RCASBP-AP (A) (CC#37)

LOCUS       RCASBP-AP (A) (CC#37)         13699 bp ds-DNA     circular SYN 14-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    RCASBP-AP (A) (CC#37)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Fekete DM, Cepko CL
  TITLE     Replication-competent retroviral vectors encoding alkaline 
            phosphatase reveal spatial restriction of viral gene 
            expression/transduction in the chick embryo.
  JOURNAL   Mol Cell Biol. 1993 Apr . 13(4):2604-13.
  PUBMED    8455633
REFERENCE   2  (bases 1 to 13699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 14, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..262
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             7377..8894
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLGPCMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAA
                     KKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKT
                     YNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGK
                     SVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVIL
                     GGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLD
                     PSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHG
                     HHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGL
                     APGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGED
                     VAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     promoter        9469..9730
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(10320..10342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10331..10347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10355..10385)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(10428..10446)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10514..10618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10619..11479
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10837..10856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11650..12238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12139..12158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12392..12409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12424..12564
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12490..12509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12650..12672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12666..12857)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
      241 attcaccaca ttggtgtgca cctgggttga tggccggacc gttgattccc tgacgactac
      301 gagcacctgc atgaagcaga aggcttcatt tggtgacccc gacgtgatag ttagggaata
      361 gtggtcggcc acagacggcg tggcgatcct gtctccatcc gtctcgtcta tcgggaggcg
      421 agttcgatga ccctggtgga gggggctgcg gcttagggag gcagaagctg agtaccgtcg
      481 gagggagctc cagggcccgg agcgactgac ccctgccgag aactcagagg gtcgtcggaa
      541 gacggagagt gagcccgacg accaccccag gcacgtcttt ggtcggcctg cggatcaagc
      601 atggaagccg tcattaaggt gatttcgtcc gcgtgtaaaa cctattgcgg gaaaatctct
      661 ccttctaaga aggaaatagg ggccatgttg tccctgttac aaaaggaagg gttgcttatg
      721 tctccctcag atttatattc tccggggtcc tgggatccca tcactgcggc gctctcccag
      781 cgggcaatgg tacttggaaa atcgggagag ttaaaaacct ggggattggt tttgggggca
      841 ttgaaggcgg ctcgagagga acaggttaca tctgagcaag caaagttttg gttgggatta
      901 gggggaggga gggtctctcc cccaggtccg gagtgcatcg agaaaccagc tacggagcgg
      961 cgaatcgaca aaggggagga ggtgggagaa acaactgtgc agcgagatgc gaagatggcg
     1021 ccagaggaag cggccacacc taaaaccgtt ggcacatcct gctatcattg cggaacagct
     1081 gttggctgca attgcgccac cgccacagcc tcggcccctc ctccccctta tgtggggagt
     1141 ggtttgtatc cttccctggc gggggtggga gagcagcagg gccagggaga taacacgtct
     1201 cggggggcgg agcagccaag ggaggagcca gggcacgcgg gtcaggcccc tgggccggcc
     1261 ctgactgact gggcaagggt aagggaggag cttgcgagta ctggtccgcc cgtggtggcc
     1321 atgcctgtag tgattaagac agagggaccc gcctggaccc ctctggagcc aaaattgatc
     1381 acaagactgg ctgatacggt caggaccaag ggcttacgat ccccgatcac tatggcagaa
     1441 gtggaagcgc tcatgtcctc cccgttgctg ccgcatgacg tcacgaatct aatgagagtg
     1501 attttaggac ctgccccata tgccttatgg atggacgctt ggggagtcca actccagacg
     1561 gttatagcgg cagccactcg cgacccccga cacccagcga acggtcaagg gcggggggaa
     1621 cggactaact tggatcgatt aaagggctta gctgatggga tggtgggcaa cccacagggt
     1681 caggccgcat tattaagacc gggggaattg gttgctatta cggcgtcggc tctccaggcg
     1741 tttagagaag ttgcccggct ggcggaacct gcaggtccat gggcggacat cacgcaggga
     1801 ccatctgagt cctttgttga ttttgccaat cggcttataa aggcggttga ggggtcagat
     1861 ctcccgcctt ccgcgcgggc tccggtgatc attgactgct ttaggcagaa gtcacagcca
     1921 gatattcagc agcttatacg ggcagcaccc tccacgctga ccaccccagg agagataatc
