| 出品公司: | New England Biolabs |
|---|---|
| 载体名称: | pKLAC2 |
| 质粒类型: | 酵母表达质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 9107 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pKLAC2载体质粒基本信息
pKLAC2质粒图谱载体图谱和pKLAC2载体序列质粒序列多克隆位点信息
pKLAC2质粒载体简介
pKLAC2质粒序列载体序列
LOCUS pKLAC2 9107 bp ds-DNA circular SYN 29-NOV-2013
DEFINITION Integrative expression vector for the yeast Kluyveromyces lactis.
For an alternative MCS, use pKLAC1.
ACCESSION EU196354
VERSION .
KEYWORDS pKLAC2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9107)
AUTHORS New England Biolabs
TITLE Direct Submission
JOURNAL Exported Saturday, January 11, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT Linearize with SacII or BstXI to integrate at the LAC4 locus.
FEATURES Location/Qualifiers
source 1..9107
/organism="synthetic DNA construct"
/lab_host="Kluyveromyces lactis"
/mol_type="other DNA"
CDS 14..280
/codon_start=1
/product="N-terminal secretion signal from Kluyveromyces
lactis alpha-factor"
/note="alpha-factor secretion signal"
/note="Cleavage by the Kex2 protease occurs after the
dibasic KR sequence. The EA dipeptides are then removed by
dipeptidyl aminopeptidase A."
/translation="MKFSTILAASTALISVVMAAPVSTETDIDDLPISVPEEALIGFID
LTGDEVSLLPVNNGTHTGILFLNTTIAEAAFADKDDLEKREAEA"
misc_feature 257..359
/note="MCS"
/note="multiple cloning site"
terminator 371..989
/note="LAC4 terminator"
/note="transcription terminator for Kluyveromyces lactis
LAC4"
promoter 990..1692
/gene="S. cerevisiae ADH1"
/note="ADH1 promoter"
/note="promoter for alcohol dehydrogenase 1"
CDS 1713..3359
/codon_start=1
/gene="amdS"
/product="acetamidase from Aspergillus nidulans"
/note="amdS"
/note="confers ability to process acetamide for selection
in fungal cells"
/translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT
KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
promoter 3995..4651
/note="LAC4 promoter (5')"
/note="5' portion of a modified Kluyveromyces lactis LAC4
promoter"
rep_origin complement(5102..5690)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5861..6721)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6722..6826)
/gene="bla"
/note="AmpR promoter"
rep_origin 6853..7308
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7479..9007
/note="LAC4 promoter (3')"
/note="3' portion of a modified Kluyveromyces lactis LAC4
promoter"
ORIGIN
1 aagcttgaaa aaaatgaaat tctctactat attagccgca tctactgctt taatttccgt
61 tgttatggct gctccagttt ctaccgaaac tgacatcgac gatcttccaa tatcggttcc
121 agaagaagcc ttgattggat tcattgactt aaccggggat gaagtttcct tgttgcctgt
181 taataacgga acccacactg gtattctatt cttaaacacc accatcgctg aagctgcttt
241 cgctgacaag gatgatctcg agaaaagaga ggctgaagct agaagagctc atatgtccat
301 gggcggccgc gatatcgtcg acggatccga attccctgca ggtaattaaa taaaggcctt
361 gaatcgagaa tttatactta gataagtatg tacttacagg tatatttcta tgagatactg
421 atgtatacat gcatgataat atttaaacgg ttattagtgc cgattgtctt gtgcgataat
481 gacgttccta tcaaagcaat acacttacca cctattacat gggccaagaa aatattttcg
541 aacttgttta gaatattagc acagagtata tgatgttatc cgttagatta tgcatgattc
601 attcctacaa ctttttcgta gcataaggat taattacttg gatgccaata aaaaaaaaaa
661 acatcgagaa aatttcagca tgctcagaaa caattgcagt gtatcaaagt aaaaaaaaga
721 ttttcactac atgttccttt tgaagaaaga aaatcatgga acattagatt tacaaaaatt
781 taaccaccgc tgattaacga ttagaccgtt aagcgcacaa caggttatta gtacagagaa
841 agcattctgt ggtgttgccc cggactttct tttgcgacat aggtaaatcg aataccatca
901 tactatcttt tccaatgact ccctaaagaa agactcttct tcgatgttgt atacgttgga
961 gcatagggca agaattgtgg cttgagatca tccttttgtt gtttccgggt gtacaatatg
1021 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
1081 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
1141 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
1201 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
1261 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
1321 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
1381 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
1441 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
1501 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
1561 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
