pDHt/sk-PC

LOCUS       pDHt/sk-PC               15460 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma
            reesei .
ACCESSION   .
VERSION     .
KEYWORDS    pDHt/sk-PC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15460)
  AUTHORS   Liu R, Chen L, Jiang Y, Zhou Z, Zou G
  TITLE     Efficient genome editing in filamentous fungus Trichoderma reesei 
            using the CRISPR/Cas9 system.
  JOURNAL   Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. 
            eCollection 2015.
  PUBMED    27462408
REFERENCE   2  (bases 1 to 15460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15460
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     3..23
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5..23
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             427..1452
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIDKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1960..2134
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2212..2236
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2661..3455
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3542..4130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4031..4050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4284..4301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4316..4456
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4382..4401)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4542..4564
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4800..4994
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5060..6133)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6562..7191)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8492..8516
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8704..8726
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8718..8735
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8719..8735
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10279..14382
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             14395..14415
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcg
       61 acgttaactg atattgaagg agcacttttt gggcttggct ggagctagtg gaggtcaaca
      121 atgaatgcct attttggttt agtcgtccag gcggtgagca caaaatttgt gtcgtttgac
      181 aagatggttc atttaggcaa ctggtcagat cagccccact tgtagcagta gcggcggcgc
      241 tcgaagtgtg actcttatta gcagacagga actaggacat tatcatcatc tgctgcttgg
      301 tgcacgataa cttggtgcgt ttgtcaagca aggtaagtga acgacccggt cataccttct
      361 taagttcgcc cttcctccct ttgtttcaga ttcaatctga cttacctatt ctacccaagc
      421 atcgatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgacaag
      481 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
      541 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
      601 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
      661 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
      721 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
      781 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
      841 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
      901 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
      961 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     1021 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     1081 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     1141 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     1201 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     1261 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     1321 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     1381 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     1441 gcaaaggaat agagtagatg ccgaccggga tcgatccact taacgttact gaaatcatca
     1501 aacagcttga cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga
     1561 caaatggtgt tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct
     1621 tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgtttcgggt ttacctcttc
     1681 cagatacagc tcaactgcaa tgcattaatg cattggacct cgcaacccta gtacgccctt
     1741 caggctccgg cgaagcagaa gaatagctta gcagagtcta ttttcatttt cgggagacga
     1801 gatcaagcag atcaacggtc gtcaagagac ctacgagact gaggaatccg ctcttggctc
     1861 cacgcgacta tatatttgtc tctaattgta ctttgacatg ctcctcttct ttactctgat
     1921 agcttgacta tgaaaattcc gtcaccagcc ctgctcgagt ttctccataa taatgtgtga
     1981 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     2041 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     2101 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
     2161 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     2221 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     2281 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     2341 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     2401 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     2461 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
