pCAGGS-RSV-L(A2)

LOCUS      pCAGGS-RSV-L(A2)      11329 bp DNA     circular SYN 10-AUG-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..385
                     /label=CMV
                     /note="/vntifkey=30"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..737
                     /label=Chicken beta-actin promoter
                     /label=Chicken\beta-actin\promoter
                     /note="/vntifkey=29"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          738..1629
                     /label=intron
                     /note="/vntifkey=15"
     primer_bind     1343..1362
                     /label=pCAGGS-L-opt-test-F
     primer_bind     complement(1719..1740)
                     /label=pCAGGS-Vec-R
     primer_bind     1724..1761
                     /label=L-opt-F(pCAGGS)
     misc_feature    1741..1749
                     /label=Kozak sequence consensus
     primer_bind     1742..1761
                     /label=结合1
     CDS             1750..8247
                     /codon_start=1
                     /label=RSV-A2-L(opt)
                     /translation="MDPIINGNSANVYLTDSYLKGVISFSECNALGSYIFNGPYLKNDY
                     TNLISRQNPLIEHMNLKKLNITQSLISKYHKGEIKLEEPTYFQSLLMTYKSMTSSEQIA
                     TTNLLKKIIRRAIEISDVKVYAILNKLGLKEKDKIKSNNGQDEDNSVITTIIKDDILSA
                     VKDNQSHLKADKNHSTKQKDTIKTTLLKKLMCSMQHPPSWLIHWFNLYTKLNNILTQYR
                     SNEVKNHGFTLIDNQTLSGFQFILNQYGCIVYHKELKRITVTTYNQFLTWKDISLSRLN
                     VCLITWISNCLNTLNKSLGLRCGFNNVILTQLFLYGDCILKLFHNEGFYIIKEVEGFIM
                     SLILNITEEDQFRKRFYNSMLNNITDAANKAQKNLLSRVCHTLLDKTVSDNIINGRWII
                     LLSKFLKLIKLAGDNNLNNLSELYFLFRIFGHPMVDERQAMDAVKINCNETKFYLLSSL
                     SMLRGAFIYRIIKGFVNNYNRWPTLRNAIVLPLRWLTYYKLNTYPSLLELTERDLIVLS
                     GLRFYREFRLPKKVDLEMIINDKAISPPKNLIWTSFPRNYMPSHIQNYIEHEKLKFSES
                     DKSRRVLEYYLRDNKFNECDLYNCVVNQSYLNNPNHVVSLTGKERELSVGRMFAMQPGM
                     FRQVQILAEKMIAENILQFFPESLTRYGDLELQKILELKAGISNKSNRYNDNYNNYISK
                     CSIITDLSKFNQAFRYETSCICSDVLDELHGVQSLFSWLHLTIPHVTIICTYRHAPPYI
                     GDHIVDLNNVDEQSGLYRYHMGGIEGWCQKLWTIEAISLLDLISLKGKFSITALINGDN
                     QSIDISKPIRLMEGQTHAQADYLLALNSLKLLYKEYAGIGHKLKGTETYISRDMQFMSK
                     TIQHNGVYYPASIKKVLRVGPWINTILDDFKVSLESIGSLTQELEYRGESLLCSLIFRN
                     VWLYNQIALQLKNHALCNNKLYLDILKVLKHLKTFFNLDNIDTALTLYMNLPMLFGGGD
                     PNLLYRSFYRRTPDFLTEAIVHSVFILSYYTNHDLKDKLQDLSDDRLNKFLTCIITFDK
                     NPNAEFVTLMRDPQALGSERQAKITSEINRLAVTEVLSTAPNKIFSKSAQHYTTTEIDL
                     NDIMQNIEPTYPHGLRVVYESLPFYKAEKIVNLISGTKSITNILEKTSAIDLTDIDRAT
                     EMMRKNITLLIRILPLDCNRDKREILSMENLSITELSKYVRERSWSLSNIVGVTSPSIM
                     YTMDIKYTTSTISSGIIIEKYNVNSLTRGERGPTKPWVGSSTQEKKTMPVYNRQVLTKK
                     QRDQIDLLAKLDWVYASIDNKDEFMEELSIGTLGLTYEKAKKLFPQYLSVNYLHRLTVS