1621 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
1681 gcatacaatc aagcaattcc agatctgcca ccatgcctca atcctgggaa gaactggccg
1741 ctgataagcg cgcccgcctc gcaaaaacca tccctgatga atggaaagtc cagacgctgc
1801 ctgcggaaga cagcgttatt gatttcccaa agaaatcggg catcctttca gaggccgaac
1861 tgaagatcac agaggcttcc gctgcggatc ttgtgtccaa gctggcggcc ggagagttga
1921 cctcggtgga agttacgcta gcattctgta aacgggcagc aatcgcccag cagttaacaa
1981 actgcgccca cgagttcttc cctgacgccg ctctcgcgca ggcaagggaa ctcgatgaat
2041 actacgcaaa gcacaagaga cccgttggtc cacttcatgg cctccccatc tctctcaaag
2101 accagcttcg agtcaagggc tacgaaacat caatgggcta catctcatgg ctaaacaagt
2161 acgacgaagg ggactcggtt ctgacaacca tgctccgcaa agccggtgcc gtcttctacg
2221 tcaagacctc tgtcccgcag accctgatgg tctgcgagac agtcaacaac atcatcgggc
2281 gcaccgtcaa cccacgcaac aagaactggt cgtgcggcgg cagttctggt ggtgagggtg
2341 cgatcgttgg gattcgtggt ggcgtcatcg gtgtaggaac ggacatcggt ggctcgattc
2401 gagtgccggc cgcgttcaac ttcctgtacg gtctaaggcc gagtcatggg cggctgccgt
2461 atgcaaagat ggcgaacagc atggagggtc aggagacggt gcacagcgtt gtcgggccga
2521 ttacgcactc tgttgaggac ctccgcctct tcaccaaatc cgtcctcggt caggagcctt
2581 ggaaatacga ctccaaggtc atccccatgc cctggcgcca gtccgagtcg gacattattg
2641 cctccaagat caagaacggc gggctcaata tcggctacta caacttcgac ggcaatgtcc
2701 ttccacaccc tcctatcctg cgcggcgtgg aaactaccgt cgccgcactc gccaaagccg
2761 gtcacaccgt gaccccgtgg acgccataca agcacgattt cggccacgat ctcatctccc
2821 atatctacgc ggctgacggc agcgccgacg taatgcgcga catcagtgca tccggcgagc
2881 cggcgattcc aaatatcaaa gacctactga acccgaacat caaagctgtt aacatgaacg
2941 agctctggga cacgcatctc cagaagtgga attaccagat ggagtacctt gagaaatggc
3001 gggaggctga agaaaaggcc gggaaggaac tggacgccat catcgcgccg attacgccta
3061 ccgctgcggt acggcatgac cagttccggt actatgggta tgcctctgtg atcaacctgc
3121 tggatttcac gagcgtggtt gttccggtta cctttgcgga taagaacatc gataagaaga
3181 atgagagttt caaggcggtt agtgagcttg atgccctcgt gcaggaagag tatgatccgg
3241 aggcgtatca tggggcaccg gttgcagtgc aggttatcgg acggagactc agtgaagaga
3301 ggacgttggc gattgcagag gaagtgggga agttgctggg aaatgtggtg actccatagc
3361 ccgggggggg ctcgatcccc tcgcgagttg gttcagctgc tgcctgaggc tggacgacct
3421 cgcggagttc taccggcagt gcaaatccgt cggcatccag gaaaccagca gcggctatcc
3481 gcgcatccat gcccccgaac tgcaggagtg gggaggcacg atggccgctt tggtcgatct
3541 agattacgtg gaagaaaggt agtaaaagta gtagtataag tagtaaaaag aggtaaaaag
3601 agaaaaccgg ctacatacta gagaagcacg tacacaaaaa ctcataggca cttcatcata
3661 cgacagtttc ttgatgcatt ataatagtgt attagatatt ttcagaaata tgcatagaac
3721 ctcctcttgc ctttactttt tatacataga acattggcag atttacttac actactttgt
3781 ttctacgcca tttcttttgt tttcaacact tagacaagtt gttgagaacc ggactactaa
3841 aaagcaatgt tcccactgaa aatcatgtac ctgcagcata ataaccccct aattctgcat
3901 cgatccagta tgtttttttt tctctactca tttttacctg aagatagagc ttctaaaaca
3961 aaaaaaatca gtgattacat gcatattgtg tgttctagta accaaaggaa aggaacagat
4021 agataaaatt ccgagactgt caaattaggt ttttttcttt ttttttggcg ggagtcagtg
4081 ggccgaaata tgttcttggc ctagaactta atctggtttg atcatgccaa tacttgcctg
4141 agtgcccgac tttttgccca ccctcttgcc ttctgtcatc cttcaaaacc cacctgtttt
4201 ccagccgtat cttcgctcgc atctacacat actgtgccat atcttgtgtg tagccggacg
4261 tgactatgac caaaaacaaa caaggagaac tgttcgccga tttgtaacac tcctgcatcc
4321 atccaagtgg gtatgcgcta tgcaatgtta agctaggtca ggtcagacca ggtccaagga
4381 cagcaacttg actgtatgca acctttacca tctttgcaca gaacatactt gtagctagct
4441 agttacactt atggaccgaa aaggcacccc accatgtctg tccggcttta gagtacggcc
4501 gcagaccgct gatttgcctt gccaagcagt agtcacaatg catcgcatga gcacacgggc
4561 acgggcacgg gcacaggaac cattggcaaa aataccagat acactatacc gacgtatatc
4621 aagcccaagt ttaaaattcc taaatttccg cggctacttt tcaattccct atagtgagtc
4681 gtattaaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
4741 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
4801 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
4861 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
4921 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
4981 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
5041 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
5101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
5161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
