| 质粒名称: | pAAVLINK-EF1a-HA-MAP1A-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001727 |
| 产品用途: | 5' AAVLINK plasmid for MAP1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MAP1A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-MAP1A-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-MAP1A-A载体质粒基本信息
pAAVLINK-EF1a-HA-MAP1A-A质粒图谱载体图谱和pAAVLINK-EF1a-HA-MAP1A-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-MAP1A-A质粒载体简介
pAAVLINK-EF1a-HA-MAP1A-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-MAP1A-A 5663 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5663)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5663
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..2803
/codon_start=1
/label=MAP1A
/translation="DGVAEFSEYVSETVDVPSPFDLLEPPTSGGFLKLSKPCCYIFPGG
RGDSALFAVNGFNILVDGGSDRKSCFWKLVRHLDRIDSVLLTHIGADNLPGINGLLQRK
VAELEEEQSQGSSSYSDWVKNLISPELGVVFFNVPEKLRLPDASRKAKRSIEEACLTLQ
HLNRLGIQAEPLYRVVSNTIEPLTLFHKMGVGRLDMYVLNPVKDSKEMQFLMQKWAGNS
KAKTGIVLPNGKEAEISVPYLTSITALVVWLPANPTEKIVRVLFPGNAPQNKILEGLEK
LRHLDFLRYPVATQKDLASGAVPTNLKPSKIKQRADSKESLKATTKTAVSKLAKREEVV
EEGAKEARSELAKELAKTEKKAKESSEKPPEKPAKPERVKTESSEALKAEKRKLIKDKV
GKKHLKEKISKLEEKKDKEKKEIKKERKELKKDEGRKEEKKDAKKEEKRKDTKPELKKI
SKPDLKPFTPEVRKTLYKAKVPGRVKIDRSRAIRGEKELSSEPQTPPAQKGTVPLPTIS
GHRELVLSSPEDLTQDFEEMKREERALLAEQRDTGLGDKPFPLDTAEEGPPSTAIQGTP
PSVPGLGQEEHVMKEKELVPEVPEEQGSKDRGLDSGAETEEEKDTWEEKKQREAERLPD
RTEAREESEPEVKEDVIEKAELEEMEEVHPSDEEEEDATKAEGFYQKHMQEPLK"
misc_feature 1062..1067
/label=SDS
misc_feature 1203..1208
/label=SDS
misc_feature 1437..1442
/label=SDS
misc_feature 1746..1751
/label=SDS
misc_feature 1770..1775
/label=SDS
misc_feature 2043..2048
/label=SDS
misc_feature 2067..2072
/label=SDS
misc_feature 2427..2432
/label=SDS
misc_feature 2679..2684
/label=SDS
misc_feature 2718..2723
/label=SDS
misc_feature 2739..2744
/label=SDS
intron 2804..2844
/label=Splicing Donor
misc_feature 2848..2881
/label=loxP-JT1571
repeat_region 2926..3066
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2926..3055
/label=AAV2 ITR
rep_origin 3141..3596
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3878..3982
/gene="bla"
/label=AmpR promoter
CDS 3983..4843
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5014..5602
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgacg gcgtggctga gttctccgag tatgtctctg agactgtgga
781 cgtgccatcc ccatttgacc tactagagcc ccccacctca gggggcttcc tcaagctctc
841 caagccttgt tgctacatct tcccaggtgg tcgtggggac tctgccctct ttgctgtcaa
901 tggtttcaac atcctggtgg atggtggctc tgatcgcaag tcctgttttt ggaagctggt
961 acggcacttg gaccgcattg actcggtgct actcacacac attggggcag acaacctgcc
1021 aggcatcaat ggactactgc agcgcaaagt ggcagagcta gaggaggagc agtcccaggg
1081 ctctagcagt tacagcgact gggtgaagaa ccttatctct cctgagcttg gagttgtctt
1141 tttcaacgtg cctgagaagc tgcggcttcc tgatgcctcc cggaaagcca agcgtagcat
1201 tgaggaggcc tgcctcactc tgcagcactt aaaccgcctg ggcatccagg ctgagcctct
1261 atatcgtgtg gtcagcaata ccattgagcc actgaccctc ttccacaaaa tgggtgtggg
1321 ccggctggac atgtatgtcc tcaaccctgt caaggacagc aaggagatgc agttcctcat
1381 gcaaaagtgg gcaggcaata gtaaagccaa gacaggcatc gtgctgccca atgggaagga
1441 ggctgagatc tccgtgccct accttacctc tatcactgct ctggtggtct ggctaccagc
1501 caatcccact gagaagattg tgcgtgtgct ttttccagga aatgctcccc aaaacaagat
1561 cttggagggc ctagaaaagc ttcggcatct ggacttcctg cgttaccctg tggccacgca
1621 gaaggacctg gcttctgggg ctgtgcctac caacctcaag cccagcaaaa tcaaacagcg
1681 ggctgatagc aaggagagcc tcaaagccac taccaagacg gccgtgagca agttggccaa
1741 acgggaggag gtggtagaag agggagccaa ggaggcacgt tcagagctgg ccaaggagtt
1801 agccaagaca gagaagaagg caaaagagtc atctgagaag cccccagaga agcctgccaa
1861 gcctgagagg gtgaagacag agtcaagtga ggcactgaag gcagagaagc gaaagctgat
1921 caaagacaag gtagggaaaa agcaccttaa agaaaagata tcaaagctgg aagaaaaaaa
