| 质粒名称: | pYK64 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000787 |
| 产品用途: | Mammalian PhCMV-driven constitutive SEAP expression vector containing 24 tandem MS2-box repeats in the 3’UTR (PhCMV-SEAP-(MS2-box)24-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP-(MS2-box)24 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK64
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK64载体质粒基本信息
pYK64质粒图谱载体图谱和pYK64载体序列质粒序列多克隆位点信息
pYK64质粒载体简介
pYK64质粒序列载体序列
LOCUS pYK64 8235 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8235)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8235
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..917
/label=oYK55-SEAP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 900..927
/label=oDJ273-1
misc_feature 901..2460
/label=SEAP
primer_bind complement(2439..2460)
/label=oDJ273-2
primer_bind complement(2441..2475)
/label=pYK62-SEAP-R
primer_bind complement(2441..2460)
/label=oYK55-SEAP-R
primer_bind complement(2444..2460)
/label=Oscl31-4
primer_bind complement(2444..2460)
/label=oSCL083
polyA_signal 2452..2460
/label=SV40 late polyA
misc_feature 2469..2473
/label=New Feature
misc_feature 2469..2473
/label=New Feature
misc_feature 2492..2495
/label=linker
primer_bind complement(2497..2511)
/label=Oscl28-2
primer_bind complement(2498..2511)
/label=8x R
misc_feature 2512..2524
/label=linker
misc_feature 2530..2548
/label=MS2 box
misc_feature 2554..2563
/label=linker
misc_feature 2569..2587
/label=MS2 box
primer_bind complement(2580..2600)
/label=oYK55-SEAP-R
misc_feature 2593..2602
/label=linker
misc_feature 2593..2602
/label=linker
misc_feature 2601..2631
/label=hairpin
misc_feature 2607..2625
/label=MS2 box
misc_feature 2630..2642
/label=linker
misc_feature 2641..2671
/label=hairpin
misc_feature 2647..2665
/label=MS2 box
misc_feature 2675..2680
/label=linker
misc_feature 2689..2704
/label=linker
misc_feature 2710..2713
/label=linker
primer_bind complement(2715..2729)
/label=Oscl28-2
primer_bind complement(2716..2729)
/label=8x R
misc_feature 2730..2742
/label=linker
misc_feature 2748..2766
/label=MS2 box
misc_feature 2772..2781
/label=linker
misc_feature 2787..2805
/label=MS2 box
primer_bind complement(2798..2818)
/label=oYK55-SEAP-R
misc_feature 2811..2820
/label=linker
misc_feature 2811..2820
/label=linker
misc_feature 2819..2849
/label=hairpin
misc_feature 2825..2843
/label=MS2 box
misc_feature 2848..2860
/label=linker
misc_feature 2859..2889
/label=hairpin
misc_feature 2865..2883
/label=MS2 box
misc_feature 2893..2898
/label=linker
misc_feature 2907..2922
/label=linker
misc_feature 2928..2931
/label=linker
primer_bind complement(2933..2947)
/label=Oscl28-2
primer_bind complement(2934..2947)
/label=8x R
misc_feature 2948..2960
/label=linker
misc_feature 2966..2984
/label=MS2 box
misc_feature 2990..2999
/label=linker
misc_feature 3005..3023
/label=MS2 box
primer_bind complement(3016..3036)
/label=oYK55-SEAP-R
misc_feature 3029..3038
/label=linker
misc_feature 3029..3038
/label=linker
misc_feature 3037..3067
/label=hairpin
misc_feature 3043..3061
/label=MS2 box
misc_feature 3066..3078
/label=linker
misc_feature 3077..3107
/label=hairpin
misc_feature 3083..3101
/label=MS2 box
misc_feature 3111..3116
/label=linker
misc_feature 3125..3140
/label=linker
misc_feature 3167..3179
/label=linker
misc_feature 3185..3203
/label=MS2 box
misc_feature 3209..3218
/label=linker
misc_feature 3224..3242
/label=MS2 box
primer_bind complement(3235..3255)
/label=oYK55-SEAP-R
misc_feature 3248..3257
/label=linker
misc_feature 3248..3257
/label=linker
misc_feature 3256..3286
/label=hairpin
misc_feature 3262..3280
/label=MS2 box
misc_feature 3285..3297
/label=linker
misc_feature 3296..3326
/label=hairpin
misc_feature 3302..3320
/label=MS2 box
misc_feature 3330..3335
/label=linker
misc_feature 3344..3359
/label=linker
misc_feature 3365..3377
/label=linker
misc_feature 3383..3401
/label=MS2 box
misc_feature 3407..3416
/label=linker
misc_feature 3422..3440
/label=MS2 box
primer_bind complement(3433..3453)
/label=oYK55-SEAP-R
misc_feature 3446..3455
/label=linker
misc_feature 3446..3455
/label=linker
misc_feature 3454..3484
/label=hairpin
misc_feature 3460..3478
/label=MS2 box
misc_feature 3483..3495
/label=linker
misc_feature 3494..3524
/label=hairpin
misc_feature 3500..3518
/label=MS2 box
misc_feature 3528..3533
/label=linker
misc_feature 3542..3557
/label=linker
misc_feature 3563..3575
/label=linker
misc_feature 3581..3599
/label=MS2 box
misc_feature 3605..3614
/label=linker
misc_feature 3620..3638
/label=MS2 box
primer_bind complement(3631..3651)
/label=oYK55-SEAP-R
misc_feature 3644..3653
/label=linker
misc_feature 3644..3653
/label=linker
misc_feature 3652..3682
/label=hairpin
misc_feature 3658..3676
/label=MS2 box
misc_feature 3681..3693
/label=linker
misc_feature 3692..3722
/label=hairpin
misc_feature 3698..3716
/label=MS2 box
misc_feature 3726..3731
/label=linker
misc_feature 3740..3755
/label=linker
terminator 3829..4056
/label=bGH PA
misc_feature 4102..4530
/label=f1 origin
misc_feature 4535..4878
/label=SV40 early promoter and origin
misc_feature 4724..4801
/label=SV40 ORI
CDS 4943..5734
/label=Kan/neoR
misc_feature 5908..6038
/label=SV40 early polyadenylation signal
primer_bind complement(6074..6094)
/label=M13-rev
misc_binding complement(6100..6122)
/label=LacO
promoter complement(6127..6156)
/label=lac
misc_feature complement(6421..7094)
/label=pUC origin
CDS complement(7242..7901)
/label=AmpR
promoter complement(8141..8169)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgctgctgc tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca
961 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
1021 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
1081 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
1141 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
1201 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
1261 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
1321 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
1381 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
1441 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
1501 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
1561 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
1621 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
1681 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
1741 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
1801 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