5221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
5281 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
5341 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
5401 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
5461 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
5521 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
5581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
5641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
5701 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
5761 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
5821 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
5881 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
5941 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
6001 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
6061 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
6121 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
6181 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
6241 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
6301 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
6361 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
6421 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
6481 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
6541 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
6601 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
6661 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
6721 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
6781 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
6841 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
6901 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
6961 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
7021 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
7081 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
7141 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
7201 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
7261 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
7321 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
7381 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
7441 acgacgttgt aaaacgacgg ccagtgccaa gctcccgcgg ggatcgactc ataaaatagt
7501 aaccttctaa tgcgtatcta ttgactacca accattagtg tggttgcaga aggcggaatt
7561 ttcccttctt cgaatttagc ttgctttttc attttttatt ttccattttt cagtttttgt
7621 ttgtgtcgaa tttagccagt tgcttctcca agatgaaaaa aacccctgcg cagtttctgt
7681 gctgcaagat cctaatcgac ttttccaccc cccacaaaag taaatgttct tttgttacat
7741 tcgcgtgggt agctagctcc ccgaatcttc aaaggactta gggactgcac tacatcagag
7801 tgtgttcacc tggtttgctg cctggtttga aagaaaagag cagggaactc gcgggttccc
7861 ggcgaataat catgcgatag tcctttggcc ttccaagtcg catgtagagt agacaacaga
7921 cagggagggc aggaaggatc tttcactgag atcctgtatc ttgttgggta agtcggatga
7981 aaggggaatc gtatgagatt ggagaggatg cggaagaggt aacgcctttt gttaacttgt
8041 ttaattatta tggggcaggc gagaggggga ggaatgtatg tgtgtgaggc gggcgagacg
8101 gagccatcca ggccaggtag aaatagagaa agccgaatgt tagacaatat ggcagcgtag
8161 tagagtaggt aggtaggcaa gtactgctag caaagaggag aagggtaagc tcactcttcg
8221 cattccacac cgttagtgtg tcagtttgaa caaaaaaaca atcatcatac caattgatgg
8281 actgtggact ggcttttgga acggcttttc ggactgcgat tattcgtgag gaatcaaggt
8341 aggaatttgg tcatatttac ggacaacagt gggtgattcc catatcgagt aggaaaacga
8401 gatcatggta tcctcagata tgttgcggaa atctgttcac cgcaaagttc agggtgctct
8461 ggtgggtttc ggttggtctt tgctttgctt ctcccttgtc ttgcatgtta ataatagcct
8521 agcctgtgag ccgaaactta gggtaggctt agtgttggaa cgtacatatc tatcacgttg
8581 acttggttta accaggcgac ctggtagcca gccataccca cacacgtttt ttgtatcttc
8641 agtatagttg tgaaaagtgt agcggaaatt tgtggtccga gcaacagcgt ctttttctag
8701 tagtgcggtc ggttacttgg ttgacattgg tatttggact ttgttgctac accattcact
8761 acttgaagtc gagtgtgaag ggtatgattt ctagtggtga acacctttag ttacgtaatg
8821 ttttcattgc tgttttactt gagatttcga ttgagaaaaa ggtatttaat agctcgaatc
8881 aatgtgagaa cagagagaag atgttcttcc ctaactcgaa aggtatatga ggcttgtgtt
8941 tcttaggaga attattattc ttttgttatg ttgcgcttgt agttggaaaa ggtgaagaga
9001 caaaagctgg aattgtgagc ggataacaag ctcaacactt gaaatttagg aaagagcaga
9061 atttggcaaa aaaaataaaa aaaaaataaa cacacatact catcgag
//