1981 agacaaggag aaaaaagaga tcaaaaagga gaggaaagag ctcaagaagg atgaaggaag
2041 gaaggaggag aagaaggatg ccaagaagga ggagaagagg aaagatacca aacctgagct
2101 caagaagatt tccaagccag acctaaagcc ctttactcct gaggtacgta agaccctcta
2161 taaagccaag gtccctggaa gagtcaaaat agacaggagc cgtgctatcc gtggggagaa
2221 ggagctgtct tctgagcccc agacaccccc agcccagaag ggaactgtac cactcccaac
2281 catcagtggg cacagggagc tggtcctatc ctcaccagag gacctcacac aggactttga
2341 ggagatgaag cgtgaggaga gggctttgct ggctgaacaa agggacacag gactaggaga
2401 taagccattc cctctagaca ctgcagagga gggaccccca agtacagcta tccagggaac
2461 accaccctct gttccagggc tgggacaaga agaacatgtg atgaaggaga aagagcttgt
2521 cccagaggtc cctgaggaac aaggcagcaa ggacagaggc ctagactctg gggctgaaac
2581 agaggaagag aaagatacct gggaggaaaa gaagcagagg gaagcagaga ggctcccaga
2641 cagaacagaa gccagagagg aaagtgaacc tgaagtaaag gaggatgtga tagaaaaggc
2701 tgagttagaa gaaatggagg aggtacaccc ttcagatgag gaggaagagg acgcgacaaa
2761 agctgagggt ttttaccaaa aacatatgca ggaacccttg aaggtaagta tcaaggttac
2821 aagacaggtt taaggagacc aataagctat tattcgtata gcatacatta tacgaagtta
2881 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
2941 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
3001 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
3061 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
3121 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
3181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
3241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
3301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
3361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
3421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
3481 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
3541 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
3601 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
3661 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
3721 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
3781 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
3841 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
3901 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
3961 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
4021 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
4081 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
4141 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
4201 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
4261 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
4321 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
4381 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
4441 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
4501 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
4561 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
4621 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
4681 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
4741 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
4801 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
4861 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
4921 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
4981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
5041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
5101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
5161 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
5221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
5281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
5341 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
5401 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
5461 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
5521 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
5581 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
5641 ttgctggcct tttgctcaca tgt
//
