| 质粒名称: | QPM-sgR (pTaU3) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 140448 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pMOD_C0000 |
| 载体出品公司: | Daniel Voytas (Addgene plasmid # 91081) |
| 完整质粒大小: | 2866bp |
| 载体类型: | Plant Expression, CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | TaU3-sgRNA |
| 物种来源: | Streptococcus pyogene |
| 启动子: | TaU3 |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | QPM-sgR (pTaU3) was a gift from Caixia Gao (Addgene plasmid # 140448 ; http://n2t.net/addgene:140448 ; RRID:Addgene_140448) |
| 参考文献部分: | Prime genome editing in rice and wheat. Lin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, Wang Y, Anzalone AV, Raguram A, Doman JL, Liu DR, Gao C. Nat Biotechnol. 2020 May;38(5):582-585. doi: 10.1038/s41587-020-0455-x. Epub 2020 Mar 16. 10.1038/s41587-020-0455-x PubMed 32393904 |
买家导航
QPM-sgR (pTaU3)载体质粒基本信息
QPM-sgR (pTaU3)质粒图谱载体图谱和QPM-sgR (pTaU3)载体序列质粒序列多克隆位点信息
质粒图谱.png)
QPM-sgR (pTaU3)质粒载体简介
QPM-sgR (pTaU3)质粒序列载体序列
LOCUS QPM-sgR (pTaU3) 2866 bp DNA circular SYN 25-AUG-2024
DEFINITION For sgRNA expression in monocotyledons protoplasts.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2866)
AUTHORS Lin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, Wang Y, Anzalone AV,
Raguram A, Doman JL, Liu DR, Gao C
TITLE Prime genome editing in rice and wheat.
JOURNAL Nat Biotechnol. 2020 May;38(5):582-585. doi:
10.1038/s41587-020-0455-x. Epub 2020 Mar 16.
PUBMED 32393904
REFERENCE 2 (bases 1 to 2866)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41587-020-0455-x"; journalName: "Nat Biotechnol"; date:
"2020-05"; volume: "38"; issue: "5"; pages: "582-585"
FEATURES Location/Qualifiers
source 1..2866
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_RNA 425..500
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 511..527
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 511..527
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 540..714
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
promoter 859..963
/gene="bla"
/label=AmpR promoter
rep_origin 2031..2619
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2520..2539
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2773..2790
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ggcggcaggg agagttttaa cattgactag cgtgctgata atttgtgaga aataataatt
61 gacaagtaga tactgacatt tgagaagagc ttctgaactg ttattagtaa caaaaatgga
121 aagctgatgc acggaaaaag gaaagaaaaa gccatacttt tttttaggta ggaaaagaaa
181 aagccatacg agactgatgt ctctcagatg ggccgggatc tgtctatcta gcaggcagca
241 gccctaccaa cctcacgggc cagcaattac gagtccttct aaaacgtccc gccgagggcg
301 cgtggccgtg ctgtgcagca gcacgtctaa cattagtccc acctcgccag tttacaggga
361 gcagaaccag cttataagcg gaggcgcggc accaagaagc gagagacgga gtcgattcgt
421 ctcggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
481 aagtggcacc gagtcggtgc tttttttttt caggaaacag ctatgaccca tggctcgagt
541 ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt
601 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
661 tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga
721 attagagctc gcgcgtagtc ctcggtaata tcgcagaaca aaagtacctg atatcgagtg
781 tacttcaagt cacaccggcg agtgactagc aggtgatgaa aagccttagg gtcaggtggc
841 acttttcggg gaaatgtgcg atgaacccct atttgtttat ttttctaaat acattcaaat
901 atgtatccgc taatgagaca ataaccctga taaatgcttc aattatattg aaaaaggaag
961 agtatgagta tccaacattt ccgtgtcgcc cttattccct tttttgcggc cttctgcctt
1021 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
1081 gcccgagtgg gttacattga actggatctc aacagcggca aaatcttaga gagttttcgc
1141 cccgaagaac gcttcccaat gatgagcact ttcaaagttc tgctatgtgg cgcggtgtta
1201 tcccgtattg atgctgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
1261 ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
1321 ttatgtagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
1381 attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
1441 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
1501 atgcctgtag caatggcaac aactctccgc aaactattaa ctggcgaact acttactctt
1561 gcttcacgcc agcaactcat tgactggatg gaggcggaca aagttgcagg accacttctg
1621 cgctcggcac ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc
1681 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
1741 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
1801 gcctcactga ttaagcattg gtaactgtca gacgaaggga aataaatagt agcccgcctg
1861 atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt aggtcctctt
1921 aggcggtccg attagcgaca tagtcatcaa tgccttgacg gcaaaatccc ttaacgtgag
1981 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
2041 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
2101 tgtttgccgg atcaagagct actaactctt tttccgaagg taactggctt cagcagagcg
2161 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
2221 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
2281 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
2341 tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac ctacaccgaa
2401 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
2461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
2521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
2581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
2641 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
2701 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
2761 acgaccgagc gcagcgagtc agtgagcgag gaagcgggag agcgcccacc tgccagggca
2821 gcgaagagca agccagataa cagtatgcgt atttgcgcgc ttcgaa
//