1861 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
1921 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
1981 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
2041 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
2101 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
2161 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
2221 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
2281 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
2341 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
2401 gacgccgcgc acccgggtta ctctagagtc ggggcggccg gccgcttcga gcagacatga
2461 aaaccccaag cttggtaccg agctcggatc caacttgaac cggtaagaat tgcaacaaac
2521 aaatcaccac cgtgaggatc acccacggtg gtgaaagcct tagcctagcc gtgaggatca
2581 cccacggtag gcacaagaaa ttctacccgt gaggatcacc cacgggtaga atcacaaaga
2641 ctacagccgt gaggatcacc cacggctgta gactcaaact taaaaaggat atcttcagca
2701 aactgatcca acttgaaccg gtaagaattg caacaaacaa atcaccaccg tgaggatcac
2761 ccacggtggt gaaagcctta gcctagccgt gaggatcacc cacggtaggc acaagaaatt
2821 ctacccgtga ggatcaccca cgggtagaat cacaaagact acagccgtga ggatcaccca
2881 cggctgtaga ctcaaactta aaaaggatat cttcagcaaa ctgatccaac ttgaaccggt
2941 aagaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
3001 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
3061 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
3121 aaggatatct tcagcaaact gatcggagac gagtccgtct caaattgcaa caaacaaatc
3181 accaccgtga ggatcaccca cggtggtgaa agccttagcc tagccgtgag gatcacccac
3241 ggtaggcaca agaaattcta cccgtgagga tcacccacgg gtagaatcac aaagactaca
3301 gccgtgagga tcacccacgg ctgtagactc aaacttaaaa aggatatctt cagcaaactg
3361 aattgcaaca aacaaatcac caccgtgagg atcacccacg gtggtgaaag ccttagccta
3421 gccgtgagga tcacccacgg taggcacaag aaattctacc cgtgaggatc acccacgggt
3481 agaatcacaa agactacagc cgtgaggatc acccacggct gtagactcaa acttaaaaag
3541 gatatcttca gcaaactgaa ttgcaacaaa caaatcacca ccgtgaggat cacccacggt
3601 ggtgaaagcc ttagcctagc cgtgaggatc acccacggta ggcacaagaa attctacccg
3661 tgaggatcac ccacgggtag aatcacaaag actacagccg tgaggatcac ccacggctgt
3721 agactcaaac ttaaaaagga tatcttcagc aaactgaatt ctgcagatat ccagcacagt
3781 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
3841 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
3901 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
3961 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
4021 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
4081 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
4141 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
4201 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
4261 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
4321 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
4381 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
4441 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
4501 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
4561 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
4621 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
4681 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
4741 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
4801 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
4861 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
4921 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
4981 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
5041 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
5101 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
5161 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
5221 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
5281 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
5341 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
5401 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
5461 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
5521 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
5581 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
5641 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
5701 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
5761 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
5821 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
5881 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
5941 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
6001 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
6061 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
6121 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
6181 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
6241 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
6301 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
6361 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
6421 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
6481 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
6541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
6601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
6661 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
6781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6901 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
6961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
7021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
7081 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
7141 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
7201 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
7261 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
7321 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
7381 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
7441 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
7501 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
7561 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
7621 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
7681 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
7741 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
7801 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
7861 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
7921 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
7981 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
8041 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
8101 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
8161 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
8221 gtgccacctg acgtc
//
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