=31bp spacer
                     /note="31bp spacer"
     enhancer        1112..1343
                     /label=core insul
                     /note="core insul enhancer"
     misc_feature    1394..2692
                     /label=Human EF1a Promoter
                     /note="Human EF1a Promoter"
     promoter        1511..2692
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1741..2683
                     /note="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     regulatory      2758..2767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2764..3711
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent
                     protein (Filonov et al., 2011)"
                     /label=phytochrome-based near-infrared fluorescent
                     pro
                     /note="iRFP"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             3715..3735
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T
                     ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             3736..3792
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=2A peptide from porcine teschovirus-1
                     polyprotein
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3793..4251
                     /codon_start=1
                     /label=Delta-nsP3
                     /note="Delta-nsP3"
                     /translation="VDRRQVVVADVHAVQEPAPVPPPRLKKMARLAAARMQEEPTPPAS
                     TSSADESLHLSFGGVSMSFGSLFDGEMARLAAAQPPASTCPTDVPMSFGSFSDGEIEEL
                     SRRVTESEPVLFGSFEPGEVNSIISSRSAVSFPPRKQRRRRRSRRTEY"
     CDS             4252..6102
                     /codon_start=1
                     /label=nsP4
                     /note="nsP4"
                     /translation="LTGVGGYIFSTDTGPGHLQKKSVLQNQLTEPTLERNVLERIYAPV
                     LDTSKEEQLKLRYQMMPTEANKSRYQSRKVENQKAITTERLLSGLRLYNSATDQPECYK
                     ITYPKPSYSSSVPANYSDPKFAVAVCNNYLHENYPTVASYQITDEYDAYLDMVDGTVAC
                     LDTATFCPAKLRSYPKRHEYRAPNIRSAVPSAMQNTLQNVLIAATKRNCNVTQMRELPT
                     LDSATFNVECFRKYACNDEYWEEFARKPIRITTEFVTAYVARLKGPKAAALFAKTHNLV
                     PLQEVPMDRFVMDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLT
                     AVLLPNIHTLFDMSAEDFDAIIAEHFKQGDPVLETDIASFDKSQDDAMALTGLMILEDL
                     GVDQPLLDLIECAFGEISSTHLPTGTRFKFGAMMKSGMFLTLFVNTVLNVVIASRVLEE
                     RLKTSKCAAFIGDDNIIHGVVSDKEMAERCATWLNMEVKIIDAVIGERPPYFCGGFILQ
                     DSVTSTACRVADPLKRLFKLGKPLPADDEQDEDRRRALLDETKAWFRVGITDTLAVAVA
                     TRYEVDNITPVLLALRTFAQSKRAFQAIRGEIKHLYGGPK"
     polyA_signal    6109..6230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        complement(6488..6718)
                     /label=core insul(1)
                     /note="core insul"
     repeat_region   6993..7027
                     /label=3'TR
                     /note="3'TR"
     primer_bind     complement(7518..7534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7542..7558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7566..7596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7611..7632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7738..8678
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(7920..8508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8679..9539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtgaggccct ttcgtcttca
     1381 agaattctct agaggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga
     1441 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag
     1501 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     1561 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     1621 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     1681 ataagtgcag tagtcgccgt gaacgttcat tttcgcaacg ggtttgccgc cagaacacag
     1741 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     1801 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     1861 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     1921 agttgaggcc tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     1981 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     2041 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     2101 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     2161 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     2221 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     2281 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     2341 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     2401 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     2461 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     2521 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     2581 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     2641 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gagagctcgt
     2701 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct caagcttgcc
     2761 accatggctg aaggatctgt cgccaggcag cctgacctct tgacctgcga cgatgagccg
     2821 atccatatcc ccggtgccat ccaaccgcat ggactgctgc tcgccctcgc cgccgacatg
     2881 acgatcgttg ccggcagcga caaccttccc gaactcaccg gactggcgat cggcgccctg
     2941 atcggccgct ctgcggccga tgtcttcgac tcggagacgc acaaccgtct gacgatcgcc
     3001 ttggccgagc ccggggcggc cgtcggagca ccgatcactg tcggcttcac gatgcgaaag
     3061 gacgcaggct tcatcggctc ctggcatcgc catgatcagc tcatcttcct cgagctcgag
     3121 cctccccagc gggacgtcgc cgagccgcag gcgttcttcc gccgcaccaa cagcgccatc
     3181 cgccgcctgc aggccgccga aaccttggaa agcgcctgcg ccgccgcggc gcaagaggtg
     3241 cggaagatta ccggcttcga tcgggtgatg atctatcgct tcgcctccga cttcagcggc
     3301 gaagtgatcg cagaggatcg gtgcgccgag gtcgagtcaa aactaggcct gcactatcct
     3361 gcctcaaccg tgccggcgca ggcccgtcgg ctctatacca tcaacccggt acggatcatt
     3421 cccgatatca attatcggcc ggtgccggtc accccagacc tcaatccggt caccgggcgg
     3481 ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc ccgtccatct ggaattcatg
     3541 cgcaacatag gcatgcacgg cacgatgtcg atctcgattt tgcgcggcga gcgactgtgg
     3601 ggattgatcg tttgccatca ccgaacgccg tactacgtcg atctcgatgg ccgccaagcc
     3661 tgcgagctag tcgcccaggt tctggcctgg cagatcggcg tgatggaaga gtctccaaaa
