| 质粒名称: | pWA23 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000158 |
| 产品用途: | Expresses the hemoxygenase in bacteria |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pWA21 |
| 表达系统: | 细菌表达 |
| 启动子: | rhamnose |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | Hemoxygenase |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
GAF-CaMP3-sfGFP, An Enhanced Version of the Near-Infrared Genetically Encoded Positive Phytochrome-Based Calcium Indicator for the Visualization of Neuronal Activity
Subach O.M., Subach F.V.
Int. J. Mol. Sci. 2020, Vol. 21, Page 6883 21, 6883 (2020)
|
买家导航
pWA23载体质粒基本信息
pWA23质粒图谱载体图谱和pWA23载体序列质粒序列多克隆位点信息
pWA23质粒载体简介
pWA23质粒序列载体序列
LOCUS pWA23 5562 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5562)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5562
/mol_type="other DNA"
/organism="synthetic DNA construct"
gene 20..703
/label=hmuO
terminator 912..998
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1047..1094
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(1324..2139)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
promoter complement(2140..2231)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2249..2884)
/direction=LEFT
/label=ColA ori
/note="Plasmids containing the ColA origin of replication
can be propagated in E. coli cells that contain additional
plasmids with compatible origins."
misc_feature 3300..3442
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3544..3735)
/codon_start=1
/gene="rop"
/product="Rop protein"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
promoter 5377..5395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 5407..5525
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
primer_bind 5439..5459
/label=rha-F
ORIGIN
1 ttaagaagga gatatacata tgttggaccg agttgattcc aatcacgtcg aaacggccgg
61 gcgcaccacg cgtccggccg gtcttttgga agccatgcgc gagcggacca agacgctgca
121 cgtgacggcg gagcggaccg gcgtcgtcgc cgagctgttg cgcggccgcg gcacggtgcg
181 cgcctatgcg ctgctgctgc gcaatctgct gccggtctat gaggcgctcg aagcggagct
241 cgttcgtcat caggcgtcac cggtggtcgg actgacggtg cggccggagc tgcatcggtg
301 tccagcgatc aaggccgatc tcgccgcgct cgacgcgagc gatctgccgt tgctgcccga
361 ggccatcgcc tatgttcgcg ccatccagga ggcgggatcc ggctccggtc atccgctgct
421 ggcgcacgcc tatacacgct atctcggtga tctcagcggc ggccagatca tcaagaagat
481 cctcgcccgc tcgctcgaac tgcagcccga ggcgctgtcg ttctacgagt ttccggcgat
541 caccgacatt ccgcgcttca agaccgagta ccgcgaggcg ctggagcagg ccggctcggc
601 gatgacagag catgactccg tcgtcgagga agcggccacg gctttccagc tgaacatcac
661 gctgtcgcag gcgctgcagt cggccgcggg gctcggccgc tgaaagcttg gctgttttgg
721 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
781 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
841 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
901 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
961 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgacct
1021 aggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
1081 tgaggggttt tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg
1141 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg
1201 aaccgacgac aagctgacga ccgggtctcc gcaagtggca cttttcgggg aaatgtgcgc
1261 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
1321 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
1381 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
1441 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
1501 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
1561 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
1621 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
1681 ttgcgcctga gcgagacgaa atacgcggtc gctgttaaaa ggacaattac aaacaggaat
1741 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
1801 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
1861 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
1921 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
1981 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
2041 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
2101 cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta
2161 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
2221 aaataaacaa ataggcatgc tagcgcagaa acgtcctaga agatgccagg aggatactta
2281 gcagagagac aataaggccg gagcgaagcc gtttttccat aggctccgcc cccctgacga
2341 acatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
2401 ccaggcgttt ccccctgatg gctccctctt gcgctctcct gttcccgtcc tgcggcgtcc
2461 gtgttgtggt ggaggcttta cccaaatcac cacgtcccgt tccgtgtaga cagttcgctc
2521 caagctgggc tgtgtgcaag aaccccccgt tcagcccgac tgctgcgcct tatccggtaa
2581 ctatcatctt gagtccaacc cggaaagaca cgacaaaacg ccactggcag cagccattgg
2641 taactgagaa ttagtggatt tagatatcga gagtcttgaa gtggtggcct aacagaggct
2701 acactgaaag gacagtattt ggtatctgcg ctccactaaa gccagttacc aggttaagca
2761 gttccccaac tgacttaacc ttcgatcaaa ccgcctcccc aggcggtttt ttcgtttaca
2821 gagcaggaga ttacgacgat cgtaaaagga tctcaagaag atcctttacg gattcccgac
2881 accatcactc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
2941 catacgatat aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg gaaggagctg
3001 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
3061 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
3121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattga catgttcttt
3181 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
3241 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
3301 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca
3361 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
3421 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
3481 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
3541 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
3601 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
3661 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
3721 ttcctgtttg gtcacttgat gcctccgtgt aagggggaat ttctgttcat gggggtaatg
3781 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
3841 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
3901 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
3961 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
4021 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
4081 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
4141 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
4201 cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac
4261 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
4321 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
4381 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
4441 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg
4501 tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa
4561 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
4621 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
4681 acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct
4741 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
4801 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
4861 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
4921 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
4981 ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca
5041 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc tcgatggcta
5101 cgagggcaga cagtaagtgg atttaccata atcccttaat tgtacgcacc gctaaaacgc
5161 gttcagcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc taaaaactgc
5221 gcgatcgcgc ctgataaatt ttaaccgtat gaatacctat gcaaccagag ggtacaggcc
5281 acattacccc cacttaatcc actgaagctg ccatttttca tggtttcacc atcccagcga
5341 agggccatgc atgcatcgaa attaatacga cgaaattaat acgactcact atagggcaat
5401 tgcgatcacc acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc
5461 aatggcccat tttcctgtca gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg
5521 tcgtagggag accacaacgg tttccctcta gaaataattt tc
//
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