psiCHECK-2-mBJ-SYT1_I368T-mScarlet3

LOCUS       psiCHECK-2-mBJ-SYT1_I368T-mScarlet3           6356 bp DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     688..715
                     /label=0709-EB3-fmStayG-F
     regulatory      688..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1395
                     /codon_start=1
                     /label=mBaoJin
                     /translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
                     LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
                     EAHLKGMDELYK"
     primer_bind     complement(1374..1399)
                     /label=0709-EB3-fmStayG-R
     CDS             1450..2718
                     /codon_start=1
                     /label=SYT1/I368T
                     /translation="MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKE
                     KFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINM
                     KDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGI
                     IQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGG
                     KTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL
                     RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYY
                     NESFSFEVPFEQIQKVQVVVTVLDYDKTGKNDAIGKVFVGYNSTGAELRHWSDMLANPR
                     RPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    2551..2553
                     /label=Mutation
     polyA_signal    2734..2782
                     /note="synthetic polyadenylation signal"
     promoter        2791..3542
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      3572..3581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3578..4264
                     /codon_start=1
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     3976..3995
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4311..4432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4565..4669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4670..5530
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5701..6289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggtct caaagggaga ggaggaaaac
      721 atggctagta caccatttaa atttcaactt aaaggaacca tcaatggcaa atcgtttacc
      781 gttgaaggcg aaggtgaagg gaactcacat gaaggttctc ataaaggaaa atatgtttgt
      841 acaagtggaa aactaccgat gtcatgggca gcacttggga caacctttgg ttatggaatg
      901 aaatattata ccaaatatcc tagtggactg aagaactggt ttcgtgaagt aatgcccgga
      961 ggctttacct acgatcgtca tattcaatat aaaggcgatg ggagtatcca tgcaaaacac
     1021 caacacttta tgaaaaatgg gacttatcac aacattgtag aatttaccgg tcaggatttt
     1081 aaagaaaata gtccagtctt aactggagat atgaatgtct cattaccgaa tgaagtccca
     1141 caaataccca gagatgatgg agtagaatgc ccagtgacct tgctttatcc tttattatcg
     1201 gataaatcaa aatacgtcga ggctcaccaa tatacaatct gcaagcctct tcataatcaa
     1261 ccagcacctg atgtcccata tcactggatt cgtaaacaat acacacaaag caaagatgat
     1321 gccgaggaac gcgatcatat ctgtcaatca gagactctcg aagcacactt aaagggcatg
     1381 gacgagctgt ataagtaatt ctaggcgatc gctcgagccc gggaattcgt ttaaacctag
     1441 agcggccgca tggtgagcga gagtcaccat gaggccctgg cagccccgcc tgtcaccact
     1501 gtcgcgactg ttctgccaag caatgccaca gagccagcca gtcctggaga aggaaaggaa
     1561 gatgcatttt ctaagctgaa ggagaagttt atgaatgagt tgcataaaat tccattgcca
     1621 ccgtgggcct taattgcaat agccatagtc gcagtccttt tagtcctgac ctgctgcttt
     1681 tgtatctgta agaaatgttt gttcaaaaag aaaaacaaga agaagggaaa ggaaaaagga
     1741 gggaagaatg ccattaacat gaaagatgta aaagacttag ggaagacgat gaaagatcag
     1801 gccctcaagg atgatgatgc tgaaactgga ttgacagatg gagaagaaaa agaagaaccc
     1861 aaagaagagg agaaactggg aaaacttcag tattcactgg attatgattt ccaaaataac
     1921 cagctgctgg tagggatcat tcaggctgcc gaactgcccg ccttggacat ggggggcaca
     1981 tctgatcctt acgtgaaagt gtttctgcta cctgataaga agaagaaatt tgagacaaaa
     2041 gtccaccgaa aaacccttaa tcctgtcttc aatgagcaat ttactttcaa ggtaccatac
     2101 tcggaattgg gtggcaaaac cctagtgatg gctgtatatg attttgatcg tttctctaag
     2161 catgacatca ttggagaatt taaagtccct atgaacacag tggattttgg ccatgtaact
     2221 gaggaatggc gtgacctgca aagtgctgag aaggaagagc aagagaaatt gggtgatatc
     2281 tgcttctccc ttcgctacgt acctactgct ggtaagctga ctgttgtcat tctggaggca
     2341 aagaacctga agaagatgga tgtgggtggc ttatccgatc cttatgtgaa gattcatctg
     2401 atgcagaatg gtaagaggct gaagaagaaa aagacaacaa ttaaaaagaa cacacttaac
     2461 ccctactaca atgagtcatt cagctttgaa gtaccttttg aacaaatcca gaaagtgcag
     2521 gtggtggtaa ctgttttgga ctatgacaag actggcaaga acgatgccat cggcaaagtc
     2581 tttgtgggct acaacagcac cggcgcggag ctgcgacact ggtcagacat gctggccaac
     2641 cccaggcgac ctattgccca gtggcacacc ctgcaggtag aggaggaagt tgatgccatg
     2701 ctggccgtca agaagtaagc ggccgctggc cgcaataaaa tatctttatt ttcattacat
     2761 ctgtgtgttg gttttttgtg tgaggatcta aatgagtctt cggacctcgc gggggccgct
     2821 taagcggtgg ttagggtttg tctgacgcgg ggggaggggg aaggaacgaa acactctcat
     2881 tcggaggcgg ctcggggttt ggtcttggtg gccacgggca cgcagaagag cgccgcgatc
     2941 ctcttaagca cccccccgcc ctccgtggag gcgggggttt ggtcggcggg tggtaactgg
     3001 cgggccgctg actcgggcgg gtcgcgcgcc ccagagtgtg accttttcgg tctgctcgca
     3061 gacccccggg cggcgccgcc gcggcggcga cgggctcgct gggtcctagg ctccatgggg
     3121 accgtatacg tggacaggct ctggagcatc cgcacgactg cggtgatatt accggagacc
     3181 ttctgcggga cgagccgggt cacgcggctg acgcggagcg tccgttgggc gacaaacacc
     3241 aggacggggc acaggtacac tatcttgtca cccggaggcg cgagggactg caggagcttc
     3301 agggagtggc gcagctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc
     3361 ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc
     3421 ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc caggtccact
     3481 tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt
     3541 aaaagcttgg cattccggta ctgttggtaa agccaccatg gatagcaccg aggcagtgat
     3601 caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga
     3661 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgagggt
     3721 gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg
     3781 ctccagggcc ttcacgaagc accccgccga catccccgac tactggaagc agtccttccc
     3841 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgtccgtggc
     3901 ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa
     3961 cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag catccaccga
     4021 gcggttgtac cccgaggacg tcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa
     4081 ggacggcggc cgctacctgg cggacttcaa gaccacctac agggccaaga agcccgtgca
     4141 gatgcccggc gccttcaaca tcgaccgcaa gttggacatc acatcccaca acgaggacta
     4201 caccgtggtg gaacagtacg aacgctccgt ggcccgccac tccaccggcg gctccggtgg
     4261 ctcctaataa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
     4321 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     4381 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     4441 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     4501 gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt ttcggggaaa
     4561 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4621 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4681 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4741 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4801 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4861 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4921 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4981 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5041 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5101 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5161 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5221 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5281 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5341 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5401 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5461 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5521 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5581 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5641 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5821 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgac
//