                     SRPCEFPASIPAYRTTNYHFDTSPINRILTEKYGDEDIDIVFQNCISFGLSLMSVVEQF
                     TNVCPNRIILIPKLNEIHLMKPPIFTGDVDIHKLKQVIQKQHMFLPDKISLTQYVELFL
                     SNKTLKSGSHVNSNLILAHKISDYFHNTYILSTNLAGHWILIIQLMKDSKGIFEKDWGE
                     GYITDHMFINLKVFFNAYKTYLLCFHKGYGKAKLECDMNTSDLLCVLELIDSSYWKSMS
                     KVFLEQKVIKYILSQDASLHRVKGCHSFKLWFLKRLNVAEFTVCPWVVNIDYHPTHMKA
                     ILTYIDLVRMGLINIDRIHIKNKHKFNDEFYTSNLFYINYNFSDNTHLLTKHIRIANSE
                     LENNYNKLYHPTPETLENILANPIKSNDKKTLNDYCIGKNVDSIMLPLLSNKKLIKSSA
                     MIRTNYSKQDLYNLFPMVVIDRIIDHSGNTAKSNQLYTTTSHQISLVHNSTSLYCMLPW
                     HHINRFNFVFSSTGCKISIEYILKDLKIKDPNCIAFIGEGAGNLLLRTVVELHPDIRYI
                     YRSLKDCNDHSLPIEFLRLYNGHINIDYGENLTIPATDATNNIHWSYLHIKFAEPISLF
                     VCDAELSVTVNWSKIIIEWSKHVRKCKYCSSVNKCMLIVKYHAQDDIDFKLDNITILKT
                     YVCLGSKLKGSEVYLVLTIGPANIFPVFNVVQNAKLILSRTKNFIMPKKADKESIDANI
                     KSLIPFLCYPITKKGINTALSKLKSVVSGDILSYSIAGRNEVFSNKLINHKHMNILKWF
                     NHVLNFRSTELNYNHLYMVESTYPYLSELLNSLTTNELKKLIKITGSLLYNFHNE"
     primer_bind     complement(2818..2841)
                     /label=pCAGGS-L-opt-test-R
     primer_bind     complement(8223..8247)
                     /label=L-opt-R(pCAGGS)
     primer_bind     8234..8268
                     /label=pCAGGS-Vec-F
     primer_bind     8248..8268
                     /label=结合2
     polyA_signal    8269..8809
                     /label=Rabbit beta-globin poly-A
                     /label=Rabbit\beta-globin\poly-A
                     /note="/vntifkey=25"
     polyA_signal    8416..8471
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8832..8848)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8856..8872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8880..8910)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8925..8946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    9000..9336
                     /label=SV40
                     /note="/vntifkey=21"
     promoter        9005..9200
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9051..9186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9206..9340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(9579..10167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10338..11198)
                     /codon_start=1
                     /label=Amp
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11199..11303)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcggccgc
     1741 gccgccacca tggaccccat catcaacggc aacagcgcca acgtgtacct gaccgacagc
     1801 tacctgaagg gcgtgatcag ctttagtgag tgcaacgccc tgggcagcta catcttcaac
     1861 ggcccctacc tgaagaacga ctacaccaac ctgatcagcc ggcagaaccc cctgatcgag
     1921 cacatgaacc tgaagaagct gaacatcacc cagagcctga tcagcaagta ccacaagggc
     1981 gagatcaagc tggaagaacc cacctacttc cagagcctgc tgatgaccta caagagcatg
     2041 accagcagcg agcagatcgc caccaccaac ctgctgaaga agatcatcag acgggccatc
