Tet-O-FUW-Brn2

LOCUS       Tet-O-FUW-Brn2                9746 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Tet-O-FUW-Brn2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Direct conversion of fibroblasts to functional neurons by defined 
            factors.
  JOURNAL   Nature. 2010 Feb 25. 463(7284):1035-41.
  PUBMED    20107439
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|Tet-O-FUW-Brn2|
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      10..19
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    1393..1981
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1446..1466)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1864..1875)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1984..2000)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1985..2001)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2506..2686
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2712..2729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2718..2942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2988..3416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3075..3094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3285..3306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3425..3445)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3430..3759
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3610..3745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3672..3691
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3807..3854
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3873..4247
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4377..4498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4414..4433)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4468..4487
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4547..4563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4547..4563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4560..4582)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4571..4587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4595..4625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4640..4661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4778..4795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4949..5537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5029..5048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5708..6568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6331..6350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6569..6673)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6748..6767)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6939..7318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7320..7518
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7473..7493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7536..7716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7763..7888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8381..8614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9141..9258
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9312..9582
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9615..9652
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9649..9673
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattcgctag ccaccatggc gaccgcagcg tctaaccact acagcctgct cacctccagc
       61 gcctccatcg tacatgccga gccgcctggc ggcatgcagc agggcgcagg gggctaccgc
      121 gaggcgcaga gcctggtgca gggcgactac ggcgcgctgc agagcaacgg gcacccgctc
      181 agccacgctc accagtggat caccgcgctg tcccacggcg gcggcggcgg gggcggcggc
      241 ggcggtggag gaggcggggg aggcggcggg ggaggcggcg acggctcccc gtggtccacc
      301 agccccctag gccagccgga catcaagccc tcggtggtgg tacagcaggg tggccgaggc
      361 gacgagctgc acgggccagg agcgctgcag caacagcatc aacagcaaca gcaacagcag
      421 cagcagcagc agcagcagca gcagcagcaa cagcagcagc aacaacagcg accgccacat
      481 ctggtgcacc acgctgccaa ccaccatccc gggcccgggg catggcggag tgcggcggct
      541 gcagctcacc tccctccctc catgggagct tccaacggcg gtttgctcta ttcgcagccg
      601 agcttcacgg tgaacggcat gctgggcgca ggagggcagc cggctgggct gcaccaccac
      661 ggcctgaggg acgcccacga tgagccacac catgcagacc accacccgca tccgcactct
      721 cacccacacc agcaaccgcc cccgccacct cccccacaag gcccaccggg ccacccaggc
      781 gcgcaccacg acccgcactc ggacgaggac acgccgacct cagacgacct ggagcagttc
      841 gccaagcaat tcaagcagag gcggatcaaa ctcggattta ctcaagcaga cgtggggctg
      901 gcgcttggca ccctgtacgg caacgtgttc tcgcagacca ccatctgcag gtttgaggcc
      961 ctgcagctga gcttcaagaa catgtgcaag ctgaagcctt tgttgaacaa gtggttggaa
     1021 gaggcagact catcctcggg cagccccacc agcatagaca agatcgcagc gcaagggcgc
     1081 aaacggaaaa agcggacctc catcgaggtg agcgtcaagg gggctctgga gagccatttc
     1141 ctcaaatgcc ctaagccctc ggcccaggag atcacctccc tcgcggacag cttacagctg
     1201 gagaaggagg tggtgagagt ttggttttgt aacaggagac agaaagagaa aaggatgacc
     1261 cctcccggag ggactctgcc gggcgccgag gatgtgtatg ggggtagtag ggacacgcca
     1321 ccacaccacg gggtgcagac gcccgtccag tgagctagga ataagggcga attcgatatc
     1381 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     1441 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     1501 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     1561 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     1621 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     1681 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     1741 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     1801 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     1861 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     1921 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     1981 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     2041 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     2101 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     2161 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2221 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     2281 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     2341 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     2401 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     2461 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     2521 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2581 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2641 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     2701 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2761 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2821 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2881 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2941 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3421 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3481 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3541 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3601 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3661 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     3721 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     3781 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     3841 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     3901 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     3961 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     4021 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     4081 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     4141 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     4201 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     4261 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4321 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     4381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4441 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4501 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4561 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4621 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4681 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4741 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5521 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5641 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5701 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5761 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5821 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5881 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5941 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6001 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6061 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6121 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6181 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6241 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6301 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6361 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6421 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6481 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6541 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6601 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6661 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     6721 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6781 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     6841 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     6901 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     6961 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     7021 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     7081 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     7141 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     7201 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     7261 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     7321 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     7381 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     7441 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     7501 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     7561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7621 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7681 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7741 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7801 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7861 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7921 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7981 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     8041 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     8101 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8161 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8221 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8281 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8341 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8401 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8461 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8521 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8581 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8641 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8701 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8761 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8821 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8881 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8941 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     9001 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     9061 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     9121 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9181 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9241 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttactc
     9301 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     9361 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     9421 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     9481 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     9541 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     9601 ggtacccggg tcgagtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
     9661 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     9721 ggaccgatcc agcctccgcg gccccg
//