| 质粒名称: | pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001737 |
| 产品用途: | 3' AAVLINK plasmid for RELN expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RELN |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-RELN-C-FLAG 7341 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7341)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7341
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..4054
/codon_start=1
/label=RELN
/translation="ALPGTCSWRFSMTSTVSPDL*ISFSLLMLKRLPLASAGGSQDMTA
WIRTTGPLTMSSSQALLTKGPLCWTPSAAPQYPSMSAPLQMPALSGGSPLTCLWKTKLQ
*MSTGYSMMIVQ*KDSVTPLMV*CSVAVMMDGRCMQ*PMT*LPLKAGLCNSRSQLDVRC
LKKLPRIKFMCSILLTSV*VGIIWSLSACLLTQNALEVFLSHLYSFQLKGGKGSPTHFL
KA*WEIR*GLGSIRSTQTCSGQSIISTWALDAWTTAGAMEIA*GNSASVIRDTQGQTAT
*PTL*RLS*RNALTVKKSNLTYGCP*KVEVLALSVEFLPRTLHSILGDPL*DKRLHKIW
IFEVQSSCNTGGASVVRTT*PLAIVPSAGRKACCWTTLPMEELPGLCSMRWITRNTFLL
DTTTYFFLKMPSPTQLDFAGGSLL*SAMELWSLGWSVLSGHWTTF*LVEQKSIPANWWT
LLMMKALPMKKTGVFTLML*GQQDFVAIHPFTSIGQIKRRTRLTMLSPPENSLYSQDT*
CSLKLWWVVKPLLVVTFIP*CWNTLRMQDRIPGSSYRPSAFLPLLTALAAPLSSSMKPP
STTLSTAQAGKESPSSCLTMSPLVQHSSAGSRREKKLRSKAGQLTTCTLERLAPSSAAG
TDTARPVPSASATRASKVMTALFSVTTFPVILKIILSPQESPRQTGRPFKVES*EVAVG
SWPPTPMETHCTLMAVRSGKQLPSLWISLEQAKSCLFCKLGACRRRTAATVT*VAPTLW
TRQCCCNTASTTGSPGMSSPSTSQRTSHKLRECLTMSPWRHG*KESYCAGGNHATMEQV
MINGLWTMWRSS**ALANKIT**IFHDNMGSDISTTEDEGHLGDT"
misc_feature 1556..1561
/label=SDS
CDS 4061..4126
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4130..4561
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4274..4279
/label=SDS
repeat_region 4604..4744
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4604..4733
/label=AAV2 ITR
rep_origin 4819..5274
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5556..5660
/gene="bla"
/label=AmpR promoter
CDS 5661..6521
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6692..7280
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggcattacct ggaacctgct
1501 catggagatt ttctatgacc agtacagtaa gcccggattt gtgaatatcc ttctccctcc
1561 tgatgctaaa gagattgcca ctcgcttccg ctggtggcag ccaagacatg acggcctgga
1621 tcagaacgac tgggccattg acaatgtcct catctcaggc tctgctgacc aaaggaccgt
1681 tatgctggac accttcagca gcgccccagt accccagcat gagcgctccc ctgcagatgc
1741 cggccctgtc gggaggatcg cctttgacat gtttatggaa gacaaaactt cagtgaatga
1801 gcactggcta ttccatgatg attgtacagt agaaagattc tgtgactccc ctgatggtgt
1861 gatgctctgt ggcagtcatg atggacggga ggtgtatgca gtgacccatg acctgactcc
1921 cactgaaggc tggattatgc aattcaagat ctcagttgga tgtaaggtgt ctgaaaaaat
1981 tgcccagaat caaattcatg tgcagtattc tactgacttc ggtgtgagtt ggaattatct
2041 ggtccctcag tgcttgcctg ctgacccaaa atgctctgga agtgtttctc agccatctgt
2101 attctttcca actaaagggt ggaaaaggat cacctaccca cttcctgaaa gcttagtggg
2161 aaatccggta aggtttaggt tctatcagaa gtactcagac atgcagtggg caatcgataa
2221 tttctacctg ggccctggat gcttggacaa ctgcaggggc catggagatt gcttaaggga
2281 acagtgcatc tgtgatccgg gatactcagg gccaaactgc tacttgaccc acactctgaa
2341 gactttcctg aaggaacgct ttgacagtga agaaatcaaa cctgacttat ggatgtcctt
2401 agaaggtgga agtacttgca ctgagtgtgg aattcttgcc gaggacactg cactctattt
2461 tgggggatcc actgtgagac aagcggttac acaagatttg gatcttcgag gtgcaaagtt
2521 cctgcaatac tgggggcgca tcggtagtga gaacaacatg acctcttgcc atcgtcccat
2581 ctgccggaag gaaggcgtgc tgttggacta ctctaccgat ggaggaatta cctggacttt
2641 gctccatgag atggattacc agaaatacat ttctgttaga cacgactaca tacttcttcc
2701 tgaagatgcc ctcaccaaca caactcgact tcgctggtgg cagccttttg tgatcagcaa
2761 tggaattgtg gtctctgggg tggagcgtgc tcagtgggca ctggacaaca ttttgattgg
2821 tggagcagaa atcaatccca gccaattggt ggacactttt gatgatgaag gcacttccca
2881 tgaagaaaac tggagttttt accctaatgc tgtaaggaca gcaggatttt gtggcaatcc
2941 atcctttcac ctctattggc caaataaaaa gaaggacaag actcacaatg ctctctcctc
3001 ccgagaactc attatacagc caggatacat gatgcagttt aaaattgtgg tgggttgtga
3061 agccacttct tgtggtgacc ttcattccgt aatgctggaa tacactaagg atgcaagatc
3121 ggattcctgg cagctcgtac agacccagtg ccttccttcc tcttctaaca gcattggctg
3181 ctcccctttc cagttccatg aagccaccat ctacaactct gtcaacagct caagctggaa
3241 aagaatcacc atccagctgc ctgaccatgt ctcctctagt gcaacacagt tccgctggat
3301 ccagaaggga gaagaaactg agaagcaaag ctgggcaatt gaccacgtgt acattggaga
