| 质粒名称: | Str-Ii_VSVG-SBP-EGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 65300 |
| 存储实验室: | Franck Perez |
|
载体骨架基本信息 |
|
| 载体名称: | pIRESneo3 |
| 载体出品公司: | Clontech |
| 空载体大小: | 8033bp |
| 完整质粒大小: | 8033bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VSV-G |
| 启动子: | CMV |
| 标签/融合蛋白: |
Streptavidin Binding Protein (SBP) (C terminal on insert)
EGFP (C terminal on insert)
|
| 克隆方法: | 未知 |
| 5’克隆位点: | IRES-F |
| 3’克隆位点: | EGFP-N |
|
文献引用方法 |
|
| 材料和方法部分: | Str-Ii_VSVG-SBP-EGFP was a gift from Franck Perez (Addgene plasmid # 65300 ; http://n2t.net/addgene:65300 ; RRID:Addgene_65300) |
| 参考文献部分: | Synchronization of secretory protein traffic in populations of cells. Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche L, Mercanti V, Jollivet F, Raposo G, Perez F. Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928. 10.1038/nmeth.1928 PubMed 22406856 |
买家导航
Str-Ii_VSVG-SBP-EGFP载体质粒基本信息
Str-Ii_VSVG-SBP-EGFP质粒图谱载体图谱和Str-Ii_VSVG-SBP-EGFP载体序列质粒序列多克隆位点信息
Str-Ii_VSVG-SBP-EGFP质粒载体简介
Str-Ii_VSVG-SBP-EGFP质粒序列载体序列
LOCUS Str-Ii_VSVG-SBP-EGFP 8033 bp DNA circular SYN 15-DEC-2025
DEFINITION synchronize trafficking of VSVG from the ER (RUSH system).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8033)
AUTHORS Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche
L, Mercanti V, Jollivet F, Raposo G, Perez F
TITLE Synchronization of secretory protein traffic in populations of
cells.
JOURNAL Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
PUBMED 22406856
REFERENCE 2 (bases 1 to 8033)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth";
journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9";
issue: "5"; pages: "493-8"
COMMENT Sequence Label: Str-Ii_VSVG-SBP-EGFP
FEATURES Location/Qualifiers
source 1..8033
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(48..67)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 239..618
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..822
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 773..793
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 867..886
/label=T7
/note="T7 promoter, forward primer"
promoter 867..885
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1459..1485
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
intron 2220..2449
/label=chimeric intron
/note="chimera between introns from adenovirus and
immunoglobulin heavy chain genes"
primer_bind 2432..2451
/label=pMT2-F
/note="Synthetic intron, forward primer"
misc_feature join(2522..3096,3097..3099,3100..3108)
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(2689..2706)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2916..2935
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS join(3122..3169,3170..4654)
/codon_start=1
/product="vesicular stomatitis virus G glycoprotein"
/label=VSV-G
/note="Indiana strain"
/translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
CDS 4664..4777
/codon_start=1
/product="streptavidin-binding peptide"
/label=SBP
/note="selected from a peptide library; binds streptavidin
with nanomolar affinity (Keefe et al., 2001)"
/translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
CDS join(4787..4789,4790..4792,4793..5503)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(4787..4789,4790..4792,4793..5503)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4832..4853)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5093..5112)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5440..5461
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 5657..5778
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5694..5713)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(5880..5896)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5880..5896)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5893..5915)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5904..5920
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5928..5934,5935..5952,5953..5958))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5973..5994
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6111..6128)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6282..6870)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6362..6381)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(7041..7832,7833..7901))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7664..7683
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7902..8006)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
841 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
901 taccgagctc ggatcgatat catgcaccgg aggagatcac gctcttgtag ggaggaccag
961 aaacctgtca ccggtgaccc tagcaaagac tcaaaagctc aggtgtccgc tgccgaggct
1021 ggcattactg gaacatggta caatcagctc gggagcacct ttattgtgac tgctggagcc
1081 gatggagccc tcaccggaac atacgaatct gctgtgggaa acgccgaatc acggtacgtc
1141 ctcactggcc gatacgatag tgcccctgcc accgacggat ctgggactgc cctgggatgg
1201 actgtcgctt ggaaaaacaa ctaccggaat gctcattctg ccacaacatg gagtggacag
1261 tacgtgggag gcgctgaggc tagaatcaat acacagtggc tgctcacatc tggcacaacc
1321 gaggcaaatg cttggaaatc caccctggtg ggacatgaca cattcaccaa agtgaaaccc
1381 tccgccgctt caatcgatgc cgccaaaaaa gccggagtca acaacggcaa tcctctggat
1441 gccgtccagc aggtcgacta tccgtacgac gtaccagact acgcagtcgg accgatggac
1501 gatcagaggg acctcattag caacaacgaa cagctgccta tgctgggacg gcgacctgga
1561 gcccctgaat ccaaatgctc taggggagca ctgtacactg gcttctccat tctcgtgaca
1621 ctgctgctgg ccgggcaggc tactactgct tacttcctgt accagcagca ggggcggctg
1681 gacaaactca ctgtgacatc tcagaacctc cagctggaaa atctgaggat gaaactgccc
1741 aaacccccta aacccgtgtc caaaatgagg atggccacac ctctgctcat gcaggcactg
1801 ccaatgggag ccctgcccca ggggcccatg cagaatgcca ccaagtatgg caacatgaca
1861 gaggaccatg tgatgcacct gctccagaat gctgaccccc tgaaggtgta cccgccactg
1921 aaggggagct tcccggagaa cctgagacac cttaagaaca ccatggagac catagactgg
1981 aaggtctttg agagctggat gcaccattgg ctcctgtttg aaatgagcag gcactccttg
2041 gagcaaaagc ccactgacgc tccaccgaaa gagtcactgg aactggagga cccgtcttct
2101 gggctgggtg tgaccaagca ggatctgggc ccagtcccca tgtgaggatc cgcggccgca
2161 tagataactg atccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg
2221 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
