| 质粒名称: | Lenti-Hb9-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 37080 |
| 存储实验室: | Fred Gage |
|
载体骨架基本信息 |
|
| 载体名称: | CSCspPW |
| 载体出品公司: | Inder Verma lab |
| 空载体大小: | 8466 bp |
| 完整质粒大小: | 12486 bp |
| 载体类型: | 慢病毒载体 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Hb9 GFP |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 4020 bp |
| 启动子: | Hb9 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | ClaI(未破坏) |
| 3’克隆位点: | XbaI(未破坏) |
| 5’测序引物 | GCTAGCTCCGGAGAGCAAAAGCTG |
| 3’测序引物 | TGTAATCCAGAGGTTGATTG |
|
文献引用方法 |
|
| 材料和方法部分: | Lenti-Hb9-GFP was a gift from Fred Gage (Addgene plasmid # 37080 ; http://n2t.net/addgene:37080 ; RRID:Addgene_37080) |
| 参考文献部分: | Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor neurons derived from human embryonic stem cells. Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH. Cell Stem Cell. 2008 Dec 4;3(6):649-57. 10.1016/j.stem.2008.10.001 PubMed 19041781 |
买家导航
Lenti-Hb9-GFP载体质粒基本信息
Lenti-Hb9-GFP质粒图谱载体图谱和Lenti-Hb9-GFP载体序列质粒序列多克隆位点信息
Lenti-Hb9-GFP质粒载体简介
Lenti-Hb9-GFP质粒序列载体序列
LOCUS Lenti-Hb9-GFP 12313 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION 3rd generation lentiviral transfer plasmid for Hb9 GFP.
ACCESSION .
VERSION .
KEYWORDS Lenti-Hb9-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12313)
AUTHORS Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
TITLE Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor
neurons derived from human embryonic stem cells.
JOURNAL Cell Stem Cell. 2008 Dec 4;3(6):649-57.
PUBMED 19041781
REFERENCE 2 (bases 1 to 12313)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12313
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 616..814
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 832..1012
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1059..1184
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1677..1910
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2095..2139
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2391..2507
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6138..6176
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 6208..6927
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 6208..6924
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6253..6274)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6514..6533)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6861..6882
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 7120..7708
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7173..7193)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7591..7602)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8115..8295
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8321..8338)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8327..8551
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8597..9025
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8684..8703)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8894..8915
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9034..9054)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9039..9368
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9219..9354
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9281..9300
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9416..9463
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 9482..9856
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 9986..10107
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10023..10042)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10077..10096
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10156..10172)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10156..10172)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10169..10191)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10180..10196
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10204..10234)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10249..10270
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10387..10404)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10558..11146)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10638..10657)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11317..12177)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11940..11959
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12178..12282)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgcggccg
2521 ctcgactcga tcctgtctcc ctggccagga gtctggggtc cgcagatata ttacaacaaa
2581 attactaatt ggaaaatgaa attgtcaata aagcacaccc cacgcgtgta ctatatcttt
2641 aaatattatg atgtcttatt ggtcccattt ccttacaata atttattgca ataaaacaga
2701 cactgcaggt ccccttaaat tgtcttttca taatgaataa atttaagcag gctaattaat
2761 atataaacta gctcaatttg tcaagttgat ttgtatttta gttaattgtg aaagtaatta
2821 ccacatggtc aaattaacag ctttctggaa atgaccaagc ctgaggtttt atttccttcc
2881 tgggtgaaga aaattcattt ttccaagctc ttgatgtgat gaataaaagt cataaatctg
2941 ggtgattggt gcaggcagag tctaaatggc ttcatatttc attttaggtt taatagaaat
3001 attcatgctc tgttttaatg aaattaaatt gaagggggat ggggctctgg aggcggccct
3061 ggctgggggc ttaaagggac agtgcacttt tcctttcttc atgcaagggc cagggctaga
3121 ggtccctgga ctcagtcaag aagtggagag agctgaggga ggcctgggag gcccttattc
3181 tgtacccagt gtagcacacc tgcagccctg attggggggg ggggcggggg caattcacac
3241 cctgtattct caccaggcta aagtttcttc tatctggttc cttctctcca aaaaatcagt
3301 cagaagggac ctcccttggc tgagagtaga agtgaggtgg tcagcgtgct gcagacaagc
3361 ctgtcctcca tctgcctaag gggtctgata tcctacacca agccccccct accccttctg
3421 gagacacata ggagataagc attttcaaaa ccaccatggc actctcctgt tctcccctgc
3481 accccttcct cccagactcc atcctgcctg aggatgattt ggtccaactt gaataataga
3541 gtactctctt tccacatctt tccacatcgg aaagagtagt ccgggatcca gtccacctgc
3601 agccctgatt aaaggaagga aggaagacta gaggcgcagt tctacctatt atacttattt
3661 taaggttccc aggtcagaga tgcctcatgg acatttctcc ctaaggcagg tcctgaccag
3721 ccctccccct gaatggtgct gatctgtgac ttgttgcttc tattgaacaa atacagcttt
3781 gatttgtttc cagccagagt ggttagctga tgaattgaca aaaactaatc agctttattg
3841 ggaaacaggt ttaagggcac ggacgtgtca ataacgctca gcctgacccc ctcttccatt
3901 agctaggcag gctgattaga gtaagaagac cctctcgccg attgcaccga aattgctttc
3961 ataaactcac aatattagca gtcgatgtga gcagctaatt tagagaagac atccaggccc
4021 gtcacatcga tcatcgggtt tatgtatcat tatctcattt acagaatgtc gcagtgtaat
4081 acatgttttt ggcaataggc ttggccgctc gccggagccg cgcgctgttc ctttcggcca
4141 cactgcaggg gacttcctca acaatctacg atgtctcttc gcgtgaacaa ggaggcactt
4201 tgcacacata tgcacacttg cgcccaggct ctcagtgcgc gctcagtgca cacatgcacg
4261 tggacacatg ctccgacata ctacctgagc tgcctttcct tctcatgcgg ggatgctggc
4321 attgctctct cctgcgagcc cctggtctga agcccaatgc cggacgctca ccaaccttgg
4381 agtcaacctc ggagagctca aggttcggtt ctgtcaccag catacagaga tggtgggtgg
4441 cggcgcaatc agccaccttc tctcaaggct ttggtcgccc agttggcggg tgtccagaaa
4501 tccacaggcc tgcgaaggtc cttctccgcc ctcccaggct ccagcttgac ctgccctgcc
4561 ctgggcgggc ctttctcgca acacttccag gctcagccag atgcgcctgg tcttagggag
4621 tgggccacct gttcctttgg gtaattattt taatgtaagg ggtctacttc aggcctcctg
4681 cagtgacaga cggcagcgga gctgcaaagc gctctcagcc tttcagtgga atttcccaga
4741 tgggccaagg ggaggccagg gcgggtcacc aggggccctt taacgagttc attagccaaa
4801 ttatcttgcc tgcagcaagg accccagaga ctgtagggag agtaggggag aaggtgggtc
4861 tttctccctc cctgctagtc tgcaagaaca gttgtgggaa ggaaagagaa aggacatcct
4921 ccccaaaggg gcctaggaac actaaaggca aagatggcat ctctgatgtg acttatgcgc
4981 cagaccagcg aggtggtgga gtggtgggaa ggaatcataa tgattaggaa acacggagat
5041 tctaacaacc agggcactga cccatattgt ggaacaaacc tagtgcagaa atcaaagtca