     2101 gagatcagcg acgtgaaggt gtacgccatc ctgaacaagc tgggcctgaa agagaaggac
     2161 aagatcaaga gcaacaacgg ccaggacgag gacaacagcg tgatcaccac catcatcaag
     2221 gacgacatcc tgagcgccgt gaaggacaac cagagccacc tgaaggccga caagaaccac
     2281 agcaccaagc agaaggacac catcaagacc accctgctga aaaagctgat gtgcagcatg
     2341 cagcaccccc ccagctggct gatccactgg ttcaacctgt acaccaagct gaacaacatc
     2401 ctgacccagt accggtccaa cgaagtgaag aaccacggct tcaccctgat cgacaaccag
     2461 accctgagcg gcttccagtt catcctgaat cagtacggct gcatcgtgta ccacaaagag
     2521 ctgaagcgga tcaccgtgac cacctacaac cagtttctga cctggaagga catcagcctg
     2581 agccggctga acgtgtgcct gatcacctgg atcagcaact gcctgaacac cctgaacaag
     2641 agcctgggac tgagatgcgg cttcaacaac gtgatcctga ctcagctgtt cctgtacggc
     2701 gactgcatcc tgaagctgtt ccacaacgag ggcttctaca tcatcaaaga ggtggaaggc
     2761 ttcatcatga gcctgatcct gaatatcacc gaagaggacc agttccggaa gcggttctac
     2821 aacagcatgc tgaacaatat cacagacgcc gccaacaagg cccagaagaa cctgctgtcc
     2881 agagtgtgcc ataccctgct ggacaagacc gtgtccgaca acatcatcaa tggccggtgg
     2941 attatcctgc tgtctaagtt cctgaaactg attaagctgg ccggcgacaa caacctgaac
     3001 aatctgagcg agctgtactt cctgttccgg atcttcggcc accccatggt ggacgagaga
     3061 caggccatgg acgccgtgaa gatcaactgc aacgagacaa agttctatct gctgagttcc
     3121 ctgagcatgc tgagaggcgc cttcatctac cggatcatca agggcttcgt gaacaactac
     3181 aaccggtggc ccaccctgcg gaacgccatc gtgctgcccc tgcggtggct gacctactac
     3241 aagctgaata cctaccccag cctgctggaa ctgaccgagc gggacctgat cgtgctgagc
     3301 ggcctgagat tctacagaga gttccggctg cccaagaaag tggacctgga aatgatcatc
     3361 aacgacaagg ccatcagccc ccccaagaac ctgatctgga ccagcttccc ccggaactac
     3421 atgcccagcc acatccagaa ctacatcgag cacgagaagc tgaagttcag cgagagcgac
     3481 aagagcagac gggtgctgga atattacctg cgggacaaca agttcaacga gtgcgacctg
     3541 tacaactgcg tggtgaacca gtcctacctg aacaacccca accacgtggt gtccctgacc
     3601 ggcaaagaac gcgagctgag cgtgggccgg atgttcgcta tgcagcctgg catgttcaga
     3661 caggtgcaga tcctggccga gaagatgatc gccgagaaca tcctgcagtt cttcccagag
     3721 agcctgacca gatacggcga cctggaactg cagaagatcc tggaactgaa ggctggcatc
     3781 agcaacaaaa gcaaccggta caacgacaat tacaacaact acatcagcaa gtgcagcatc
     3841 atcaccgacc tgagcaagtt caaccaggcc ttcagatacg agacaagctg catctgcagc
     3901 gacgtgctgg acgagctgca tggcgtgcag agcctgttct cctggctgca cctgaccatc
     3961 ccccacgtga ccatcatctg cacctaccgg cacgcccctc cctacatcgg cgaccacatc
     4021 gtggatctga acaacgtgga cgagcagagc ggcctgtaca gataccacat gggcggcatc
     4081 gagggctggt gccagaagct gtggaccatc gaggccatct ccctgctgga tctgatcagc
     4141 ctgaagggca agttctccat caccgccctg atcaacggcg acaaccagtc catcgacatc
     4201 tccaagccca tccggctgat ggaaggccag acccacgccc aggccgacta cctgctggcc
     4261 ctgaatagcc tgaagctgct gtacaaagag tacgccggca tcggccacaa gctgaagggc
     4321 accgaaacct acatcagccg ggacatgcag ttcatgagca agaccatcca gcacaacggc
     4381 gtgtactacc ccgccagcat caagaaagtg ctgagagtgg gcccctggat caacaccatc