     2521 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
     2581 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
     2641 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
     2701 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
     2761 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
     2821 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
     2881 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     2941 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     3001 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     3061 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
     3121 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
     3181 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     3241 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     3301 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     3361 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     3421 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
     3481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3661 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     4321 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     4381 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     4441 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4501 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4561 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     4621 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     4681 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     4741 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     4801 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     4861 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     4921 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     4981 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     5041 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     5101 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     5161 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     5221 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     5281 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     5341 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     5401 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     5461 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     5521 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     5581 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     5641 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     5701 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     5761 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     5821 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     5881 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     5941 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     6001 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     6061 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     6121 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     6181 ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
     6241 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
     6301 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
     6361 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
     6421 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
     6481 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
     6541 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
     6601 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
     6661 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
     6721 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
     6781 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
     6841 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
     6901 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
     6961 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
     7021 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
     7081 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
     7141 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
     7201 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
     7261 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
     7321 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
     7381 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
     7441 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
     7501 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
     7561 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
     7621 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
     7681 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
     7741 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
     7801 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
     7861 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
     7921 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
     7981 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
     8041 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
     8101 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
     8161 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
     8221 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
     8281 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
     8341 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
     8401 gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc
     8461 gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca
     8521 ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc
     8581 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8641 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8701 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttaggact tccagggcta
     8761 cttggcgcgg cagaagccgc aggtgtcgag ccgggaggag ttttcgcagt ggctgtgccg
     8821 cgcgcacaac gatgtcaacc ggaagctggg caagcccgag tttgactgct cgcgggtgga
     8881 cgagcggtgg aggacggggt ggaaggacgg tcggtgcgac tgattggcca tgaattccat
     8941 ctttcgagga cggacgaaga tactgtacga ttaatgaaag gagggagcat tcttcgactt
     9001 gcggcaattg catgcacatg tacgattgga agcgcgggcg atgtattcgc aatcatgttt
     9061 agaaggacgg cgtttggaaa cgttgggatg ctgttgaagc gttggaaaca ggggcaatta
     9121 gaaacaccga gccagacaga gtcaatggta cgaggtcagc cagtatcatg acctgtgtgc
     9181 gcatggtggc gagagattcc gagccatgcc acgggagacg agcaatgaaa aaactcttca
     9241 ctcacttgtc gaggctctct caacctatcg acttatcaag tagacgatga aagccttgca
     9301 actgtggtga tgtggctcat caatgtgcga cgtcgtatcc atgtctgagg ccattcgata
     9361 tcgtgatgcg actacctagt aaagcccggc cagagggcaa accggggcga caggggcagg
     9421 caattgaccg gatggctgca tgtgccgaag cagccccgat ggaatcgaga tgtctgtcgg
     9481 atggaccgct gagcggcctg gcaaggtgtc ccagatacga agatggaagt gaagtcagag
     9541 gtggtcgtta attgtccgac gagcgaatcg gccgctcctt cggattgccg gctctgctgt
     9601 atgtaccgtg catgaagcca cccgggatcc atgttacgat ggataggttc caactctcta
     9661 gtagctatag tggacctgag gctatctagt atcactggag gagcagccgt ccactatcgt
     9721 cgagcgctgt agaagcagct gcattagcgg ctgcccaccc gcgcagaaat ggccccatta
     9781 catcactatc atgacagcgg cgcgtccaaa agtgagctca tgcttgccga tggcacgagc
     9841 agctgcaact ggcggggctc ctgcctgccg tctccggtgc cgctgcccat ttgagtttgt
     9901 ccgagctgtt gatggttgaa accgagaccg atggatgatt caacacttcg aagtctaggt
     9961 agataaaaaa catctatata tcctcattca ttgccctgtc agtgtgttgg ctcacgtctc
    10021 caatcctccg cccctcctcc tgcaaagtaa ataccttctc aaaacacgtc tggaatcctg
    10081 caagtctcca tcacaaggag cttcttcatc aaccacctta tacgagcaac atcatttgca
    10141 tcatcgttga tccacatctc ctcgcgcctc agagtgtcgt caccagtata aataaccgca
    10201 tcaagctctc gtccttcttc gttccacaat ccaagaagca cctcaaaacg atcaaagcag
    10261 cgcagctaca gcacaatcat ggacaagaag tacagcattg gcctggacat tggcacgaac
    10321 tcggtcggct gggccgtcat cacggacgag tacaaggtcc cctccaagaa gtttaaggtc
    10381 ctgggcaaca ccgaccgcca ctccatcaag aagaacctca ttggcgccct gctcttcgac
    10441 tccggcgaga ccgccgaggc cacccgcctc aagcgcaccg cccgccgccg atacacgcgc
    10501 cgcaagaacc gcatctgcta cctgcaggag attttctcca acgagatggc caaggtcgac
    10561 gactccttct ttcaccgcct ggaggagtcg ttcctcgtcg aggaagacaa gaagcacgag
    10621 cgccacccca tctttggcaa cattgtcgac gaggtcgcct accacgagaa gtaccccacg
    10681 atctaccacc tgcgcaagaa gctcgtcgac tccaccgaca aggccgacct ccgcctgatc
    10741 tacctcgccc tggcccacat gattaagttc cgcggccact ttctgatcga gggcgacctc
    10801 aaccccgaca acagcgacgt cgacaagctg ttcatccagc tcgtccagac ctacaaccag
    10861 ctctttgagg agaaccccat taacgcctcc ggcgtcgacg ccaaggccat cctctcggcc
    10921 cgcctctcca agagccgccg actcgagaac ctgatcgccc agctgcccgg cgagaagaag
    10981 aacggcctgt tcggcaacct catcgccctc tccctgggcc tcacccccaa cttcaagtcg
    11041 aactttgacc tcgccgagga cgccaagctg cagctctcca aggacaccta cgacgacgac
    11101 ctggacaacc tcctggccca gatcggcgac cagtacgccg acctgttcct cgccgccaag
    11161 aacctgtccg acgccatcct cctgtcggac attctccgcg tcaacaccga gattacgaag
    11221 gcccctctct ccgcctcgat gatcaagcgc tacgacgagc accaccagga cctgaccctg
    11281 ctcaaggccc tggtccgcca gcagctcccc gagaagtaca aggagatctt ctttgaccag
    11341 agcaagaacg gctacgccgg ctacatcgac ggcggcgcta gccaagagga gttctacaag
    11401 tttatcaagc ccattctgga gaagatggac ggcacggagg agctcctggt caagctcaac
    11461 cgcgaggacc tcctgcgcaa gcagcgcacc ttcgacaacg gcagcatccc ccaccagatt
    11521 cacctcggcg agctgcacgc catcctccgc cgacaagagg acttctaccc ctttctcaag
    11581 gacaaccgcg agaagatcga gaagattctg acgttccgca tcccctacta cgtcggcccc
