| 质粒名称: | DT960 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 80412 |
| 存储实验室: | Thomas Cooper |
|
载体骨架基本信息 |
|
| 载体名称: | sp72 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2400bp |
| 完整质粒大小: | 8567bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | human DMPK exons 11-15 with 960 interrupted CTG repeats |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 6240bp |
|
文献引用方法 |
|
| 材料和方法部分: | DT960 was a gift from Thomas Cooper (Addgene plasmid # 80412 ; http://n2t.net/addgene:80412 ; RRID:Addgene_80412) |
| 参考文献部分: | Muscleblind proteins regulate alternative splicing. Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15. 10.1038/sj.emboj.7600300 PubMed 15257297 |
买家导航
DT960载体质粒基本信息
DT960质粒图谱载体图谱和DT960载体序列质粒序列多克隆位点信息
DT960质粒载体简介
DT960质粒序列载体序列
LOCUS DT960 8567 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses 960 interrupted CUG repeats in the context of human DMPK
genomic segment containing exons 11-15 with repeats inserted at site
in exon 15 that contains the repeats.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8567)
AUTHORS Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA
TITLE Muscleblind proteins regulate alternative splicing.
JOURNAL EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.
PUBMED 15257297
REFERENCE 2 (bases 1 to 8567)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J.";
date: "2004-08-4"; volume: "23(15)"; pages: "3103-12. Epub 2004 Jul
15"
COMMENT Sequence Label: DT960
FEATURES Location/Qualifiers
source 1..8567
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 21..40
/label=T7
/note="T7 promoter, forward primer"
promoter 21..39
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 139..442
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 443..646
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 597..617
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
promoter complement(6242..6260)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(6243..6260)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6341..6360)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6460..6482
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6520..6538)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6606..6710
/gene="bla"
/label=AmpR promoter
CDS join(6711..6779,6780..7571)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6929..6948)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7742..8330
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8484..8501
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ttaacctggc ttatcgaaat taatacgact cactataggg agaccggcag atctgatatc
61 atcgatgaat tcgagctcgg taccctaata gtaatcaatt acggggtcat tagttcatag
121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
661 tcagatccgc tagcgctacc ggactcagat caggctgagg ccgaggtgac gctgcgggag
721 ctccaggaag ccctggagga ggaggtgctc acccggcaga gcctgagccg ggagatggag
781 gccatccgca cggacaacca gaacttcgcc aggtcgggat cggggccggg gccggggccg
841 ggatgcgggc cggtggcaac ccttggcagc ccctctcgtc cggcccggac ggactcaccg
901 tccttacctc cccacagtca actacgcgag gcagaggctc ggaaccggga cctagaggca
961 cacgtccggc agttgcagga gcggatggag ttgctgcagg cagagggagc cacaggtgag
1021 tccctcatgt gtccccttcc ccggaggacc gggaggaggt gggccgtctg ctccgcgggg
1081 cgtgtataga cacctggagg agggaaggga cccacgctgg ggcacgccgc gccaccgccc
1141 tccttcgccc ctccacgcgc cctatgcctc tttcttctcc ttccagctgt cacgggggtc
1201 cccagtcccc gggccacgga tccaccttcc catgtaagac ccctctcttt cccctgcctc
1261 agacctgctg cccattctgc agatcccctc cctggctcct ggtctccccg tccagatata
1321 gggctcaccc tacgtctttg cgactttaga gggcagaagc cctttattca gccccagatc
1381 tccctccgtt caggcctcac cagattccct ccgggatctc cctagataac ctccccaacc
1441 tcgattccgc tcgctgtctc tcgccccacc gctgagggct gggctgggct ccgatcgggt
1501 cacctgtccc ttctctctcc agctagatgg ccccccggcc gtggctgtgg gccagtgccc
1561 gctggtgggg ccaggcccca tgcaccgccg ccacctgctg ctccctgcca gggtacgtcc
1621 ggctgcccac gcccccctcc gccgtcgcgc cccgcgctcc acccgccccg tgccacccgc
1681 ttagctgcgc atttgcgggg ctgggcccac ggcaggaggg cggatcttcg ggcagccaat
1741 caacacaggc cgctaggaag cagccaatga cgagttcgga cgggattcga ggcgtgcgag
1801 tggactaaca acagctgtag gctgttgggg cgggggcggg gcgcagggaa gagtgcgggc
1861 ccacctatgg gcgtaggcgg ggcgagtccc aggagccaat cagaggccca tgccgggtgt
1921 tgacctcgcc ctctccccgc aggtccctag gcctggccta tcggaggcgc tttccctgct
1981 cctgttcgcc gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt tggtggccca
2041 cgccggccaa ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc cctgaaccct
2101 agaactgtct tcgactccgg ggccccgttg gaagactgag tgcccggggc acggcacaga
2161 agccgcgccc accgcctgcc agttcacaac cgctccgagc gtgggtctcc gcccagctcc
2221 agtcctgtga tccgggcccg ccccctagcg gccggggagg gaggggccgg gtccgcggcc
2281 ggcgaacggg gctcgaaggg tccttgtagc cgggaatgtc gactgctgct gctgctgctg
2341 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
2401 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
2461 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
2521 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
2581 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
2641 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
2701 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
2761 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
2821 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
2881 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
2941 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
3001 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
3061 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
3121 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
