| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001730 |
| 产品用途: | 3' AAVLINK plasmid for MAP1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MAP1A |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-MAP1A-C-FLAG 6915 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6915)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6915
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3619
/codon_start=1
/label=MAP1A
/translation="GAREQPEKEAQSPSPPHPIPMGSPTLWPETEAHVSPPLDSHLGPA
RPSLDFPASAFGFSSLQPAPPQLPSPAEPRSAPCGSLAFSGDRALALAPGPPTRTRHDE
YLEVTKAPSLDSSLPQLPSPSSPGAPLLSNLPRPASPALSEGSSSEATTPVISSVAERF
SPSLEAAEQESGELDPGMEPAAHSLWDLTPLSPAPPASLDLALAPAPSLPGDMGDGILP
CHLECSEAATEKPSPFQVPSEDCAANGPTETSPNPPGPAPAKAENEEAAACPAWERGAW
PEGAERSSRPDTLLSPEQPVCPAGGSGGPPSSASPEVEAGPQGCATEPRPHRGELSPSF
LNPPLPPSIDDRDLSTEEVRLVGRGGRRRVGGPGTTGGPCPVTDETPPTSASDSGSSQS
DSDVPPETEECPSITAEAALDSDEDGDFLPVDKAGGVSGTHHPRPGHDPPPLPQPDPRP
SPPRPDVCMADPEGLSSESGRVERLREKEKVQGRVGRRAPGKAKPASPARRLDLRGKRS
PTPGKGPADRASRAPPRPRSTTSQVTPAEEKDGHSPMSKGLVNGLKAGPMALSSKGSSG
APVYVDLAYIPNHCSGKTADLDFFRRVRASYYVVSGNDPANGEPSRAVLDALLEGKAQW
GENLQVTLIPTHDTEVTREWYQQTHEQQQQLNVLVLASSSTVVMQDESFPACKIEF"
misc_feature 1589..1594
/label=SDS
misc_feature 1986..1991
/label=SDS
misc_feature 2891..2896
/label=SDS
CDS 3623..3688
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3704..4135
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3848..3853
/label=SDS
repeat_region 4178..4318
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4178..4307
/label=AAV2 ITR
rep_origin 4393..4848
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5130..5234
/gene="bla"
/label=AmpR promoter
CDS 5235..6095
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6266..6854
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggg ctcgggaaca
1561 gccagaaaaa gaggcccaat ccccaagtcc tcctcacccc attcctatgg ggtcccccac
1621 attatggcca gaaactgagg cacatgttag ccctcccttg gactcacacc tggggcctgc
1681 ccgacccagt ctggacttcc ctgcttcagc ctttggcttc tcctcattgc agccagctcc
1741 cccacagctg ccctctccag ctgaaccccg ctcggcaccc tgtggctccc ttgccttctc
1801 tggggatcga gctctggctc tggctccagg accccccacc agaacccggc atgatgaata
1861 cctggaagtg accaaggccc ccagcctgga ttcctcactg ccccagctcc catcacccag
1921 ttctcctggg gcccctctcc tctccaatct gccacgacct gcctcaccag ccctgtctga
1981 gggctcctcc tctgaggcta ccacgcctgt gatttcaagt gtggcggagc gcttctctcc
2041 aagccttgag gctgcagaac aggagtctgg agagctggac ccaggaatgg aaccagctgc
2101 ccacagcctc tgggacctca ctcctctgag cccagcaccc ccagcttcac tggacttggc
2161 cctagctcca gctccaagcc tgcctggaga catgggtgat ggcatcctgc cgtgccacct
2221 ggagtgctca gaggcagcca cggagaagcc aagccccttc caggttccct ctgaggattg
2281 tgcagccaat ggcccaactg aaaccagccc taacccccca ggccctgccc cagccaaggc
2341 tgaaaatgaa gaggctgcgg cttgccctgc ctgggaacgt ggggcctggc ctgaaggagc
2401 tgagaggagc tcccggcctg acacattgct ctcccctgag cagccagtgt gtcctgcagg
2461 gggctccggg ggcccaccca gcagtgcctc tcctgaggtc gaagctgggc cccagggatg
2521 tgccactgag cctcggcccc atcgtgggga gctctcccca tccttcctga acccacctct
2581 gcccccatcc atagatgata gggacctctc aactgaggaa gttcggctag taggaagagg
2641 ggggcggcgc cgggtagggg ggccagggac cactgggggc ccatgccctg tgactgatga
2701 gacaccccct acatcagcca gtgactcagg ctcctcacag tcagattctg atgtcccgcc
2761 agaaactgag gagtgtccgt ccatcacagc tgaggcagcc ctcgactcag atgaagatgg
2821 agacttccta cctgtggaca aagctggggg tgtcagtggt actcaccacc ccaggcctgg
2881 ccatgaccca cctcctctcc cacagccaga cccccgccca tcccctcccc gccctgatgt
2941 gtgcatggct gaccccgagg ggctcagctc agagtctggg agagtagaga ggctacggga
3001 gaaggaaaag gttcaggggc gagtagggcg cagggcccca ggcaaggcca agccagcgtc
3061 ccctgcacgg cgtctggatc ttcggggaaa acgctcaccc acccctggta aagggcctgc
3121 agatcgagca tcccgggccc cacctcgacc acgcagcacc acaagccagg tcaccccagc
3181 agaggaaaag gatggacaca gccccatgtc caaaggccta gtcaatggac tcaaggcagg
3241 accaatggcc ttgagttcca agggcagctc tggtgcccct gtatatgtgg atctcgccta
3301 catcccgaat cattgcagtg gcaagactgc tgaccttgac ttcttccgtc gagtgcgtgc
3361 atcctactat gtggtcagtg ggaatgaccc tgccaatggc gagccaagcc gggctgtgct
3421 ggatgccctg ctggagggca aggcccagtg gggggagaat cttcaggtga ctctgatccc
3481 tactcatgac acggaggtga ctcgtgagtg gtaccaacaa actcatgagc agcagcaaca
3541 actgaatgtc ctggtcctgg ctagcagcag caccgtggtg atgcaggatg agtccttccc
3601 tgcctgcaag attgagttca aggactacaa agaccatgac ggtgattata aagatcatga
3661 catcgactac aaggatgacg atgacaagta agaattctga tgactcgaga taatcaacct
3721 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
3781 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
3841 attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc
3901 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt
3961 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat
4021 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
4081 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc
4141 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
4201 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
4261 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
4321 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
4381 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
4441 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
4501 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
4561 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
4621 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
4681 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
4741 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
4801 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
4861 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
4921 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
4981 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
5041 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
5101 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
5161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
5221 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
5281 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
5341 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
5401 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
5461 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
5521 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
5581 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
5641 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
5701 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
5761 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
5821 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
5881 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
5941 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
6001 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
6061 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
6121 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
6181 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
6241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
6301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
6361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
6421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
6481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
6541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
6601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
6661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
6721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
6781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
6841 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
6901 cttttgctca catgt
//