     1981 aaatatgtgc tagacaggca gaagattgcc cctcttacgg atcaaggcat agccgcggcc
     2041 atgtcgtctg ctatccagcc cttagttatg gcagtagtca atagagagag ggatggacaa
     2101 actgggtcgg gtggtcgtgc ccgagggctc tgctacactt gtggatcccc gggacattat
     2161 caggcacagt gcccgaaaaa acgaaagtca ggaaacagcc gtgagcgatg tcagctgtgt
     2221 gacgggatgg gacacaacgc taaacagtgt aggaagcggg atggcaacca gggccaacgc
     2281 ccaggaagag gtctctcttc ggggccgtgg cccggccctg agcagcctgc cgtctcgtta
     2341 gcgatgacaa tggaacataa agatcgcccc ttggttaggg tcattctgac taacactggg
     2401 agtcatccag tcaaacaacg ttcggtgtat atcaccgcgc tgttggactc cggagcggac
     2461 atcactatta tttcggagga ggattggcct actgattggc cggtggtgga caccgcgaac
     2521 ccacagatcc atggcatagg agggggaatt cccatgcgaa aatcccggga tatgatagag
     2581 gtgggggtta ttaaccgaga cgggtcgttg gagcgacccc tgctcctctt ccccgcagtc
     2641 gctatggtta gagggagtat cctaggaaga gattgtctgc agggcctagg gctccgcttg
     2701 acaaatttat agggagggcc actgttctta ctgttgcgct acatctggct attccgctca
     2761 aatggaagtc agaccgcacg cctgtgtgga ttgaccagtg gccccttcct gaaggtaaac
     2821 ttgtagcgct aacgcaatta gtggaaaaag aattacagtt aggacatata gagccctcac
     2881 ttagttgttg gaacacacct gtttttgtga tccggaaggc ttccgggtct tatcgcttat
     2941 tgcatgattt gcgcgctgtt aacgccaagc ttgttccttt tggggccgtc caacaggggg
     3001 cgccagttct ctccgcgctc ccgcgtggct ggcccctgat ggtcctagac ctcaaggatt
     3061 gcttcttttc tatccctctt gcggaacaag atcgcgaagc ttttgcattt acgctcccct
     3121 ctgtgaataa ccaggccccc gctcgaagat tccaatggaa ggtcttgccc caagggatga
     3181 cctgttctcc cactatctgt cagttggtag tgggtcaggt gctcgagccc ttgcgactca
     3241 agcacccagc tctgcgcatg ttgcattata tggacgatct tttgctagcc gcctcaagtc
     3301 atgatgggtt ggaagcggca gggaaggagg ttatcggtac attggaaaga gccgggttca
     3361 ctatttcgcc ggataagatc cagagggagc ccggagtaca atatcttggg tacaagttag
     3421 gcagtacgta tgtagcaccc gtaggcttgg tagcagaacc caggatagcc accttgtggg
     3481 atgttcaaaa gctggtgggg tcacttcagt ggcttcgccc agcgttaggg atcccgccac
     3541 gactgatggg tcccttttat gagcagttac gagggtcaga tcctaacgag gcgagggaat
     3601 ggaatctaga catgaaaatg gcctggagag agatcgtaca gcttagcact actgctgcct
     3661 tggaacgatg ggaccctgcc cagcctctgg aaggagcggt cgctagatgt gaacaggggg
     3721 caataggggt cctgggacag ggactgtcca cacacccaag gccatgtttg tggttattct
     3781 ccacccaacc caccaaggcg tttactgctt ggttagaagt gctcaccctt ttgattacta
     3841 agctacgcgc ttcggcagtg cgaacctttg gcaaggaggt tgatatcctc ctgttgcctg
     3901 catgcttccg ggaggacctt ccgctcccgg aggggatcct gttagcactt agggggtttg
     3961 caggaaaaat caggagtagt gacacgccat ctatttttga cattgcgcgt ccactgcatg
     4021 tttctctgaa agtgagggtt accgaccacc ctgtgccggg acccactgtc tttaccgacg
     4081 cctcctcaag cacccataaa ggggtggtag tctggaggga gggcccaagg tgggagataa
     4141 aagaaatagt tgatttgggg gcaagtgtac aacaactgga ggcacgcgct gtggccatgg
     4201 cacttctgct gtggccgaca acgcccacta atgtagtgac tgactctgcg tttgttgcga
     4261 aaatgttact caagatggga caggagggag tcccgtctac agcggcagct tttattttag
     4321 aggatgcgtt aagccaaagg tcagccatgg ccgccgttct ccacgtgcgg agtcattctg
     4381 aagtgccagg gtttttcaca gaaggaaatg acgtggcaga tagccaagcc acctttcaag
     4441 cgtatccctt gagagaggct aaagatcttc ataccgctct ccatattgga ccccgcgcgc
     4501 tatccaaagc gtgtaatata tctatgcagc aggctaggga ggttgttcag acctgcccgc
     4561 attgtaattc agcccctgcg ttggaggccg gggtaaaccc taggggtttg ggacccctac
     4621 agatatggca gacagacttt acgcttgagc ctagaatggc tccccgttcc tggctcgctg
     4681 ttactgtgga caccgcctca tcagcgatag tcgtaactca gcatggccgt gttacatcgg
     4741 ttgctgcaca acatcattgg gccacggcta tcgccgtttt gggaagacca aaggccataa
     4801 aaacagataa cgggtcctgc ttcacgtcta aatccacgcg ggagtggctc gcgagatggg
     4861 ggatagcaca caccaccggg attccgggaa attcccaggg tcaagctatg gtagagcggg
     4921 ccaaccggct cctgaaagat aagatccgtg tgcttgcgga aggggacggc tttatgaaaa
     4981 gaatccccgc cagcaaacag ggggaactac tagccaaagc aatgtatgcc ctcaatcact
     5041 ttgagcgtgg tgaaaacaca aaaacaccgg tacaaaaaca ctggagacct accgttctta
     5101 cagaaggacc cccggttaaa atacgaatag agacagggga gtgggaaaaa ggatggaacg
     5161 tgctggtctg gggacgaggt tatgccgctg tgaaaaacag ggacactgat aaggttattt
     5221 gggtaccctc tcgaaaggtt aaaccggacg tcacccaaaa ggatgaggtg actaagaaag
     5281 atgaggcgag ccctcttttt gcaggcattt ctgactggat accctgggaa ggcgagcaag
     5341 aaggattcca agaagaaacc gctagcaaca agcaagaaag acccggagaa gacacccttg
     5401 ctgccaacga gagttaatta tattctcatt attggtgtca tggtcttgtg tgaggttacg
     5461 ggggtaagag ctgatgtcca cttactcgag cagccaggga acctttggat tacatgggcc
     5521 aaccgtacag gccaaacgga tttctgcctc tctacacagt cagccacctc cccttttcaa
     5581 acatgtttga taggtatccc atcccctatt tccgaaggtg attttaaggg atatgtctct
     5641 gataattgca ccactttgga acctcaccgg ttagtctcga gaggcattcc tggcggccct
