pYK91

LOCUS       pYK91           7834 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..232
                     /label=oYK403_MluI_F
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..928
                     /label=oYK317_NLS_NS3a_F
     primer_bind     881..928
                     /label=oYK320_NES_F
     primer_bind     881..927
                     /label=oYK30-NS3a_Head-F
     primer_bind     881..925
                     /label=oYK407_Flag_NS3a_F
     primer_bind     881..909
                     /label=oYK36-AG-Link_s-F
     primer_bind     881..909
                     /label=oYK255_AG_NS3a
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     887..904
                     /label=oYK663_Nhe_F
     primer_bind     904..924
                     /label=oYK808_Ns3a_F
     CDS             910..1497
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     910..945
                     /label=oSCL1082
     primer_bind     910..930
                     /label=oyk03-03-F
     primer_bind     910..930
                     /label=oYK04-ns3a-F
     primer_bind     910..928
                     /label=oYK25-NS3a-F
     primer_bind     910..928
                     /label=oYK373_NS3a_F
     primer_bind     927..985
                     /label=oSCL1083
     primer_bind     966..1024
                     /label=oSCL1084
     primer_bind     1003..1061
                     /label=oSCL1085
     primer_bind     1040..1098
                     /label=oSCL1086
     primer_bind     complement(1068..1087)
                     /label=oSCL1106
     primer_bind     1069..1107
                     /label=oSCL1104
     primer_bind     complement(1304..1362)
                     /label=oSCL1105
     primer_bind     complement(1344..1415)
                     /label=oSCL1087
     primer_bind     1346..1367
                     /label=oSCL1107
     primer_bind     1397..1415
                     /label=oSCL1088
     primer_bind     complement(1479..1503)
                     /label=oSCL1089
     primer_bind     complement(1482..1514)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(1485..1526)
                     /label=oYK66-NS3a-R
     misc_feature    1504..1514
                     /label=linker
     CDS             1507..1581
                     /codon_start=1
                     /label=5xGS Linker
                     /translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
     primer_bind     1507..1527
                     
     primer_bind     1507..1525
                     /label=Primer 1
     primer_bind     complement(1556..1584)
                     /label=olyl212
     primer_bind     complement(1556..1581)
                     /label=olyl234
     primer_bind     complement(1565..1601)
                     /label=oYK45-46 ANR1-R
     primer_bind     1581..1603
                     /label=oYK255_AG_NS3a
     primer_bind     1581..1600
                     /label=oYK36-AG-Link_s-F
     primer_bind     1582..1601
                     /label=oYK41-44 AG-LplA-F
     primer_bind     1582..1601
                     /label=oYK45-46 AG-LplA-F
     misc_feature    1585..2259
                     /label=AG
     primer_bind     2033..2043
                     /label=oSCL1104
     misc_feature    2260..2310
                     /label=Short_Linker
     primer_bind     complement(2286..2330)
                     /label=oYK36-AG-Link_s-R
     primer_bind     complement(2286..2310)
                     /label=oYK33-35-PB1-AG-R
     misc_feature    2311..3332
                     /label=LPLA
                     /note="LplA"
     primer_bind     2311..2329
                     /label=oYK36-37-LplA-F
     primer_bind     2587..2609
                     /label=oYK08_lpl91-F
     primer_bind     2649..2665
                     /label=oYK09_lpl112-F
     primer_bind     2695..2710
                     /label=oYK10_lpl127-F
     primer_bind     2740..2758
                     /label=oYK11_lpl142-F
     primer_bind     2776..2794
                     /label=oYK12_lpl154-F
     primer_bind     2839..2861
                     /label=oYK13_lpl175-F
     primer_bind     2887..2905
                     /label=oYK14_lpl191-F
     primer_bind     2950..2965
                     /label=oYK15-lpl212-F
     primer_bind     2976..2995
                     /label=oYK16_lpl221-F
     primer_bind     3034..3055
                     /label=oYK17_lpl240-F
     primer_bind     3058..3076
                     /label=oYK18_lpl248-F
     primer_bind     3094..3108
                     /label=oYK19_lpl260-F
                     /note="Maybe mismatch"
     primer_bind     3130..3148
                     /label=oYK20_lpl272-F
     primer_bind     3161..3177
                     /label=oYK21_lpl283-F
     primer_bind     3208..3225
                     /label=oYK22_lpl298-F
                     /note="DNA may less than 100 bp"
     primer_bind     complement(3308..3327)
                     