    11641 ctggcccgcg gcaacagccg ctttgcctgg atgacccgca agtccgagga gaccatcacg
    11701 ccctggaact tcgaggaagt cgtcgacaag ggcgcctcgg cccagtcctt catcgagcgc
    11761 atgaccaact ttgacaagaa cctgcccaac gagaaggtcc tccccaagca ctcgctcctg
    11821 tacgagtact tcaccgtcta caacgagctc acgaaggtca agtacgtcac cgagggcatg
    11881 cgcaagcccg ccttcctgtc gggcgagcag aagaaggcca tcgtcgacct cctgtttaag
    11941 accaaccgca aggtcacggt caagcagctc aaggaagact acttcaagaa gattgagtgc
    12001 tttgacagcg tcgagatctc cggcgtcgag gaccgcttta acgcctccct gggcacctac
    12061 cacgacctcc tgaagatcat taaggacaag gacttcctgg acaacgagga gaacgaggac
    12121 atcctcgagg acattgtcct gaccctcacg ctgtttgagg accgcgagat gatcgaggag
    12181 cgcctgaaga cgtacgccca cctcttcgac gacaaggtca tgaagcagct caagcgccgc
    12241 cgatacaccg gctggggccg cctgagccgc aagctcatca acggcattcg cgacaagcag
    12301 tcgggcaaga cgatcctcga cttcctgaag agcgacggct tcgccaaccg caactttatg
    12361 cagctgattc acgacgactc cctcaccttc aaggaagaca tccagaaggc ccaggtctcc
    12421 ggccagggcg actccctgca cgagcacatc gccaacctcg ccggcagccc cgccatcaag
    12481 aagggcattc tgcagaccgt caaggtcgtc gacgagctcg tcaaggtcat gggccgccac
    12541 aagcccgaga acatcgtcat tgagatggcc cgcgagaacc agaccacgca gaagggccag
    12601 aagaacagcc gcgagcgcat gaagcgcatc gaggaaggca tcaaggagct gggctcccag
    12661 atcctcaagg agcaccccgt cgagaacacc cagctgcaga acgagaagct ctacctgtac
    12721 tacctccaga acggccgcga catgtacgtc gaccaggagc tggacattaa ccgcctctcg
    12781 gactacgacg tcgaccacat cgtcccccag agcttcctga aggacgactc catcgacaac
    12841 aaggtcctca cccgcagcga caagaaccgc ggcaagagcg acaacgtccc ctccgaggaa
    12901 gtcgtcaaga agatgaagaa ctactggcgc cagctcctga acgccaagct gatcacgcag
    12961 cgcaagtttg acaacctcac caaggccgag cgaggcggcc tctcggagct ggacaaggcc
    13021 ggcttcatca agcgccagct ggtcgagacc cgccagatca cgaagcacgt cgcccagatt
    13081 ctcgactcgc gcatgaacac gaagtacgac gagaacgaca agctgatccg cgaggtcaag
    13141 gtcattaccc tgaagtcgaa gctcgtcagc gacttccgca aggacttcca gttttacaag
    13201 gtccgcgaga tcaacaacta ccaccacgcc cacgacgcct acctcaacgc cgtcgtcggc
    13261 accgccctga tcaagaagta ccccaagctc gagtccgagt tcgtctacgg cgactacaag
    13321 gtctacgacg tccgcaagat gatcgccaag tccgagcagg agattggcaa ggccaccgcc
    13381 aagtacttct tttactcgaa catcatgaac ttctttaaga ccgagatcac cctcgccaac
    13441 ggcgagatcc gcaagcgccc cctcattgag accaacggcg agaccggcga gatcgtctgg
    13501 gacaagggcc gcgacttcgc caccgtccgc aaggtcctca gcatgcccca ggtcaacatc
    13561 gtcaagaaga ccgaggtcca gacgggcggc ttctcgaagg agagcattct gcccaagcgc
    13621 aactccgaca agctcatcgc ccgcaagaag gactgggacc ccaagaagta cggtggcttc
    13681 gactccccca ccgtcgccta ctcggtcctg gtcgtcgcca aggtcgagaa gggcaagtcg
    13741 aagaagctca agagcgtcaa ggagctcctg ggcatcacca ttatggagcg cagctccttc
    13801 gagaagaacc ccatcgactt tctcgaggcc aagggctaca aggaagtcaa gaaggacctg
    13861 atcattaagc tccccaagta ctccctcttc gagctggaga acggccgcaa gcgcatgctc
    13921 gcctccgccg gcgagctcca gaagggcaac gagctcgccc tgcccagcaa gtacgtcaac
    13981 ttcctctacc tggccagcca ctacgagaag ctcaagggct cccccgagga caacgagcag
    14041 aagcagctgt ttgtcgagca gcacaagcac tacctcgacg agatcattga gcagatttcc
    14101 gagttctcga agcgcgtcat cctggccgac gccaacctgg acaaggtcct cagcgcctac
    14161 aacaagcacc gcgacaagcc catccgcgag caggccgaga acatcattca cctcttcacc
    14221 ctgaccaacc tcggcgcccc cgccgccttc aagtactttg acaccacgat cgaccgcaag
    14281 cgctacacct cgacgaagga agtcctggac gccaccctca tccaccagag cattaccggc
    14341 ctctacgaga cgcgcatcga cctcagccag ctcggcggcg actcccgcgc cgaccccaag
    14401 aagaagcgca aggtctaacc cggcatgaag tctgaccggg tagtatgagg gttcatcgtc
    14461 accttgatag aataatagac gataaagcag gccacgggca ggtaccgatt gtcaatccgg
    14521 caggttcgga ggcgtgttgg aaatgagttt atgggttatg gtcaaatcgg atagtatgag
    14581 gtacatagtt tgtaaatctc aagattattt tcttccttaa tcttgcacgt cgcatgagag
    14641 ggaccgagaa gagaattgat gaagggctct tgaagatgag atgaatcacg tggttgctga
    14701 agcttcagta gtctcgggta cctgttcttt cccacaaaca gtagccaggc tagaggtact
    14761 gagtacccgc tcaccgtatc taatcatccg acctgaaatc ttcaagctgt tttattgaca
    14821 cttcgagtcc atcttcattc acgtaaggag aacttctagg acatcactta tcccgccata
    14881 tttagctgca aggagtcaat tgcaatgtca gattccgctc ctaagaggaa acagggccct
    14941 ggcggctcag atggctcggc attgaagaag agaaaggtat gatgacaaga atgcttcttt
    15001 tacatattta cgaagaatgc caccaccctt ctctctggct acctatgtcc atggacaacg
    15061 agcttgctga caatgggact atgaatgggt acctaggagg gcacgggagg aagatggaag
    15121 acgccagctc aaaaggcaaa gcaggcctcg tgggtcgaga tgggcaaggc tctcgaggtg
    15181 ggcgacgaag gcatctgggt cacgtatgcg agaggcatga agaacaaggc cgttcgggag
    15241 tttacggcca tgtgccatga ggtaaagctg cataatccca tgcccaccca cggggagact
    15301 attttagatg tttattggtt gcggcgggga tgataataac gatgttggca gtacggcgaa
    15361 aagctctacg ggatcacgcc cccggccgac gagtcagaag ctcaccaccc ggccgacgga
    15421 gccgaagagg aagacatcga ggcgtccatc tagaggatcc
//