| 质粒名称: | pJK1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 111722 |
| 存储实验室: | Ian Hickson |
|
载体骨架基本信息 |
|
| 载体名称: | pYES2 |
| 载体出品公司: | ThermoFisher |
| 完整质粒大小: | 10092bp |
| 载体类型: | Yeast Expression |
| 筛选标记: | URA3 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | BLM、BS、BS |
| 物种来源: | H. sapiens (human) |
| 基因突变: | C-terminal hexahistidine tag was added, a yeast Kozak consensus sequence was included upstream of the coding sequence, and the first five codons of S. cerevisiae TOP2 gene replaced the initiation codon of the BLM cDNA. |
| 启动子: | GAL1 |
| 标签/融合蛋白: | hexahistidine (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | known (unknown if destroyed) |
| 3’克隆位点: | known (unknown if destroyed) |
| 5’测序引物 | unknown |
|
文献引用方法 |
|
| 材料和方法部分: | pJK1 was a gift from Ian Hickson (Addgene plasmid # 111722 ; http://n2t.net/addgene:111722 ; RRID:Addgene_111722) |
| 参考文献部分: | The Bloom's syndrome gene product is a 3'-5' DNA helicase. Karow JK, Chakraverty RK, Hickson ID. J Biol Chem. 1997 Dec 5;272(49):30611-4. PubMed 9388193 |
买家导航
pJK1载体质粒基本信息
pJK1质粒图谱载体图谱和pJK1载体序列质粒序列多克隆位点信息
pJK1质粒载体简介
pJK1质粒序列载体序列
LOCUS pJK1 10092 bp DNA circular SYN 14-APR-2025
DEFINITION to express human BLM protein in yeast cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10092)
AUTHORS Karow JK, Chakraverty RK, Hickson ID
TITLE The Bloom's syndrome gene product is a 3'-5' DNA helicase.
JOURNAL J Biol Chem. 1997 Dec 5;272(49):30611-4.
PUBMED 9388193
REFERENCE 2 (bases 1 to 10092)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
Chem."; date: "1997-12-5"; volume: "272(49)"; pages: "30611-4"
COMMENT Sequence Label: pJK1
FEATURES Location/Qualifiers
source 1..10092
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..442
/gene="S. cerevisiae GAL1"
/label=GAL1 promoter
/note="inducible promoter, regulated by Gal4"
protein_bind 1..118
/label=UAS
/bound_moiety="Gal4"
/note="upstream activating sequence mediating
Gal4-dependent induction"
primer_bind 413..436
/label=GAL1
/note="S. cerevisiae GAL1 promoter, forward primer"
primer_bind 474..493
/label=T7
/note="T7 promoter, forward primer"
promoter 474..492
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4793..4810
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 4840..5087
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(4856..4874)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
primer_bind complement(5164..5181)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5335..5923)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5415..5434)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6094..6885,6886..6954))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6717..6736
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS complement(7050..7853)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(7854..8069)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
rep_origin 8670..9547
/label=2u ori
/note="yeast 2u plasmid origin of replication"
rep_origin complement(9558..10071)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9753..9774)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9965..9984
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
61 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
181 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
301 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
361 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
421 tctatacttt aacgtcaagg agaaaaaacc ccggatcgga ctactagcag ctgtaatacg
481 actcactata gggaatatta agcttggtac cgagctcgga tccctaacca tgtcaactga
541 accggctgct gttcctcaaa ataatctaca ggagcaacta gaacgtcact cagccagaac
601 acttaataat aaattaagtc tttcaaaacc aaaattttca ggtttcactt ttaaaaagaa
661 aacatcttca gataacaatg tatctgtaac taatgtgtca gtagcaaaaa cacctgtatt
721 aagaaataaa gatgttaatg ttaccgaaga cttttccttc agtgaacctc tacccaacac
781 cacgaatcag caaagggtca aggacttctt taaaaatgct ccagcaggac aggaaacaca
841 gagaggtgga tcaaaatcat tattgccaga tttcttgcag actccgaagg aagttgtatg
901 cactacccaa aacacaccaa ctgtaaagaa atcccgggat actgctctca agaaattaga
961 atttagttct tcaccagatt ctttaagtac catcaatgat tgggatgata tggatgactt
1021 tgatacttct gagacttcaa aatcatttgt tacaccaccc caaagtcact ttgtaagagt
1081 aagcactgct cagaaatcaa aaaagggtaa gagaaacttt tttaaagcac agctttatac
1141 aacaaacaca gtaaagactg acttgcctcc accctcctct gaaagcgagc aaatagattt
1201 gactgaggaa cagaaggatg actcagaatg gttaagcagc gatgtgattt gcatcgatga
1261 tggccccatt gctgaagtgc atataaatga agatgctcag gaaagtgact ctctgaaaac
1321 tcatttggaa gatgaaagag ataatagcga aaagaagaag aatttggaag aagctgaatt
1381 acattcaact gagaaagttc catgtattga atttgatgat gatgattatg atacggattt
1441 tgttccacct tctccagaag aaattatttc tgcttcttct tcctcttcaa aatgccttag