     5701 gagaacagca caaccctcac ctatcagaag gtttcatgct tgttgttaaa gctgaatgtt
     5761 tctctgttgg acgagccatc agaactacaa ctgctaggtt cccagtctct ccccaatata
     5821 actaatatta ctcggatccc cagtgtggct ggaggatgca taggctttac cccatacgat
     5881 agtccggctg gtgtctacgg atgggaccgg agagaggtta cacacatcct tctgaccgac
     5941 ccagggaaca atcctttctt tgataaggcc tctaactcct cgaaaccgtt tacagtagtg
     6001 acagcggaca ggcacaatct ctttatgggg agtgagtact gtggtgcata tggctacagg
     6061 ttctgggaaa tgtacaattg ctcccaaatg agacagaatt ggtccatctg tcaggatgtg
     6121 tggggccgag gcccccccga aaattggtgc acaagcacag gaggtacatg ggttaatcaa
     6181 tcaaaggaat ttaatgagac agcgccattc agttttactg tgaactgtac tggcagtaat
     6241 ttgggtaatg tcagcggatg ttgcggagaa ccaatcacga ttctcccacc agaggcgtgg
     6301 gtcgacagca cacaaggtag tttcaccaaa ccaaaagcgc taccacccgc aattttcctc
     6361 atttgtgggg atcgcgcatg gcaaggaatt cccagtcgtc cggtaggggg cccctgctat
     6421 ttaggcaagc ttaccatgtt agcacctaag catacagata ttctcaaggt gcttgtcaat
     6481 tcatcgcgga caggtataag acgtaaacga agcacctcac acctggatga tacatgctca
     6541 gatgaagtgc agctttgggg tcctacagca agaatctttg catctatcct agccccgggg
     6601 gtagcagctg cgcaagcctt aagagaaatt gagagactag cctgttggtc cgttaaacag
     6661 gctaacttga caacatcact cctcggggac ttattggatg atgtcacgag tattcgacac
     6721 gcggtcctgc agaaccgagc ggctattgac ttcttgctcc tagctcacgg ccatggctgt
     6781 gaggacgttg ccggaatgtg ctgtttcaat ttgagtgatc acagtgagtc tatacagaag
     6841 aagttccagc taatgaagga acatgtcaat aagatcggcg tggacagcga cccaattgga
     6901 agttggctgc gaggactatt cgggggaata ggagaatggg ccgttcattt gctgaaagga
     6961 ctgcttttgg ggcttgtagt tattttgttg ctagtagtgt gcctgccttg ccttttgcaa
     7021 atcgtatgcg gtaacatcag aaagatgatt aataactcca tcagctacca cacggaatat
     7081 aagaagctgc aaaaggcctg tgggcagcct gaaagcagaa tagtataagg cagtacatgg
     7141 gtggtggtat agcgcttgcg agtccatcga ggaaggcagg aaagacagct attggtaatt
     7201 gtgaaatacg cttttgtctg tgtgctgcag gagctgagct gactctgctg gtggcctcgc
     7261 gtaccactgt ggcatcgata agcttggctg caggtcgacc cgagctcgga tccactagta
     7321 acggccgcca gtgtgctgga attcctgcct cgccactgtc ctgctgccct ccagacatgc
     7381 tggggccctg catgctgctg ctgctgctgc tgctgggcct gaggctacag ctctccctgg
     7441 gcatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca gccgaggccc
     7501 tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc atcatcttcc
     7561 tgggcgatgg gatgggggtg tctacggtga cagctgccag gatcctaaaa gggcagaaga
     7621 aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat gtggctctgt
     7681 ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc acggcctacc
     7741 tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc cgctttaacc
     7801 agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc aagaaagcag
     7861 ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca gccggcacct
     7921 acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc tcggcccgcc
     7981 aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt gacgtgatcc
     8041 taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag tacccagatg
     8101 actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa tggctggcga
     8161 agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct tccctggacc
     8221 cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac gagatccacc
     8281 gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg cgcctgctga
     8341 gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac catggtcatc
     8401 atgaaagcag ggcttaccgg gcactgactg agacgatcat gttcgacgac gccattgaga
     8461 gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc gaccactccc
     8521 acgtcttctc cttcggaggc taccccctgc gagggagctc catcttcggg ctggcccctg
     8581 gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt ccaggctatg
     8641 tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc cccgagtatc
     8701 ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac gtggcggtgt
     8761 tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc ttcatagcgc
     8821 acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg gcgccccccg
     8881 ccggcaccac cgacgccgcg cacccggggc ggtccgtggt ccccgcgttg cttcctctgc
     8941 tggccgggac cctgctgctg ctggagacgg ccactgctcc ctgagtgtcc cgtccctggg
     9001 gctcctgctt ccccatcccg gagttctcct gctccccacc tcctgtcgtc ctgcctggcc
     9061 tccagcccga gtcgtcatcc ccggagtccc tatacagagg tcctgccatg gaaccttccc
     9121 ctccccgtgc gctctgggga ctgagcccat gacaccaaac ctgccccttg gctgctctcg
     9181 gactccctac cccaacccca gggactgcag gttgtgccct gtggctgcct gcaccccagg
     9241 aaaggagggg gctcaggcca tccagccacc acctacagcc cagtggggtc gactctagag
     9301 gatccccggg cgagctcgaa ttccgagctt atcgatgcga tgtacgggcc agatatacgc
