pDisplay-Asp-RGEPO1

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

买家导航

pDisplay-Asp-RGEPO1载体质粒基本信息

质粒名称: pDisplay-Asp-RGEPO1
出品公司: Wekwikgene
目录编号: 0000767
产品用途: Expressing the red potassium indicator on the cell surface of mammalian cells
存储实验室: KIRYL PIATKEVICH Lab

载体骨架基本信息
载体名称: pDisplay
表达系统: 哺乳动物表达
启动子: CMV
拷贝数: 未拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: TOP10

插入基因信息
插入基因名称: Asp-RGEPO1
物种来源: bacterium
密码子优化: 哺乳细胞表达优化

文献引用方法
参考文献部分:
Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.
Yang L., Pathiranage V., Zhou S., Sun X., Zhang H., Lai C., Gu C., Subach F.V., Drobizhev M., Walker A.R., Piatkevich K.D.
PLOS Biol. 23, e3002993 (2025)

pDisplay-Asp-RGEPO1质粒图谱载体图谱和pDisplay-Asp-RGEPO1载体序列质粒序列多克隆位点信息

pDisplay-Asp-RGEPO1载体图谱

pDisplay-Asp-RGEPO1质粒载体简介

pDisplay-Asp-RGEPO1质粒序列载体序列

LOCUS       pDisplay-Asp-RGEPO1               6471 bp DNA     circular SYN 23-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Kiryl Piatkevich
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        466..845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        846..1049
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1094..1112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1118..1137
                     /label=pDisplay-F
     CDS             1193..1264
                     /codon_start=1
                     /label=Asp-tag
                     /translation="MASSLYSFLLALSIVYIFVAPTHS"
     CDS             1265..1291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     1287..1331
                     /label=PD-F1
     CDS             1313..1765
                     /codon_start=1
                     /label=N-mApple
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
                     EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
     primer_bind     1739..1786
                     /label=FR-1-3-F
     primer_bind     1739..1760
                     /label=FR-Y-Ra-F
     primer_bind     complement(1748..1768)
                     /label=mApple-N-R1
     primer_bind     complement(1752..1765)
                     /label=N-mapple-R
     primer_bind     complement(1754..1772)
                     /label=FR-1-3-R
     CDS             1766..1768
                     /codon_start=1
                     /label=LINKER1
                     /translation="P"
     primer_bind     complement(1767..1816)
                     /label=F6A-R1
     CDS             1769..2200
                     /codon_start=1
                     /label=YgaU_Hydrothermal
                     /translation="GIWDFVKSAGRKIGIGSEEAAPAADDLKKELDSYKLGTDDVKVEV
                     VGDKAVITGNVADQSILEKAIVAVGNTLGISKVESNVEVPDAKEPVFHTVKKGDTLWAI
                     ASKQYGNVSKYNAIFEANKPMLSHPDKIYPGQVLRIPDLG"
     primer_bind     complement(1772..1816)
                     /label=V7A-R1
     primer_bind     complement(1775..1816)
                     /label=K8A-R1
     primer_bind     complement(1779..1816)
                     /label=S9A-R1
     misc_feature    1781..1801
                     /label=Mutant
     primer_bind     complement(1786..1816)
                     /label=G11A-R1
     primer_bind     complement(1786..1816)
                     /label=R12A-R1
     primer_bind     1802..1819
                     /label=0811-F2
     primer_bind     complement(1963..2014)
                     /label=G71A-R1
     primer_bind     complement(1964..2014)
                     /label=N72A-R1
     primer_bind     complement(1967..2014)
                     /label=T73A-R1
     misc_feature    1976..1999
                     /label=Mmutant
     primer_bind     complement(1978..2014)
                     /label=G75A-R1
     primer_bind     complement(1979..2014)
                     /label=I76A-R1
     primer_bind     complement(1981..2014)
                     /label=S77A-R1
     primer_bind     complement(1986..2015)
                     /label=K78A-R1
     primer_bind     complement(1988..2014)
                     /label=L74A-R1
     primer_bind     2000..2018
                     /label=0811-F1
     primer_bind     complement(2092..2116)
                     /label=G113V-R
     primer_bind     complement(2177..2193)
                     /label=Ec-kbp-R
     primer_bind     2201..2219
                     /label=mApple-C-F1
     CDS             2201..2209
                     /codon_start=1
                     /label=LINKER
                     /translation="SAC"
     CDS             2210..2467
                     /codon_start=1
                     /label=C-mApple
                     /translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
                     GAYIVDIELDILSHNVDYTIVEQCERAVGRHPTGGTDALYK"
     primer_bind     2210..2230
                     /label=C-mApple-F2
     primer_bind     complement(2400..2419)
                     /label=FR-R
     primer_bind     complement(2449..2488)
                     /label=PD-R1
     primer_bind     2468..2488
                     /label=MYC-F1
     CDS             2477..2506
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2510..2656
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(2638..2684)
                     /label=MYC-R1
     polyA_signal    2684..2908
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3040..3628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3957..4004
