GBX

LOCUS       GBX                9809 bp ds-DNA     circular SYN 03-NOV-2022
DEFINITION  a modified episomal (EBNA1/OriP) vector expressing human eGFP and 
            BCL-xL genes.
ACCESSION   .
VERSION     .
KEYWORDS    GBX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, 
            Cheng L
  TITLE     A Facile Method to Establish Human Induced Pluripotent Stem Cells 
            From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture 
            Conditions: A Clinically Compliant Approach.
  JOURNAL   Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
  PUBMED    25742692
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 3, 2022 from SnapGene Server 1.1.58
            http://www.biofrng.com
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             427..1143
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             427..1143
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(472..493)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(733..752)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1080..1101
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1153..1209
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(1981..2001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2399..2410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2507..2641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2531..2550)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2585..2604
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2784..2806)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3366..5155
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5457..7382)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGG?GG?GG?G?GGG?G?GGG?GG?GG?G?GGG?G?GGGGGG?GGGGG?
                     GG?GG?G?GGGAG?GGG?GG?G?GGG?GG?GG?G?GGG?G?GG?GGAGG?G?GG?G?GG
                     G?GG?GG?G?GGAG?GG?G?GGAGAGGAGGAGAGG?GG?G?GG?GG?G?GGG?GG?GAG
                     GG?GG?GAGG?GG?G?GG?GGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7817..7835)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7903..8007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8008..8868
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8226..8245)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9039..9627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9528..9547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggta ccgcgctagc
      421 gccagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      481 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      541 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      601 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      661 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      721 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      781 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      841 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      901 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      961 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1021 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1081 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1141 aagggatccg gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac
     1201 cccggtccta tgtctcagag caaccgggag ctggtggttg actttctctc ctacaagctt
     1261 tcccagaaag gatacagctg gagtcagttt agtgatgtgg aagagaacag gactgaggcc
     1321 ccagaaggga ctgaatcgga gatggagacc cccagtgcca tcaatggcaa cccatcctgg
     1381 cacctggcag acagccccgc ggtgaatgga gccactggcc acagcagcag tttggatgcc
     1441 cgggaggtga tccccatggc agcagtaaag caagcgctga gggaggcagg cgacgagttt
     1501 gaactgcggt accggcgggc attcagtgac ctgacatccc agctccacat caccccaggg
     1561 acagcatatc agagctttga acaggtagtg aatgaactct tccgggatgg ggtaaactgg
     1621 ggtcgcattg tggccttttt ctccttcggc ggggcactgt gcgtggaaag cgtagacaag
     1681 gagatgcagg tattggtgag tcggatcgca gcttggatgg ccacttacct gaatgaccac
     1741 ctagagcctt ggatccagga gaacggcggc tgggatactt ttgtggaact ctatgggaac
     1801 aatgcagcag ccgagagccg aaagggccag gaacgcttca accgctggtt cctgacgggc
     1861 atgactgtgg ccggcgtggt tctgctgggc tcactcttca gtcggaaatg agtttaaacg
     1921 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1981 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2041 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2101 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2161 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2221 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2281 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     2341 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2401 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2461 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggcggatc cagacatgat
     2521 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2581 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2641 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2701 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     2761 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2821 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2881 gggtgtcggg gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac
     2941 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
     3001 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
     3061 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
     3121 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
     3181 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     3241 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     3301 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     3361 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     3421 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     3481 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
     3541 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
     3601 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
     3661 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
     3721 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
     3781 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
     3841 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
     3901 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
     3961 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
     4021 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
     4081 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
     4141 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
     4201 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
     4261 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
     4321 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
     4381 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
     4441 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
     4501 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4561 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     4621 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4681 ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg
     4741 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
     4801 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
     4861 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4921 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4981 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     5041 cctcatgcat atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg
     5101 catatgccgc cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt
     5161 tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc
     5221 tcaccaggta aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag
     5281 tgacgtgaca acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa
     5341 gacagatggc cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta
     5401 gtgcggggga atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac
     5461 tcctgccctt cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca
     5521 ccctccgcgg cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg
     5581 tcaaagctgc acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg
     5641 tccttaatcg catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa
     5701 taacagacaa tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca
     5761 aaggggagac gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc
     5821 cttaggttgt aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt
     5881 ccttcgtcgg tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca
     5941 atgttctcaa atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct
     6001 tttttgcgcc tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg
     6061 tcatctgcgg ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca
     6121 ccttcttggt ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta
     6181 cctggagggg gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg
     6241 ggcctctttt ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg
     6301 gctctttcac gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta
     6361 cctcctcgac cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc
     6421 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6481 cctscccctc ctsctcctsc ccctcctscc cctcctgctc ctscccctcc tscccctcct
     6541 cctgctccts cccctcctsc ccctcctcct sctcctsccc ctcctscccc tcctsctcct
     6601 scccctccts cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctsct
     6661 cctscccctc ctsctcctgc ccctcctsct cctscccctc ctscccctcc tscccctcct
     6721 cctsctccts cccctcctsc tcctscccct cctgcccctc ctscccctcc tsctcctscc
     6781 cctcctccts ctcctscccc tcctscccct cctscccctc ctcctsctcc tscccctcct
     6841 scccctcctc ctsctcctgc ccctcctcct sctcctsccc ctcctscccc tcctscccct
     6901 cctcctcctc ctscccctcc tccccctcct cctsctccts cccctcctcc tsctcctscc
     6961 cctcctsccc ctcctscccc tcctcctsct cctscccctc ctcctsctcc tscccctcct
     7021 scccctccts cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     7081 cctgctcctg cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag
     7141 ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat
     7201 cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct
     7261 cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca
     7321 gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac
     7381 atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag
     7441 ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct
     7501 gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc
     7561 aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc
     7621 aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg
     7681 ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga
     7741 aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat
     7801 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7861 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7921 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7981 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8041 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8101 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8161 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8221 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8281 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8341 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8401 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8461 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8521 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     8581 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8641 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8701 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8761 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8821 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8881 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8941 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9001 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9061 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9181 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9661 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
     9721 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
     9781 atccagcctc gcgtcgcgag ctagctgca
//