pCDNA3.1-Fluc

LOCUS       pCDNA3.1-Fluc               12047 bp DNA     circular SYN 16-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        913..943
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             2852..3032
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     3538..3554
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3557..4145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3663..3674
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4170..4769)
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     misc_feature    4770..5335
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(5345..6997)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      6994..7003
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7051..8262)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     misc_feature    8327..8444
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8742..8786)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8971..9204
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9697..9822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9869..10049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10067..10265)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        10267..10646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10912..11016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11017..11877
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc
     3061 atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca
     3121 tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct
     3181 tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
     3241 ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag
     3301 atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     3361 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     3421 acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa
     3481 tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga
     3541 ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     3601 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     3661 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     3721 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     3781 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     3841 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     3901 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     3961 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     4021 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     4081 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     4141 tgattatcga taagcttgat atcgaattct caggcaccgg gcttgcgggt catgcaccag
     4201 gtcgcgcggt ccttcgggca ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
     4261 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
     4321 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     4381 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     4441 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     4501 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     4561 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     4621 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     4681 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     4741 aggcgcaccg tgggcttgta ctcggtcatt tatcatcgtg tttttcaaag gaaaaccacg
     4801 tccccgtggt tcggggggcc tagacgtttt ttaacctcga ctaaacacat gtaaagcatg
     4861 tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc
     4921 ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa
     4981 cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc
     5041 agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg
     5101 tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca
     5161 ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt
     5221 tccgggccct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
     5281 cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg agggagctag
     5341 cttattacac ggcgatcttt ccgcccttct tggcctttat gaggatctct ctgatttttc
     5401 ttgcgtcgag ttttccggta agacctttcg gtacttcgtc cacaaacaca actcctccgc
     5461 gcaacttttt cgcggttgtt acttgactgg cgacgtaatc cacgatctct ttttccgtca
     5521 tcgtctttcc gtgctccaaa acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg
     5581 gaagacctgc gacacctgcg tcgaagatgt tggggtgttg gagcaagatg gattccaatt
     5641 cagcgggagc cacctgatag cctttgtact taatcagaga cttcaggcgg tcaacgatga
     5701 agaagtgttc gtcttcgtcc cagtaagcta tgtctccaga atgtagccat ccatccttgt
     5761 caatcaaggc gttggtcgct tccggattgt ttacataacc ggacataatc ataggacctc
     5821 tcacacacag ttcgcctctt tgattaacgc ccagcgtttt cccggtatcc agatccacaa
     5881 ccttcgcttc aaaaaatgga acaactttac cgaccgcgcc cggtttatca tccccctcgg
     5941 gtgtaatcag aatagctgat gtagtctcag tgagcccata tccttgcctg atacctggca
     6001 gatggaacct cttggcaacc gcttccccga cttccttaga gaggggagcg ccaccagaag
     6061 caatttcgtg taaattagat aaatcgtatt tgtcaatcag agtgcttttg gcgaagaagg
     6121 agaatagggt tggcaccagc agcgcacttt gaatcttgta atcctgaagg ctcctcagaa
     6181 acagctcttc ttcaaatcta tacattaaga cgactcgaaa tccacatatc aaatatccga
     6241 gtgtagtaaa cattccaaaa ccgtgatgga atggaacaac acttaaaatc gcagtatccg
     6301 gaatgatttg attgccaaaa ataggatctc tggcatgcga gaatctcacg caggcagttc
     6361 tatgaggcag agcgacacct ttaggcagac cagtagatcc agaggagttc atgatcagtg
     6421 caattgtctt gtccctatcg aaggactctg gcacaaaatc gtattcatta aaaccgggag
     6481 gtagatgaga tgtgacgaac gtgtacatcg actgaaatcc ctggtaatcc gttttagaat
     6541 ccatgataat aattttttgg atgattggga gctttttttg cacgttcaaa attttttgca
     6601 accccttttt ggaaacgaac accacggtag gctgcgaaat gcccatactg ttgagcaatt
     6661 cacgttcatt ataaatgtcg ttcgcgggcg caactgcaac tccgataaat aacgcgccca
     6721 acaccggcat aaagaattga agagagtttt cactgcatac gacgattctg tgatttgtat
     6781 tcagcccata tcgtttcata gcttctgcca accgaacgga catttcgaag tactcagcgt
     6841 aagtgatgtc cacctcgata tgtgcatctg taaaagcaat tgttccagga accagggcgt
     6901 atctcttcat agccttatgc agttgctctc cagcggttcc atcttccagc ggatagaatg
     6961 gcgccgggcc tttctttatg tttttggcgt cttccatggt ggcgaccggt acccggggat
     7021 cctctagagt cgacctgcag cccaagcttc gtctaacaaa aaagccaaaa acggccagaa
     7081 tttagcggac aatttactag tctaacactg aaaattacat attgacccaa atgattacat
     7141 ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc aaccccgagc gcatagttca
     7201 aaaccggagc ttcagctact taagaagata ggtacataaa accgaccaaa gaaactgacg
     7261 cctcacttat ccctcccctc accagaggtc cggcgcctgt cgattcagga gagcctaccc
     7321 taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac cgcacaccta ccggcaggtg
     7381 gccccaccct gcattataag ccaacagaac gggtgacgtc acgacacgac gagggcgcgc
     7441 gctcccaaag gtacgggtgc actgcccaac ggcaccgcca taactgccgc ccccgcaaca
     7501 gacgacaaac cgagttctcc agtcagtgac aaacttcacg tcagggtccc cagatggtgc
     7561 cccagcccat ctcacccgaa taagagcttt cccgcattag cgaaggcctc aagaccttgg
     7621 gttcttgccg cccaccatgc cccccacctt gtttcaacga cctcacagcc cgcctcacaa
     7681 gcgtcttcca ttcaagactc gggaacagcc gccattttgc tgcgctcccc ccaaccccca
     7741 gttcagggca accttgctcg cggacccaga ctacagccct tggcggtctc tccacacgct
     7801 tccgtcccac cgagcggccc ggcggccacg aaagccccgg ccagcccagc agcccgctac
     7861 tcaccaagtg acgatcacag cgatccacaa acaagaaccg cgacccaaat cccggctgcg
     7921 acggaactag ctgtgccaca cccggcgcgt ccttatataa tcatcggcgt tcaccgcccc
     7981 acggagatcc ctccgcagaa tcgccgagaa gggactactt ttcctcgcct gttccgctct
     8041 ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg tcctaaaatg tccttctgct
     8101 gatactgggg ttctaaggcc gagtcttatg agcagcgggc cgctgtcctg agcgtccggg
     8161 cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc gccgtgagga
     8221 ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatctgg agccgacacg
     8281 ggttaattaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     8341 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     8401 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
     8461 ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg
     8521 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
     8581 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
     8641 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
     8701 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
     8761 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
     8821 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
     8881 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
     8941 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
     9001 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
     9061 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
     9121 tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc
     9181 aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt
     9241 ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat
     9301 aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc
     9361 ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     9421 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     9481 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     9541 acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct
     9601 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     9661 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     9721 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     9781 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     9841 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     9901 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     9961 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
    10021 gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc ttatatagac
    10081 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
    10141 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
    10201 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
    10261 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
    10321 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
    10381 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
    10441 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
    10501 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
    10561 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
    10621 ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag
    10681 cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt
    10741 tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag
    10801 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc
    10861 gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    10921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    10981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    11041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    11101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    11161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    11221 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    11281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    11341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    11401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    11461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    11521 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    11581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    11641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    11701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    11761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    11821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    11881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    11941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    12001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//