1501 tacgttaaag gaccttgaca cctctgacag aaaagaggat gttcttagca catcaaaaga
1561 tcttttgtca aaacctgaga aaatgagtat gcaggagctg aatccagaaa ccagcacaga
1621 ctgtgacgct agacagataa gtttacagca gcagcttatt catgtgatgg agcacatctg
1681 taaattaatt gatactattc ctgatgataa actgaaactt ttggattgtg ggaacgaact
1741 gcttcagcag cggaacataa gaaggaaact tctaacggaa gtagatttta ataaaagtga
1801 tgccagtctt cttggctcat tgtggagata caggcctgat tcacttgatg gccctatgga
1861 gggtgattcc tgccctacag ggaattctat gaaggagtta aatttttcac accttccctc
1921 aaattctgtt tctcctgggg actgtttact gactaccacc ctaggaaaga caggattctc
1981 tgccaccagg aagaatcttt ttgaaaggcc tttattcaat acccatttac agaagtcctt
2041 tgtaagtagc aactgggctg aaacaccaag actaggaaaa aaaaatgaaa gctcttattt
2101 cccaggaaat gttctcacaa gcactgctgt gaaagatcag aataaacata ctgcttcaat
2161 aaatgactta gaaagagaaa cccaaccttc ctatgatatt gataattttg acatagatga
2221 ctttgatgat gatgatgact gggaagacat aatgcataat ttagcagcca gcaaatcttc
2281 cacagctgcc tatcaaccca tcaaggaagg tcggccaatt aaatcagtat cagaaagact
2341 ttcctcagcc aagacagact gtcttccagt gtcatctact gctcaaaata taaacttctc
2401 agagtcaatt cagaattata ctgacaagtc agcacaaaat ttagcatcca gaaatctgaa
2461 acatgagcgt ttccaaagtc ttagttttcc tcatacaaag gaaatgatga agatttttca
2521 taaaaaattt ggcctgcata attttagaac taatcagcta gaggcgatca atgctgcact
2581 gcttggtgaa gactgtttta tcctgatgcc gactggaggt ggtaagagtt tgtgttacca
2641 gctccctgcc tgtgtttctc ctggggtcac tgttgtcatt tctcccttga gatcacttat
2701 cgtagatcaa gtccaaaagc tgacttcctt ggatattcca gctacatatc tgacaggtga
2761 taagactgac tcagaagcta caaatattta cctccagtta tcaaaaaaag acccaatcat
2821 aaaactccta tatgtcactc cagaaaagat ctgtgcaagt aacagactca tttctactct
2881 ggagaatctc tatgagagga agctcttggc acgttttgtt attgatgaag cacattgtgt
2941 cagtcagtgg ggacatgatt ttcgtcaaga ttacaaaaga atgaatatgc ttcgccagaa
3001 gtttccttct gttccggtga tggctcttac ggccacagct aatcccaggg tacagaagga
3061 catcctgact cagctgaaga ttctcagacc tcaggtgttt agcatgagct ttaacagaca
3121 taatctgaaa tactatgtat taccgaaaaa gcctaaaaag gtggcatttg attgcctaga
3181 atggatcaga aagcaccacc catatgattc agggataatt tactgcctct ccaggcgaga
3241 atgtgacacc atggctgaca cgttacagag agatgggctc gctgctcttg cttaccatgc
3301 tggcctcagt gattctgcca gagatgaagt gcagcagaag tggattaatc aggatggctg
3361 tcaggttatc tgtgctacaa ttgcatttgg aatggggatt gacaaaccgg acgtgcgatt
3421 tgtgattcat gcatctctcc ctaaatctgt ggagggttac taccaagaat ctggcagagc
3481 tggaagagat ggggaaatat ctcactgcct gcttttctat acctatcatg atgtgaccag
3541 actgaaaaga cttataatga tggaaaaaga tggaaaccat catacaagag aaactcactt
3601 caataatttg tatagcatgg tacattactg tgaaaatata acagaatgca ggagaataca
3661 gcttttggcc tactttggtg aaaatggatt taatcctgat ttttgtaaga aacacccaga
3721 tgtttcttgt gataattgct gtaaaacaaa ggattataaa acaagagatg tgactgacga
3781 tgtgaaaagt attgtaagat ttgttcaaga acatagttca tcacaaggaa tgagaaatat
3841 aaaacatgta ggtccttctg gaagatttac tatgaatatg ctggtcgaca ttttcttggg
3901 gagtaagagt gcaaaaatcc agtcaggtat atttggaaaa ggatctgctt attcacgaca
3961 caatgccgaa agacttttta aaaagctgat acttgacaag attttggatg aagacttata
4021 tatcaatgcc aatgaccagg cgatcgctta tgtgatgctc ggaaataaag cacaaactgt
4081 actaaatggc aatttaaagg tagactttat ggaaacagaa aattccagca gtgtgaaaaa
4141 acaaaaagcg ttagtagcaa aagtgtctca gagggaagag atggttaaaa aatgtcttgg
4201 agaacttaca gaagtctgca aatctctggg gaaagttttt ggtgtccatt acttcaatat
4261 ttttaatacc gtcactctca agaagcttgc agaatcttta tcttctgatc ctgaggtttt
4321 gcttcaaatt gatggtgtta ctgaagacaa actggaaaaa tatggtgcgg aagtgatttc
4381 agtattacag aaatactctg aatggacatc gccagctgaa gacagttccc cagggataag
4441 cctgtccagc agcagaggcc ccggaagaag tgccgctgag gagcttgacg aggaaatacc
4501 cgtatcttcc cactactttg caagtaaaac cagaaatgaa aggaagagga aaaagatgcc
4561 agcctcccaa aggtctaaga ggagaaaaac tgcttccagt ggttccaagg caaagggggg
4621 gtctgccaca tgtagaaaga tatcttccaa aacgaaatcc tccagcatca ttggatccag
4681 ttcagcctca catacttctc aagcgacatc aggagccaat agcaaattgg ggattatggc
4741 tccaccgaag cctataaata gaccgtttct taagccttca tatgcattct cacaccacca
4801 ccaccaccac tgactcgagc atgcatctag agggccgcat catgtaatta gttatgtcac
4861 gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac
4921 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta
4981 tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga
5041 aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag ctgcggccct
5101 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
5161 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
5221 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
5281 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
5341 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
5401 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
5461 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
5521 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
5581 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
