RaMssiumER

LOCUS       RaMssiumER                9338 bp DNA     circular SYN 17-NOV-2024
DEFINITION  3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    pLJM1-RaMssumER
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
FEATURES             Location/Qualifiers
     source          1..9338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     444..464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             588..605
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1812..1829
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1842..2513
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="DNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGVV
                     EVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISHA
                     LKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHSG
                     SQ"
     misc_feature    2580..2697
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2746..3256
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3175..3193
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3278..3877
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3278..3297)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3774..3794
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4005..4238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4310..4431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4347..4366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4401..4420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4471..4606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4533..4552
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4556..4567)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4626..4645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4627..4645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4655..4672)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4655..4671)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4664..4686)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4813..5268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4900..4919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5110..5131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5294..5398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5399..6259
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5617..5636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6430..7018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6919..6938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7172..7189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7306..7327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7342..7372
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7380..7396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7385..7407
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7404..7420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7404..7420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7439..7459
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7441..7459
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7487..7713
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7714..7894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7941..8066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8559..8792
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8977..9021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(9319..9338,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag catgctgcta tccgtgccgt
      541 tgctgctcgg cctcctcggc ctggccgtcg ccgaccggtc ggggagccac catcaccatc
      601 accatggcag atctatggtc tcaaagggag aggaggaaaa catggctagt ctgcctgcta
      661 cccacgaact gcatattttt ggctccataa atggcatcga tttcgacatg gtaggccaag
      721 gcaccggtaa tccaaacgac ggctatgagg agctgaacct gaagtccacc atgggcgatc
      781 tgcagttcag tccctggatc ctggtgccgc acattgggta cgggtttcac cagtatcttc
      841 cctatccaga cgggatgtca ccatttcagg ctgcaatggt cgacgggtcc ggataccaag
      901 tgcaccgcac tatgcagttt gaggacggtg cctcactgac cgtgaattac agatacacct
      961 acgaaggatc acatatcaaa ggggaagctc aggtggaggg cactgggttc ccagctgacg
     1021 gccctgtgat gacaaattcc ctgacagccg aggcccacgg cctgttcaac ttcgtgaagg
     1081 acgccggcgc caagctgtgg gatgccgtga caggccagca cgacaaggac gaccaggcca
     1141 agaaggtgca ggaacacctg aacaagaccg gcatccccga cgccgacaaa gtgaacatcc
     1201 agatcgccga cggcaaggcc acagtgacag gcgacggact gagccaggaa gccaaagaaa
     1261 agatcctggt ggccgtgggc aacatcagcg gaatcgccag cgtggacgat caagtgaaaa
     1321 ccgccacccc tgccaccgcc agccagttct acacagtgaa gtccggcgac accctgagcg
     1381 ccatcagcaa acaggtgtac ggcaacgcca acctgtacaa caagattttc gaggccaaca
     1441 agcccatgct gaagtccccc gacaaaatct accccggcca ggtgctgaga atccccgagg
     1501 aaatgtactt cgctgactgg tgcgtatcca agaagacttg ccccaatgat aagactattg
     1561 tctccacatt taagtgggct tttatcaccg acaacggcaa acgctaccgg tctaccgctc
     1621 gcaccacata cacattcgcc aagccaatgg ccgcaaatta cctcaaaaat cagccgatgt
     1681 atgtctttcg gaaaacagag ctgaaacatt ctaagaccga actgaatttt aaagagtggc
     1741 aaaaggcctt tacggacgtg atgggcatgg acgagctgta taagggatcc gcgtctggtg
     1801 gtggtggtgg tctagttcca cgtggatctg cctcaggagc agacaacacc gaggacgtca
     1861 tcaaggagtt catgcagttc aaggtgcgca tggagggctc cgtgaacggc cactacttcg
     1921 agatcgaggg cgagggcgag ggcaagccct acgagggcac ccagaccgcc aagctgcagg
     1981 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
     2041 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacatgaag ctgtccttcc
     2101 ccgagggctt cacctgggag cgctccatga acttcgagga cggcggcgtg gtggaggtgc
     2161 agcaggactc ctccctgcag gacggcacct tcatctacaa ggtgaagttc aagggcgtga
     2221 acttccccgc cgacggcccc gtaatgcaga agaagactgc cggctgggag ccctccaccg
     2281 agaagctgta cccccaggac ggcgtgctga agggcgagat ctcccacgcc ctgaagctga
     2341 aggacggcgg ccactacacc tgcgacttca agaccgtgta caaggccaag aagcccgtgc
     2401 agctgcccgg caaccactac gtggactcca agctggacat caccaaccac aacgaggact
     2461 acaccgtggt ggagcagtac gagcacgccg aggcccgcca ctccggctcc cagggactca
     2521 gatcttacaa ggacgagctg taactcgacc tcgagacaaa tggcagtatt catccacaat
     2581 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2641 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2701 gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg
     2761 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     2821 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     2881 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     2941 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     3001 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     3061 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     3121 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     3181 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     3241 cgacctctct ccccaggggg atccaccgga gcttaccatg accgagtaca agcccacggt
     3301 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     3361 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     3421 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3481 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3541 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3601 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     3661 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     3721 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     3781 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3841 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     3901 cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa tgacttacaa
     3961 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4021 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4261 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4441 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4501 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4561 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     4621 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     4681 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4741 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4801 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5041 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5281 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5341 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5401 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5461 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5521 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5581 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5641 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5701 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5761 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5821 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5881 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5941 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6001 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6061 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6121 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6181 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6241 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6301 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6361 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6421 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6481 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6541 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6601 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6661 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6721 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6781 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6841 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6901 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6961 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7021 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7081 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7141 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7201 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7261 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7321 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7381 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7441 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     7501 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     7561 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     7621 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     7681 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     7741 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7801 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7861 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7921 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7981 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8041 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8101 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8161 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8221 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8281 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8341 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8401 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8461 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8521 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8581 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8641 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8701 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8761 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8821 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8881 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8941 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9001 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9061 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9121 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9181 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     9241 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     9301 gcggccgccc ccttcaccga gggcctattt cccatgat
//