     4441 ctggacgact tcaaggtgtc cctggaatcc atcggcagcc tgacccagga actggaatac
     4501 cggggcgagt ccctgctgtg cagcctgatc ttccggaacg tgtggctgta caaccagatc
     4561 gccctgcagc tgaagaatca cgccctgtgc aacaacaagc tgtacctgga catcctgaag
     4621 gtgctgaagc acctgaaaac ctttttcaac ctggacaaca tcgataccgc cctgaccctg
     4681 tacatgaatc tgcccatgct gttcggcgga ggcgacccca atctgctgta cagaagcttc
     4741 taccggcgga cccccgactt cctgaccgag gccatcgtgc acagcgtgtt catcctgtcc
     4801 tactacacca atcacgacct gaaggataag ctgcaggacc tgagcgacga ccggctgaac
     4861 aagtttctga catgtatcat caccttcgac aagaatccca acgccgagtt cgtgaccctg
     4921 atgcgggatc ctcaggctct gggctctgag cggcaggcca agatcaccag cgagatcaac
     4981 cggctggccg tgaccgaggt gctgtccacc gcccccaaca agatcttcag caagagcgcc
     5041 cagcactaca ccaccaccga gatcgacctg aacgacatca tgcagaacat cgagcccacc
     5101 tacccccacg gcctgagagt ggtgtatgag agcctgccct tctacaaggc cgagaaaatc
     5161 gtgaatctga tctccggcac caagagcatc accaacatcc tggaaaagac cagcgccatc
     5221 gacctgaccg atatcgaccg ggccaccgag atgatgcgga agaacatcac actgctgatc
     5281 cggatcctgc ccctggactg caaccgggac aagcgcgaga tcctgagcat ggaaaacctg
     5341 agcatcaccg agctgtccaa atacgtgcgc gagcggagct ggtccctgag caacatcgtg
     5401 ggcgtgacca gccccagcat catgtacacc atggacatca agtacaccac cagcaccatc
     5461 agcagcggca tcatcatcga gaagtacaac gtgaactccc tgaccagagg cgagagaggc
     5521 cccaccaagc cctgggtggg aagcagcacc caggaaaaga aaaccatgcc cgtgtacaat
     5581 cgccaggtgc tgaccaagaa gcagcgggac cagattgatc tgctggccaa gctggactgg
     5641 gtgtacgcct ccatcgacaa caaggacgag ttcatggaag aactgtctat cggcaccctg
     5701 ggcctgacct acgagaaggc caagaagctg tttccccagt acctgagcgt gaactacctg
     5761 caccggctga ccgtgtccag ccggccttgc gagttccctg ccagcatccc cgcctaccgg
     5821 accaccaact accacttcga caccagcccc atcaaccgga tcctgacaga gaagtacggc
     5881 gacgaggaca tcgacatcgt gttccagaac tgcatcagct tcggcctgag cctgatgagc
     5941 gtggtggaac agttcaccaa cgtgtgcccc aacagaatca tcctgatccc caagctgaat
     6001 gagatccacc tgatgaagcc ccccatcttc accggggacg tggacatcca caaactgaaa
     6061 caggtgatcc agaaacagca catgtttctg cccgacaaga tctccctgac acagtacgtg
     6121 gaactgttcc tgtccaacaa gaccctgaag tccggcagcc acgtgaacag caacctgatc
     6181 ctggcccaca agatcagcga ttacttccac aacacctaca tcctgtccac caatctggct
     6241 ggacactgga tcctgatcat tcagctgatg aaggacagca agggcatctt cgagaaggac
     6301 tggggcgagg gctacatcac cgatcacatg ttcatcaacc tgaaggtgtt cttcaacgcc
     6361 tacaagacat acctgctgtg cttccacaag ggctacggca aggccaaact ggaatgcgac
     6421 atgaacacca gcgatctgct gtgcgtgctg gaactgatcg acagcagcta ctggaagtct
     6481 atgagcaaag tgttcctgga acagaaagtg atcaagtata tcctgagcca ggacgccagc
     6541 ctgcacagag tgaagggctg ccactccttc aagctgtggt tcctgaagag actgaacgtg
     6601 gccgagttca ccgtgtgccc ttgggtggtg aacatcgact accaccccac ccacatgaag
     6661 gccatcctga cctacatcga cctggtgcgc atgggcctga tcaacatcga ccggatccac
     6721 atcaagaaca agcacaagtt caatgacgag ttctacacca gcaacctgtt ctacatcaac