5101 gagcccctgg aactttattc tgtgcttctt aagcaaagaa cctggacatc ttggagcctt
5161 ggtctgtccg tccatctgta gagtagaagg taggtgtgtt tccttccaca atttgtgaga
5221 tcactgccct aagaaaagca actctgggaa gagcttctgg catgaccctg ttgggtcctt
5281 cgggccccct tactcctgtg gtaggaggct ttggcaggtt gaggtgagcc tgatcctaga
5341 tgcctgtgct ggaaaatgaa cccaactctc acctgtcgtc gcgcccccca cccccacccc
5401 acagctgtga ggcgctgctc tggaggtagc tcagcatccc cgcccctcct ccttgtcctc
5461 actctgcctc ctgtagtgtt agcttctgct acctcagcaa gtagtgagtg aatagaccaa
5521 ttagccagga cttcctgcca ttatctgctg tagacttggg cattagtacg ccctgattca
5581 ccataatttc aagtcagaac acactaaaaa attcactcac cgaggcatta aaatgttccc
5641 agacaacgtc tgtgtttgtg taactgggtt tcttcccttc gtgtttgatg cacaccagca
5701 tcatataggt cagtggtgga gtccaggcta ggcagacaat ttttgtcgct ttttgtttgt
5761 ttttatattt ttaaaacatg aattgcattg tttcccggtg gttgtaaact ttgtttctgg
5821 ggcttcagta ttgccagtgt aatcttcttc tgcttgatca ggggcaaaca agtgacttag
5881 aaagttgtgg gagtgcccta agaacagtag ccagagcctt tacagagcca ggctttgtgt
5941 cctaaacgta catcgcccca gctatgggaa gctgggttat caccttcaaa gcagaccctt
6001 tctgtggatg acttaccaag ccctgcaggt gggtagtgga ggtggggaga gggctgcaca
6061 gcctgcttgg agcttgcttc tgttctttgt cctgcccatg atatgaagct cctatacaat
6121 gaatttgcag taagatcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
6181 tagtgaaccg cagatccggc gcgccccatg gtgagcaagg gcgaggagct gttcaccggg
6241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
6301 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
6361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
6421 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
6481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
6541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
6601 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
6661 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
6721 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
6781 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
6841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
6901 ctcggcatgg acgagctgta caagtaaagc ggccaattcg gatccgttta aactctagag
6961 gatccaccgg tggccgcctg ggcccgttaa cgtctcgagt ttaaacctgg aaaaacatgg
7021 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
7081 agaggaggag gaggtgggtt ttccagtcac acctcagaca atcaacctct ggattacaaa
7141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
7201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
7261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
7321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
7381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
7441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
7501 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
7561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
7621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
7681 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa
7741 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
7801 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
7861 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
7921 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
7981 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
8041 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
8101 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
8161 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
8221 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
8281 ggaaaatctc tagcagggcc gctttaaacc cgctgatcag cctcgactgt gccttctagt
8341 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
8401 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
8461 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
8521 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
8581 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
8641 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
8701 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
8761 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
8821 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
8881 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
8941 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
9001 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
9061 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
9121 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
9181 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
9241 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
9301 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
9361 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
9421 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
9481 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
9541 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
9601 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
9661 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
9721 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
9781 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
9841 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
9901 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
9961 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
10021 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
10081 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
10141 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
10201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
10261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
10321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
10381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
10441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
10501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
10561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
10621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
10681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
10741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
10801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
10861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
10921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
10981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
11041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
11101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
11161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
11221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
11281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
11341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
11401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
11461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
11521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
11581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
11641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
11701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
11761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
11821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
11881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
11941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
12001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
12061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
12121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
12181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
12241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
12301 gccacctgac gtc
//
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