     9361 gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg tacgcggtta
     9421 ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa tgtagtctta
     9481 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     9541 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     9601 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattcc gcattgcaga
     9661 gatattgtat ttaagtgcct agctcgatac aataaacgcc atttgaccat tcaccacatt
     9721 ggtgtgcacc tgggttgatg gccggaccgt tgattccctg acgactacga gcacctgcat
     9781 gaagcagaag gcttcatttg gtgaccccga cgtgatagtt agggaatagt ggtcggccac
     9841 agacggcgtg gcgatcctgt ctccatccgt ctcgtctatc gggaggcgag ttcgatgacc
     9901 ctggtggagg gggctgcggc ttagggaggc agaagctgag taccgtcgga ggggatccac
     9961 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
    10021 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
    10081 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
    10141 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
    10201 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
    10261 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gctccaagct tggctgtttc
    10321 ctgtgtgaaa ttgttatccg ctcacaattc cacacattat acgagccgga agcataaagt
    10381 gtaaagcctg gggtgcctaa tgagtgagaa ttcttgaaga cgaaagggcc tcgtgatacg
    10441 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
    10501 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10561 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10621 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10681 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10741 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10801 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10861 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10921 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10981 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    11041 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    11101 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    11161 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    11221 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    11281 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11341 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11401 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11461 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11521 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11581 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11641 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11701 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11761 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    11821 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11881 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11941 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    12001 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    12061 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12121 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12181 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12241 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12301 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    12361 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    12421 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    12481 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    12541 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    12601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    12661 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
    12721 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
    12781 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
    12841 ttttcctgtt tggtcactga tgcctccgtg taaggaggat ttctgttcat gggggtaatg
    12901 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
    12961 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
    13021 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
    13081 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
    13141 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
    13201 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
    13261 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
    13321 cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac
    13381 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
    13441 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
    13501 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
    13561 cggcgatgcg atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg
    13621 cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc
    13681 ttttgcatag ggaggggga
//