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4236..5030)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5478..6338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6339..6443)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
       61 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
      361 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      421 gcgaatttta acaaaatatt aacgcttaca atttacgcgc gcgttgacat tgattattga
      481 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      541 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      601 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      661 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      721 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      781 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      841 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      901 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      961 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     1021 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     1081 ggcttatcga aattaatacg actcactata gggagaccca agcttggtac cgagctcgga
     1141 tccactagta acggccgcca gtgtgctgga attcggcttg gggatatcca ccatggccag
     1201 cagcctgtac agcttcctgc tggccctgag catcgtgtac atcttcgtgg cccccaccca
     1261 cagctatcca tatgatgttc cagattatgc tggggcccag ccggccagat ctatggtgag
     1321 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     1381 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1441 cgaggccttc cagaccgcta ggctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     1501 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacattaagc acccagccga
     1561 catccccgac tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgag
     1621 cttcgaggac ggcggcatta ttcacgttaa tcaggactcc tccctgcagg acggcgtatt
     1681 catctacaag gtgaagctgc tcggcaccaa cttccccccc gacggccccg taatgcagaa
     1741 gaagaccatg ggctgggagg cttcccccgg catctgggac ttcgtgaaga gcgccggcag
     1801 gaagatcggc atcggcagcg aggaggccgc ccccgccgcc gacgacctga agaaggagct
     1861 ggacagctac aagctgggca ccgacgacgt gaaggtggag gtggtgggcg acaaggccgt
     1921 gatcaccggc aacgtggccg accagagcat cctggagaag gccatcgtgg ccgtgggcaa
     1981 caccctgggc atcagcaagg tggagagcaa cgtggaggtg cccgacgcca aggagcccgt
     2041 gttccacacc gtgaagaagg gcgacaccct gtgggccatc gccagcaagc agtacggcaa
     2101 cgtcagcaag tacaacgcca tcttcgaggc caacaagccc atgctgagcc accccgacaa
     2161 gatctacccc ggccaggtgc tgaggatccc cgacctgggc tctgccccgg tttccgagtg
     2221 gatgtacccc gaggacggcg ccctgaagag cgagatcagg aaggggctga ggctgaagga
     2281 cggcggccac tacgccgccg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     2341 gcccggcgcc tacatcgtcg acatcgagtt ggacatcttg tcccacaacg tggactacac
     2401 catcgtggaa cagtgcgaac gcgccgtggg ccgccacccc accggtggca cggacgcgct
     2461 gtacaagggc gtcgacgaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca
     2521 ggacacgcag gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc
     2581 agccatcctg gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg
     2641 gcagaagaag ccacgttagg cggccgctcg agatcagcct cgactgtgcc ttctagttgc
     2701 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2761 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2821 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2881 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag tggcggtaat
     2941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3781 gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc
     3841 acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg
     3901 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
     3961 aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc
     4021 ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc
     4081 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     4141 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     4201 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4261 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4321 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4381 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4441 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
     4501 ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4561 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4621 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4681 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     4741 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     4801 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc
     4861 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     4921 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     4981 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     5041 cctcatcctg tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca
     5101 ctacttctgg aatagctcag aggccgggcc tcggcctctg cataaataaa aaaaattagt
     5161 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     5221 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5281 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     5341 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
     5401 tggttctttc cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag
     5461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//

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