3181 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
3241 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
3301 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
3361 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
3421 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
3481 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
3541 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
3601 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
3661 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
3721 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
3781 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
3841 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
3901 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
3961 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
4021 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
4081 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
4141 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
4201 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
4261 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
4321 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
4381 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
4441 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
4501 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
4561 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
4621 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
4681 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
4741 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
4801 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
4861 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
4921 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
4981 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
5041 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
5101 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
5161 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
5221 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
5281 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
5341 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
5401 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gacgggggat cacagaccat
5461 ttctttcttt cggccaggct gaggccctga cgtggatggg caaactgcag gcctgggaag
5521 gcagcaagcc gggccgtccg tgttccatcc tccacgcacc cccacctatc gttggttcgc
5581 aaagtgcaaa gctttcttgt gcatgacgcc ctgctctggg gagcgtctgg cgcgatctct
5641 gcctgcttac tcgggaaatt tgcttttgcc aaacccgctt tttcggggat cccgcgcccc
5701 cctcctcact tgcgctgctc tcggagcccc agccggctcc gcccgcttcg gcggtttgga
5761 tatttattga cctcgtcctc cgactcgctg acaggctaca ggacccccaa caaccccaat
5821 ccacgttttg gatgcactga gaccccgaca ttcctcggta tttattgtct gtccccacct
5881 aggaccccca cccccgaccc tcgcgaataa aaggccctcc atctgcccaa agctctggac
5941 tccacagtgt ccgcggtttg cgttgtgggc cggagctccg cagcgggcca atccggaggc
6001 gtgtggaggc ggccgaaggt ctgggaggag ctagcgggat gcgaagcggc cgaatcaggg
6061 ttgggggagg aaaagccacg gggcggggct ttggcgtccg gccaatagga gggcgagcgg
6121 gccacccgga ggcaccgccc ccgcccagct gtggcccagc tgtgccaccg agcgtcgaga
6181 agagggggct gggctggcag cgcgcgcggc catcctcctt ccactgcgcc tgcgctcgag
6241 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
6301 cgcgttctaa cgacaatatg tccatatggt gcactctcag tacaatctgc tctgatgccg
6361 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
6421 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
6481 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
6541 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
6601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
6661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
6721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
6781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
6841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
6901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
6961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
7021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
7081 ccataagcat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
7141 aggagctaac cgcttttttt cacaacatgg gggatcatgt aactcgcctt gatcgttggg
7201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
7261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
7321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
7381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
7441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggca
7501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
7561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
7621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
7681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
7741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
7801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
7861 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
7921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
7981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
8041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
8101 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
8161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
8221 agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
8281 ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa aacgccagca
8341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
8401 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
8461 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
8521 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagg
//
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