PE8c

LOCUS       PE8c        9358 bp    DNA     circular SYN 03-JUN-2026
DEFINITION  in vitro transcription plasmid for PE8c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Tao YA, Sakai HA, Jiang AY, Krasnow NA, Vaganov VS, Shim B, Barsdale
            Z, Pandey S, Ahmed N, Na M, Liao TW, Oye K, Cristian A, Zhang E, Xu 
            JA, Bulcaen M, Liu DR
  TITLE     AI-guided redesign of laboratory-evolved reverse transcriptases 
            enhances prime editing.
  JOURNAL   Nat Biotechnol. 2026 May 21. doi: 10.1038/s41587-026-03149-6.
  PUBMED    42168701
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2026 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2026 May 21. doi: 10.1038/s41587-026-03149-6."
COMMENT     Sequence Label: PE8c
FEATURES             Location/Qualifiers
     source          1..9358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    4..228
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(299..315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(299..315)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(312..334)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    323..339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(347..353,354..371,372..377))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    392..413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(530..547)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(701..1289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(781..800)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1460..2251,2252..2320))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2083..2102
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2321..2425)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2504..2523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2695..3074
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3075..3278
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3229..3249
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3414..3434
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3435..7535
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     
     CDS             7593..7613
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9198..9218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9228..9254
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
       61 ccctggaagg tgccactccc actgtccttt cctaataaaa tgagaaaatt gcatcgcatt
      121 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      181 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      241 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
      301 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      361 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
      421 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      481 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      541 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      601 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      661 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      721 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      781 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      841 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      901 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      961 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1021 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1081 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1141 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1201 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1261 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1321 acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1381 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1441 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1501 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1561 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1621 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1681 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1741 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1801 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1861 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1921 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1981 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2041 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2101 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2161 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2221 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2281 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2341 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2401 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     2461 gatcgggaga tcgatctccc gatcccctag ggtctactct cagtacaatc tgctctgatg
     2521 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     2581 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     2641 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     2701 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     2761 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2821 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2881 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2941 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3001 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3061 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     3121 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     3181 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3241 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatct
     3301 cgagctcggt acctaatacg acacactata aggaaataag agagaaaaga agagtaagaa
     3361 gaaatataag agccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga
     3421 agaagcggaa agtcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
     3481 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     3541 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
     3601 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     3661 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     3721 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     3781 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     3841 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     3901 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     3961 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     4021 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     4081 gcaagagcag aaagctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     4141 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     4201 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     4261 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     4321 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     4381 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     4441 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     4501 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     4561 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aagagagagg
     4621 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     4681 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     4741 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     4801 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     4861 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     4921 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     4981 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     5041 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     5101 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     5161 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     5221 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     5281 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     5341 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     5401 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     5461 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     5521 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     5581 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     5641 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     5701 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     5761 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     5821 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     5881 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     5941 atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     6001 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     6061 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     6121 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     6181 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     6241 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     6301 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     6361 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     6421 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     6481 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     6541 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     6601 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     6661 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     6721 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     6781 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     6841 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     6901 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     6961 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     7021 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     7081 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     7141 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     7201 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     7261 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     7321 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     7381 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     7441 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     7501 agacacggat cgacctgtct cagctgggag gtgactccgg cggaagctct ggtggcagca
     7561 agcggaccgc cgacggctct gaattcgaga gccctaagaa gaaaagaaag gtgagcggag
     7621 gctctagcgg cggaagcatc tcatcgtcga agcacacgct gagccagatg aataaggtct
     7681 ccaacatcgt gaaacctcct gagctcccgg agaagtacaa agaattcaaa gacattttta
     7741 agaaggaccc catcctcaag ctgccaaagc ccattccagg cttggaattt gaggtggagc
     7801 tgaccaaaga agacattacc ctgcccgaga gagactatcc actgacccct gagcagagag
     7861 cagccttaag agagtttatc gaacggggac tcaaaggagg catcattcga ccttctgact
     7921 ccaagaatgc ctgccctgtg atctttgtgc cccgcaagga tggcaccttg agaatggtgg
     7981 tcgactaccg gcctttaaac aaatacgtaa aacccaacgt gtaccctctg cctctgatcg
     8041 agcaactgct ggacaagctg acaggtggca ccatcttcac aaagctagac ctgaagagcg
     8101 cctactacca gatcagagtg aagcctggag acgagcacaa gctcgccttc cgtagccctc
     8161 tgggtgtgtt tgaatacttg gtcatgccat atgggatcaa gacagcccct gctcacttcc
     8221 agtatttcat taacactgtg ctgggggacc tgctggagac gcacgtggtc tgctacatgg
     8281 atgacatcct catcttcagc aaatcagaag aggagcatga caagcatgtg aaggaggttc
     8341 tgcagcggtt gaaggatgct ggcctgatcg taaacctgga aaagtgtgag ttccacaaaa
     8401 agtcagttaa gtttctgggc taccacatct ctgcagaggg cctgacacct tgccaggaga
     8461 atattgacaa ggtgctccag tggaagctgc ccaaaaacca gaaggaactg cggcagttcc
     8521 ttggccaggt gaattacctg aggaagttca tccccaagac tagccaactc acccacccac
     8581 tgaacaagct gctgaaaaaa gacgtgagat ggaaatggac agagaaacac acagaagcca
     8641 ttgaaaaaat aaaaagcttg ctgaagagtc cacctgtact ggcgcactat gacccaacca
     8701 agccaatccg gctggagact gacgtgtccg atgtggctgt gggagccgtg ctgtctcaac
     8761 tggatgagga tggaaagtgg agacctgtgg gctattacag tgccaagatg tctaaggccc
     8821 agctcaacta ctcagtcagc gataaagaga tgctagccat catcaagagc ctggaacact
     8881 ggaggcacta cctggaaaac cggaaggagc ccttcctgat cctgacagac cataagaacc
     8941 tgattgaaag aatcacctct cccaagaaac cagaaaacaa gagattggcc cgctggcagc
     9001 tcttcttaaa aaatttcaac ttcatcatcc tgtacaggcc tggcagcgaa aatgctctag
     9061 ctgatgcctt gagccgactc accgatgaag atgttcccga gccaccactc gatccaagca
     9121 acatcattcc cataataaat gagaaggaac cttctggcgg ctcaaaaaga accgccgacg
     9181 gcagcgaatt cgagtctccc aagaagaaga ggaaagtcgg ctctggccct gccgctaaga
     9241 gagtgaagct ggactaatta attaagctgc cttctgcggg gcttgccttc tggccatgcc
     9301 cttcttctct cccttgcacc tgtacctctt ggtctttgaa taaagcctga gtaggaag
//