pAAVLINKv1-pCALM1-Cre-3'-RFX7-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-RFX7-FLAG                7017 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3730
                     /codon_start=1
                     /gene="RFX7"
                     /gene_synonym="MRD71; RFXDC2"
                     /product="DNA-binding protein RFX7 isoform 1"
                     /label=RFX7
                     /note="isoform 1 is encoded by transcript variant 
                     1;regulatory factor X domain containing 2; DNA-binding 
                     protein RFX7; regulatory factor X domain-containing protein
                     2; regulatory factor X, 7"
                     /db_xref="CCDS:CCDS92005.1"
                     /db_xref="GeneID:64864"
                     /db_xref="HGNC:HGNC:25777"
                     /db_xref="MIM:612660"
                     /protein_id="NP_073752.6"
                     /translation="AQIPSKVSVNVSSHIGANQPLNSSALVISDSALEQQTTPSSSPDI
                     KVKLEGSVFLLDSDSKSVGSFNPNGWQQITKDSEFISASCEQQQDISVMTIPEHSDIND
                     LEKSVWELEGMPQDTYSQQLHSQIQESSLNQIQAHSSDQLPLQSELKEFEPSVSQTNES
                     YFPFDDELTQDSIVEELVLMEQQMSMNNSHSYGNCLGMTLQSQSVTPGAPMSSHTSSTH
                     FYHPIHSNGTPIHTPTPTPTPTPTPTPTPTPTSEMIAGSQSLSRESPCSRLAQTTPVDS
                     ALGSSRHTPIGTPHSNCSSSVPPSPVECRNPFAFTPISSSMAYHDASIVSSSPVKPMQR
                     PMATHPDKTKLEWMNNGYSGVGNSSVSGHGILPSYQELVEDRFRKPHAFAVPGQSYQSQ
                     SRHHDTHFGRLTPVSPVQHQGATVNNTNKQEGFAVPAPLDNKGTNSSASSNFRCRSVSP
                     AVHRQRNLSGSTLYPVSNIPRSNVTPFGSPVTPEVHVFTNVHTDACANNIAQRSQSVPL
                     TVMMQTAFPNALQKQANSKKITNVLLSKLDSDNDDAVRGLGMNNLPSNYTARMNLTQIL
                     EPSTVFPSANPQNMIDSSTSVYEFQTPSYLTKSNSTGQINFSPGDNQAQSEIGEQQLDF
                     NSTVKDLLSGDSLQTNQQLVGQGASDLTNTASDFSSDIRLSSELSGSINDLNTLDPNLL
                     FDPGRQQGQDDEATLEELKNDPLFQQICSESMNSMTSSGFEWIESKDHPTVEMLG"
     misc_feature    2769..2774
                     /label=SDS
     CDS             3737..3802
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3806..4237
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3950..3955
                     /label=SDS
     repeat_region   4280..4420
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4280..4409
                     /label=AAV2 ITR
     repeat_region   4281..4421
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4495..4950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5232..5336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5337..6197
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6368..6956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgctcaga ttcctagcaa
     1501 ggtatcagta aatgtcagtt cacacatagg agcaaatcaa cccttgaatt cctctgccct
     1561 tgttatcagt gattcagctt tggaacagca aacaacccca tcatcatctc cagatataaa
     1621 agtaaaactt gaaggaagtg tctttctctt ggacagtgat tcaaagtcag ttggcagctt
     1681 taatccaaat ggatggcaac aaatcactaa agattctgag tttatatctg ccagttgtga
     1741 acaacagcaa gatatcagtg ttatgacaat tcctgagcac tctgatatca atgacttaga
     1801 gaaatctgtt tgggaattag aaggaatgcc acaggacaca tatagccagc agctacatag
     1861 ccagatacag gaatcttctt taaatcaaat acaagcacat tcttcagatc agttacctct
     1921 gcaatctgaa ctgaaggagt ttgagccttc tgtttcccag acaaatgaaa gctactttcc
     1981 ttttgatgat gaacttacac aagatagtat tgtggaagag ctggtgctta tggagcagca
     2041 aatgtcaatg aacaattctc attcttacgg caactgtttg ggaatgaccc ttcagagtca
     2101 gtcagtaact ccaggagctc caatgtcatc tcacacttcc agcacccact tctatcatcc
     2161 aatccacagc aatggcactc caatccacac acccacaccc acacccacac ccactcctac
     2221 tccaacccca accccaaccc cgacatctga aatgattgct ggatctcaga gtctgtcacg
     2281 ggagagccct tgctccaggc tagcccagac tacacctgtg gatagtgctt taggaagtag
     2341 ccgacataca cccattggta ctccacattc taactgcagc agtagtgtcc cccccagccc
     2401 tgttgaatgc aggaatccgt ttgcattcac tccaataagc tccagtatgg catatcatga
     2461 cgccagcatt gtctcaagta gtcctgtgaa accgatgcaa agacccatgg ccacacaccc
     2521 tgacaaaacc aagcttgaat ggatgaataa tgggtatagt ggggttggta attcatcagt
     2581 ttctggccat ggtattctcc caagctatca ggaactagtg gaagaccgtt tcaggaaacc
     2641 tcatgctttt gctgtgcctg gacagtctta tcagtctcaa tccagacatc atgacactca
     2701 ttttggtcgt ttgactcctg tctctcctgt gcagcatcaa ggtgccactg taaataacac
     2761 caacaaacag gagggttttg cagtccctgc ccctcttgat aataaaggaa ctaattcatc
     2821 tgccagcagc aacttcagat gccggagtgt gagccctgct gttcatcgcc aacgtaatct
     2881 tagtggaagc accctctatc cagtatctaa tatcccacga tctaatgtga ccccctttgg
     2941 aagtccagtt accccagaag ttcatgtttt cacaaatgtt cacacagacg catgtgccaa
     3001 caacatagct caaagaagcc aatcagttcc attgacagtc atgatgcaga cagccttccc
     3061 aaacgctctt cagaagcaag caaacagtaa aaaaataacc aatgttttgt tgagtaaact
     3121 tgattccgac aatgatgatg cagtgagagg tttgggaatg aacaacctgc cctctaatta
     3181 tacagcccgg atgaatctca ctcagatttt ggaaccttcc actgtttttc ctagtgccaa
     3241 cccacaaaat atgatcgatt ccagcacttc tgtttatgag ttccaaacac catcttacct
     3301 caccaaaagt aatagcaccg gtcagatcaa tttttctcct ggagataatc aagcacaatc
     3361 agaaattgga gagcaacaat tagatttcaa tagcactgtt aaagacctgt tgagtggaga
     3421 cagcttgcaa accaaccagc agctggtagg tcagggagca tctgatctca ctaatactgc
     3481 atctgatttc tctagcgata tcaggttgtc ttctgagctc tcaggcagca tcaatgattt
     3541 gaacacttta gacccaaatc tactgtttga tccaggtcgt cagcagggac aagatgatga
     3601 agctacactg gaagaattaa agaatgaccc attatttcaa caaatttgca gtgaatccat
     3661 gaattctatg acttcatcag gttttgaatg gatagaaagc aaggaccatc ctactgttga
     3721 aatgttgggt tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga
     3781 ctacaaggat gacgatgaca agtaactcga gataatcaac ctctggatta caaaatttgt
     3841 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3901 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3961 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4021 acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat
     4081 gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt
     4141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4201 gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac
     4261 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4321 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4381 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     4441 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     4501 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4561 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4621 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4681 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4741 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4801 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     4861 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4921 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4981 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5041 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5101 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5161 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//