pYK479

LOCUS      pYK479              7352 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     145..187
                     /label=oYK244_tetO7_F
     misc_feature    206..225
                     /label=ZF1 DBM
     misc_feature    226..248
                     /label=spacer TET07
     misc_feature    249..268
                     /label=ZF1 DBM
     misc_feature    269..291
                     /label=spacer TET07
     misc_feature    292..311
                     /label=ZF1 DBM
     misc_feature    312..334
                     /label=spacer TET07
     misc_feature    335..354
                     /label=ZF1 DBM
     misc_feature    355..377
                     /label=spacer TET07
     misc_feature    378..397
                     /label=ZF1 DBM
     misc_feature    398..420
                     /label=spacer TET07
     misc_feature    421..440
                     /label=ZF1 DBM
     misc_feature    441..463
                     /label=spacer TET07
     misc_feature    464..483
                     /label=ZF1 DBM
     misc_feature    484..506
                     /label=spacer TET07
     misc_feature    507..526
                     /label=ZF1 DBM
     primer_bind     complement(532..572)
                     /label=oYK244_tetO7_R
     misc_feature    577..1164
                     /label=CMV
     primer_bind     1208..1227
                     /label=T7
     primer_bind     1226..1262
                     /label=oYK55-SEAP-F
     primer_bind     1226..1245
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     1245..1272
                     /label=oDJ273-1
     misc_feature    1246..2805
                     /label=SEAP
     primer_bind     complement(2784..2805)
                     /label=oDJ273-2
     primer_bind     complement(2786..2851)
                     /label=oYK98-SEAP-R
     primer_bind     complement(2786..2805)
                     /label=oYK55-SEAP-R
     primer_bind     complement(2787..2839)
                     /label=pYK62-SEAP-R
     primer_bind     complement(2789..2806)
                     /label=Oscl31-4
     primer_bind     complement(2789..2805)
                     /label=oSCL083
     polyA_signal    2797..2805
                     /label=SV40 late polyA
     misc_feature    2806..2831
                     /label=pA-SV40 (begin)
     terminator      2946..3173
                     /label=bGH PA
     misc_feature    3219..3647
                     /label=f1 origin
     misc_feature    3652..3995
                     /label=SV40 early promoter and origin
     misc_feature    3841..3918
                     /label=SV40 ORI
     CDS             4060..4851
                     /label=Kan/neoR
     misc_feature    5025..5155
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(5191..5211)
                     /label=M13-rev
     misc_binding    complement(5217..5239)
                     /label=LacO
     promoter        complement(5244..5273)
                     /label=lac
     misc_feature    complement(5538..6211)
                     /label=pUC origin
     CDS             complement(6359..7018)
                     /label=AmpR
     promoter        complement(7258..7286)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggtat cacgaggccc
      181 tttcgtcttc actcgagttt accaccgtcg aagtcgaagt cgaccaaagt gaaagtcgag
      241 tttaccaccg tcgaagtcga agtcgaccaa agtgaaagtc gagtttacca ccgtcgaagt
      301 cgaagtcgac caaagtgaaa gtcgagttta ccaccgtcga agtcgaagtc gaccaaagtg
      361 aaagtcgagt ttaccaccgt cgaagtcgaa gtcgaccaaa gtgaaagtcg agtttaccac
      421 cgtcgaagtc gaagtcgacc aaagtgaaag tcgagtttac caccgtcgaa gtcgaagtcg
      481 accaaagtga aagtcgagtt taccaccgtc gaagtcgaag tcgaccaaag tgaaagtcga
      541 gctcggtacc cgcgatgtac gggccagata taacgcgttg acattgatta ttgactagtt
      601 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      661 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      721 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      781 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      841 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      901 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      961 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1021 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1081 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1141 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     1201 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcatgct gctgctgctg
     1261 ctgctgctgg gcctgaggct acagctctcc ctgggcatca tcccagttga ggaggagaac
     1321 ccggacttct ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa gctgcagcct
     1381 gcacagacag ccgccaagaa cctcatcatc ttcctgggcg atgggatggg ggtgtctacg
     1441 gtgacagctg ccaggatcct aaaagggcag aagaaggaca aactggggcc tgagataccc
     1501 ctggccatgg accgcttccc atatgtggct ctgtccaaga catacaatgt agacaaacat
     1561 gtgccagaca gtggagccac agccacggcc tacctgtgcg gggtcaaggg caacttccag
     1621 accattggct tgagtgcagc cgcccgcttt aaccagtgca acacgacacg cggcaacgag
     1681 gtcatctccg tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc
     1741 acacgagtgc agcacgcctc gccagccggc acctacgccc acacggtgaa ccgcaactgg
     1801 tactcggacg ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga catcgctacg
     1861 cagctcatct ccaacatgga cattgacgtg atcctaggtg gaggccgaaa gtacatgttt
     1921 cgcatgggaa ccccagaccc tgagtaccca gatgactaca gccaaggtgg gaccaggctg
     1981 gacgggaaga atctggtgca ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg
     2041 aaccgcactg agctcatgca ggcttccctg gacccgtctg tgacccatct catgggtctc
     2101 tttgagcctg gagacatgaa atacgagatc caccgagact ccacactgga cccctccctg
     2161 atggagatga cagaggctgc cctgcgcctg ctgagcagga acccccgcgg cttcttcctc
     2221 ttcgtggagg gtggtcgcat cgaccatggt catcatgaaa gcagggctta ccgggcactg
     2281 actgagacga tcatgttcga cgacgccatt gagagggcgg gccagctcac cagcgaggag
     2341 gacacgctga gcctcgtcac tgccgaccac tcccacgtct tctccttcgg aggctacccc
     2401 ctgcgaggga gctccatctt cgggctggcc cctggcaagg cccgggacag gaaggcctac
     2461 acggtcctcc tatacggaaa cggtccaggc tatgtgctca aggacggcgc ccggccggat
     2521 gttaccgaga gcgagagcgg gagccccgag tatcggcagc agtcagcagt gcccctggac
     2581 gaagagaccc acgcaggcga ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg
     2641 gttcacggcg tgcaggagca gaccttcata gcgcacgtca tggccttcgc cgcctgcctg
     2701 gagccctaca ccgcctgcga cctggcgccc cccgccggca ccaccgacgc cgcgcacccg
     2761 ggttactcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     2821 agtttggaca aaagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg
     2881 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
     2941 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     3001 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     3061 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     3121 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     3181 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     3241 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3301 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3361 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3421 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3481 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3541 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3601 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     3661 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3721 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3781 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3841 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3901 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     3961 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     4021 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     4081 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4141 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4201 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     4261 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     4321 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     4381 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     4441 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     4501 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4561 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     4621 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4681 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4741 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4801 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     4861 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     4921 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     4981 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     5041 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5101 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     5161 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7321 gcacatttcc ccgaaaagtg ccacctgacg tc
//