5641 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
5701 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
5761 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
5821 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
5881 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
5941 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
6001 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
6061 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
6121 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
6181 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
6241 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
6301 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
6361 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
6421 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
6481 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
6541 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
6601 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
6661 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
6721 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
6781 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
6841 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
6901 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
6961 cctttttcaa tgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt
7021 atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat
7081 gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca
7141 aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt
7201 ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc
7261 aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca
7321 aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg
7381 gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct
7441 tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta
7501 atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta
7561 ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc
7621 tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt
7681 agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga
7741 acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca
7801 gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat
7861 cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat
7921 gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
7981 tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa
8041 aaagaataaa aaaaaaatga tgaattgaaa agctagctta tcgatgataa gctgtcaaac
8101 atgagaatta attccacgga ctatagacta tacctagtat actccgtcta ctgtacgata
8161 cacttccgct caggtccttg tcctttaacg aggccttacc actcttttgt tactctattg
8221 atccagctca gcaaaggcag tgtgatctaa gattctatct tcgcgatgta gtaaaactag
8281 ctagaccgag aaagagacta gaaatgcaaa aggcacttct acaatggctg ccatcattat
8341 tatccgatgt gacgctgcag cttctcaatg atattcgaat acgctttgag gagatacagc
8401 ctaatatccg acaaactgtt ttacagattt acgatcgtac ttgttaccca tcattgaatt
8461 ttgaacatcc gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat
8521 aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta
8581 ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc
8641 atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac
8701 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
8761 aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt
8821 aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
8881 tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt
8941 tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac
9001 tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt
9061 ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg
9121 actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa
9181 aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt
9241 gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct
9301 ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa
9361 tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt
9421 agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg
9481 atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat
9541 tcgcaatggg aagctccacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
9601 tataagcaaa tatttaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
9661 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
9721 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
9781 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
9841 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
9901 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
9961 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
10021 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatcga
10081 tccactagtc ca
//
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