     6781 tacaacttca gcgacaacac ccatctgctg acaaagcaca tccggatcgc caacagcgag
     6841 ctggaaaaca actataacaa actgtaccac cctacccccg agacactgga aaacatcctg
     6901 gccaacccca tcaagtccaa cgacaagaaa accctgaacg actactgcat cggcaagaac
     6961 gtggacagca tcatgctgcc tctgctgagc aacaagaagc tgatcaagtc cagcgccatg
     7021 atccggacca actacagcaa gcaggatctg tacaacctgt tccctatggt ggtgatcgac
     7081 aggatcatcg accacagcgg caataccgcc aagtccaacc agctgtacac cacaaccagc
     7141 caccagatca gcctggtgca caacagcacc agcctgtact gcatgctgcc ctggcaccac
     7201 atcaaccggt tcaacttcgt gttcagcagc accggctgca agatcagcat cgagtacatt
     7261 ctgaaggacc tgaagatcaa ggaccccaac tgtatcgcct tcatcggcga gggcgctggc
     7321 aacctgctgc tgcggacagt ggtggaactg caccccgaca tccggtacat ctacagaagc
     7381 ctgaaggact gcaacgacca cagcctgcct atcgagttcc tgagactgta caacggccac
     7441 atcaatatcg actacggcga gaacctgaca atccccgcca ccgacgccac caacaacatc
     7501 cactggtctt acctgcacat caagttcgcc gagcccatca gcctgttcgt gtgcgacgcc
     7561 gagctgtctg tgaccgtgaa ctggtccaag atcatcattg agtggtccaa gcacgtgcgg
     7621 aagtgcaagt actgcagcag cgtgaacaag tgcatgctga tcgtgaagta ccatgcccag
     7681 gacgatatcg acttcaagct ggataacatc accatcctga aaacatacgt gtgtctgggc
     7741 agcaagctga aaggcagcga ggtgtacctg gtgctgacaa tcggccctgc caacatcttc
     7801 cccgtgttca acgtggtgca gaacgccaag ctgatcctgt cccggaccaa gaacttcatc
     7861 atgcccaaga aggctgacaa agagagcatc gacgccaata tcaagtccct gatccccttc
     7921 ctgtgctacc ccatcaccaa gaagggcatc aacaccgccc tgtccaagct gaagtccgtg
     7981 gtgtccggcg acatcctgtc ttactctatc gccggcagaa acgaggtgtt cagcaacaaa
     8041 ctgatcaacc acaagcacat gaatatcctg aagtggttca accacgtgct gaacttccgg
     8101 tccaccgagc tgaactacaa tcatctgtac atggtggaaa gcacctaccc ctacctgtcc
     8161 gagctgctga acagcctgac caccaatgag ctgaagaaac tgatcaagat caccggcagc
     8221 ctgctgtata acttccacaa tgagtgatag gtaccatcga taccggtgaa ttcactcctc
     8281 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     8341 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     8401 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     8461 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     8521 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     8581 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     8641 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     8701 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     8761 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     8821 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8881 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8941 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     9001 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9121 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9181 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     9241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9301 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     9361 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//