3361 ggcttgcccc aagctctgca gcgggcacgg atactgcacg accggtgcca tctgcatctg
3421 cgacgagagc ttccaaggtg atgactgctc tgttttcagt cacgaccttc ccagttatat
3481 taaagataat tttgagtccg caagagtcac cgaggcaaac tgggagacca ttcaaggtgg
3541 agtcatagga agtggctgtg ggcagctggc cccctacgcc catggagact cactgtactt
3601 taatggctgt cagatcaggc aagcagctac caagcctctg gatctcactc gagcaagcaa
3661 aatcatgttt gttttgcaaa ttgggagcat gtcgcagacg gacagctgca acagtgacct
3721 gagtggcccc cacgctgtgg acaaggcagt gctgctgcaa tacagcgtca acaacgggat
3781 cacctggcat gtcatcgccc agcaccagcc aaaggacttc acacaagctc agagagtgtc
3841 ttacaatgtc cccctggagg cacggatgaa aggagtctta ctgcgctggt ggcaaccacg
3901 ccacaatgga acaggtcatg atcaatgggc tttggaccat gtggaggtcg tcctagtaag
3961 cactcgcaaa caaaattaca tgatgaattt ttcacgacaa catgggctca gacatttcta
4021 caacagaaga cgaaggtcac ttaggcgata cccatgtaca gactacaaag accatgacgg
4081 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac tcgagataat
4141 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
4201 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
4261 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc
4321 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
4381 gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg
4441 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
4501 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
4561 aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
4621 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
4681 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
4741 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
4801 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
4861 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
4921 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
4981 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
5041 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
5101 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
5161 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
5221 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
5281 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
5341 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
5401 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
5461 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
5521 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
5581 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
5641 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
5701 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
5761 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
5821 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
5881 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
5941 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
6001 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
6061 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
6121 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
6181 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
6241 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
6301 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
6361 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
6421 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
6481 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
6541 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
6601 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
6661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
6721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
6781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
6841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
6901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
6961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
7021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
7081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
7141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
7201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
7261 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
7321 gctggccttt tgctcacatg t
//