2281 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
2341 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
2401 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
2461 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccccccc
2521 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
2581 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
2641 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
2701 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
2761 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
2821 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
2881 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
2941 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
3001 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
3061 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg ggaggcgcgc
3121 catgaagtgc cttttgtact tagccttttt attcattggg gtgaattgca agttcaccat
3181 agtttttcca cacaaccaaa aaggaaactg gaaaaatgtt ccttctaatt accattattg
3241 cccgtcaagc tcagatttaa attggcataa tgacttaata ggcacagcct tacaagtcaa
3301 aatgcccaag agtcacaagg ctattcaagc agacggttgg atgtgtcatg cttccaaatg
3361 ggtcactact tgtgatttcc gctggtatgg accgaagtat ataacacatt ccatccgatc
3421 cttcactcca tctgtagaac aatgcaagga aagcattgaa caaacgaaac aaggaacttg
3481 gctgaatcca ggcttccctc ctcaaagttg tggatatgca actgtgacgg atgccgaagc
3541 agtgattgtc caggtgactc ctcaccatgt gctggttgat gaatacacag gagaatgggt
3601 tgattcacag ttcatcaacg gaaaatgcag caattacata tgccccactg tccataactc
3661 tacaacctgg cattctgact ataaggtcaa agggctatgt gattctaacc tcatttccat
3721 ggacatcacc ttcttctcag aggacggaga gctatcatcc ctgggaaagg agggcacagg
3781 gttcagaagt aactactttg cttatgaaac tggaggcaag gcctgcaaaa tgcaatactg
3841 caagcattgg ggagtcagac tcccatcagg tgtctggttc gagatggctg ataaggatct
3901 ctttgctgca gccagattcc ctgaatgccc agaagggtca agtatctctg ctccatctca
3961 gacctcagtg gatgtaagtc taattcagga cgttgagagg atcttggatt attccctctg
4021 ccaagaaacc tggagcaaaa tcagagcggg tcttccaatc tctccagtgg atctcagcta
4081 tcttgctcct aaaaacccag gaaccggtcc tgctttcacc ataatcaatg gtaccctaaa
4141 atactttgag accagataca tcagagtcga tattgctgct ccaatcctct caagaatggt
4201 cggaatgatc agtggaacta ccacagaaag ggaactgtgg gatgactggg caccatatga
4261 agacgtggaa attggaccca atggagttct gaggaccagt tcaggatata agtttccttt
4321 atacatgatt ggacatggta tgttggactc cgatcttcat cttagctcaa aggctcaggt
4381 gttcgaacat cctcacattc aagacgctgc ttcgcaactt cctgatgatg agagtttatt
4441 ttttggtgat actgggctat ccaaaaatcc aatcgagctt gtagaaggtt ggttcagtag
4501 ttggaaaagc tctattgcct cttttttctt tatcataggg ttaatcattg gactattctt
4561 ggttctccga gttggtatcc atctttgcat taaattaaag cacaccaaga aaagacagat
4621 ttatacagac atagagatga accgacttgg aaagctgaat tccatggacg agaagaccac
4681 tggttggcga ggtggacacg ttgttgaagg actggctggg gaacttgaac aacttcgtgc
4741 acgactggag catcacccac aaggtcaacg tgaaccacct gcaggtatgg tgagcaaagg
4801 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
4861 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
4921 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
4981 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
5041 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
5101 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
5161 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
5221 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
5281 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
5341 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
5401 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
5461 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggccggc cttaaggcct
5521 cgagggccaa ttaattaact ctagataact gatcataatc agccatacca catttgtaga
5581 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
5641 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
5701 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
5761 actcatcaat gtatcttaac gcgtcgagtg cattctagtt gtggtttgtc caaactcatc
5821 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
5881 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
5941 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
6001 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
6061 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
6121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
6181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
6241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
6301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
6361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
6421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
6481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
6541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
6601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
6661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
6721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
6781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
6841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
6901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
6961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
7021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
7081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
7141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
7201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
7261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
7321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
7381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
7441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
7501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
7561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
7621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
7681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
7741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
7801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
7861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
7921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
7981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tga
//
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Str-Ii_VSVG-SBP-EGFP质粒载体应用举例
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