     3721 aagaagagaa aggtagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
     3781 aaccctggac ctgtagaccg aaggcaggtg gtggtggctg acgtccatgc cgtccaagag
     3841 cctgcccctg ttccaccgcc aaggctaaag aagatggccc gcctggcagc ggcaagaatg
     3901 caggaagagc caactccacc ggcaagcacc agctctgcgg acgagtccct tcacctttct
     3961 tttggtgggg tatccatgtc cttcggatcc cttttcgacg gagagatggc ccgcttggca
     4021 gcggcacaac ccccggcaag tacatgccct acggatgtgc ctatgtcttt cggatcgttt
     4081 tccgacggag agattgagga gctgagccgc agagtaaccg agtctgagcc cgtcctgttt
     4141 gggtcatttg aaccgggcga agtgaactca attatatcgt cccgatcagc cgtatctttt
     4201 ccaccacgca agcagagacg tagacgcagg agcaggagga ccgaatactg actaaccggg
     4261 gtaggtgggt acatattttc gacggacaca ggccctgggc acttgcaaaa gaagtccgtt
     4321 ctgcagaacc agcttacaga accgaccttg gagcgcaatg ttctggaaag aatctacgcc
     4381 ccggtgctcg acacgtcgaa agaggaacag ctcaaactca ggtaccagat gatgcccacc
     4441 gaagccaaca aaagcaggta ccagtccaga aaagtagaaa atcagaaagc cataaccact
     4501 gagcgactgc tttcagggct acgactgtat aactctgcca cagatcagcc agaatgctat
     4561 aagatcacct acccgaaacc atcgtattcc agcagtgtac cggcgaacta ctctgaccca
     4621 aagtttgctg tagctgtttg caacaactat ctgcatgaga attacccgac ggtagcatct
     4681 tatcagatca ccgacgagta cgatgcttac ttggatatgg tagacgggac agtcgcttgc
     4741 ctagatactg caactttttg ccccgccaag cttagaagtt acccgaaaag acacgagtat
     4801 agagccccaa acatccgcag tgcggttcca tcagcgatgc agaacacgtt gcaaaacgtg
     4861 ctcattgccg cgactaaaag aaactgcaac gtcacacaaa tgcgtgaatt gccaacactg
     4921 gactcagcga cattcaacgt tgaatgcttt cgaaaatatg catgtaatga cgagtattgg
     4981 gaggagtttg cccgaaagcc aattaggatc actactgagt tcgttaccgc atacgtggcc
     5041 agactgaaag gccctaaggc cgccgcactg ttcgcaaaga cgcataattt ggtcccattg
     5101 caagaagtgc ctatggatag gttcgtcatg gacatgaaaa gagacgtgaa agttacacct
     5161 ggcacgaaac acacagaaga aagaccgaaa gtacaagtga tacaagccgc agaacccctg
     5221 gcgaccgctt acctgtgcgg gatccaccgg gagttagtgc gcaggcttac agccgtcttg
     5281 ctacccaaca ttcacacgct ttttgacatg tcggcggagg actttgatgc aatcatagca
     5341 gaacacttca agcaaggtga cccggtactg gagacggata tcgcctcgtt cgacaaaagc
     5401 caagacgacg ctatggcgtt aactggcctg atgatcttgg aagacctggg tgtggaccaa
     5461 ccactactcg acttgatcga gtgcgccttt ggagaaatat catccaccca tctgcccacg
     5521 ggtacccgtt tcaaattcgg ggcgatgatg aaatccggaa tgttcctcac gctctttgtc
     5581 aacacagttc tgaatgtcgt tatcgccagc agagtattgg aggagcggct taaaacgtcc
     5641 aaatgtgcag catttatcgg cgacgacaac atcatacacg gagtagtatc tgacaaagaa
     5701 atggctgaga ggtgtgccac ctggctcaac atggaggtta agatcattga cgcagtcatc
     5761 ggcgagagac cgccttactt ctgcggtgga ttcatcttgc aagattcggt tacctccaca
     5821 gcgtgtcgcg tggcggaccc cttgaaaagg ctgtttaagt tgggtaaacc gctcccagcc
     5881 gacgatgagc aagacgaaga cagaagacgc gctctgctag atgaaacaaa ggcgtggttt
     5941 agagtaggta taacagacac cttagcagtg gccgtggcaa ctcggtatga ggtagacaac
     6001 atcacacctg tcctgctggc attgagaact tttgcccaga gcaaaagagc atttcaagcc
     6061 atcagagggg aaataaagca tctctacggt ggtcctaaat agtaactaaa cttgtttatt
     6121 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6181 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6241 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     6301 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     6361 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     6421 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     6481 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     6541 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     6601 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     6661 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     6721 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     6781 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     6841 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     6901 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     6961 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     7021 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     7081 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     7141 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     7201 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     7261 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     7321 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     7381 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     7441 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     7501 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7561 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7621 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7681 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7741 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7801 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7861 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7921 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8101 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8221 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8281 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8341 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8401 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8461 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8521 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8581 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8641 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8701 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8761 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8821 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8881 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8941 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9001 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9061 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9301 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9361 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9421 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9481 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcat
//