     primer_bind     complement(3310..3327)
                     /label=oYK404_LplA_R
     primer_bind     complement(3312..3327)
                     /label=oYK228_lplA_R
     primer_bind     complement(3312..3327)
                     /label=oYK341_lplA_R
     primer_bind     complement(3312..3327)
                     /label=oYK372_LplA_R
     primer_bind     complement(3312..3327)
                     /label=oYK808_LplA_R
     primer_bind     complement(3317..3337)
                     /label=oYK01-1-R
     primer_bind     complement(3327..3351)
                     /label=oYK08-22-LplApart-R
     primer_bind     complement(3330..3351)
                     /label=oYK36-37-LplA-R
     primer_bind     complement(3332..3354)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(3356..3375)
                     /label=oSCL1089
     terminator      3428..3655
                     /label=bGH PA
     misc_feature    3701..4129
                     /label=f1 origin
     misc_feature    4134..4477
                     /label=SV40 early promoter and origin
     misc_feature    4323..4400
                     /label=SV40 ORI
     CDS             4542..5333
                     /label=Kan/neoR
     misc_feature    5507..5637
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(5673..5693)
                     /label=M13-rev
     misc_binding    complement(5699..5721)
                     /label=LacO
     promoter        complement(5726..5755)
                     /label=lac
     misc_feature    complement(6020..6693)
                     /label=pUC origin
     CDS             complement(6841..7500)
                     /label=AmpR
     primer_bind     7157..7168
                     /label=oyk03-03-F
     primer_bind     7157..7168
                     /label=oYK04-ns3a-F
     promoter        complement(7740..7768)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatga agaagaaggg cagcgtggtg atcgtgggca gaatcaacct gagcggcgac
      961 accgcctaca gccagcagac cagaggcctg gagggctgcc aggagaccag ccagaccggc
     1021 agagacaaga accaggtgga gggcgaggtg caggtggtga gcaccgccac ccagagcttc
     1081 ctggccacca gcatcaacgg cgtgctgtgg accgtgtacc acggcgccgg caccagaacc
     1141 atcgccagcc ccaagggccc cgtgacccag atgtacacga acgtggacaa ggacttagtg
     1201 ggctggcagg ccccccaagg cagcagaagc ctgaccccct gcacctgcgg cagcagcgac
     1261 ctgtacctgg tgaccagaca cgccgacgtg atccccgtga gaggaagagg cgacagcaga
     1321 ggcagcctgc tgagccccag acccatcagc tatctgaaag gctcaagcgg cggccccctg
     1381 ttgtgtcccg ccggccatgc cgtgggcatc ttcagagccg ccgtgagcac cagaggcgtg
     1441 gccaaggccg tggacttcat ccccgtggag agcctggaga ccaccatgag aagccccgct
     1501 agcagcggaa gtggaactgg gtctgggact gggtccggca ccgggactac ttcaggcacc
     1561 ggcactgggg gctccactgg catggtgagc gtgatcaagc ccgagatgaa gatcaagctg
     1621 tgcatgaggg gcaccgtgaa cggccacaac ttcgtgatcg agggcgaggg caagggcaac
     1681 ccctacgagg gcacccagat cctggacctg aacgtgaccg agggcgcccc cctgcccttc
     1741 gcctacgaca tcctgaccac cgtgttccag tacggcaaca gggccttcac caagtacccc
     1801 gccgacatcc aggactactt caagcagacc ttccccgagg gctaccactg ggagaggagc
     1861 atgacctacg aggaccaggg catctgcacc gccaccagca acatcagcat gaggggcgac
     1921 tgcttcttct acgacatcag gttcgacggc gtgaacttcc cccccaacgg ccccgtgatg
     1981 cagaagaaga ccctgaagtg ggagcccagc accgagaaga tgtacgtgag ggacggcgtg
     2041 ctgaagggcg acgtgaacat ggccctgctg ctggagggcg gcggccacta caggtgcgac
     2101 ttcaagacca cctacaaggc caagaaggac gtgaggctgc ccgactacca cttcgtggac
     2161 cacaggatcg agatcctgaa gcacgacaag gactacaaca aggtgaagct gtacgagaac
     2221 gccgtggcca ggtacagcat gctgcccagc caggccaagg gtaccggaac tgcagcagag
     2281 aattcgggaa actcgagaac aaagcttgaa atgggctcca cattacgcct gctcatctct
     2341 gactcttacg acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc
     2401 gccacgcagc gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg
     2461 cagaacccgt ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg
     2521 cggcgcagta gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt
     2581 atggctggca agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg
     2641 aacgcgctcg gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc
     2701 gaaggcgacc gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac
     2761 cacggcacct tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat
     2821 aaaaagaaac tggcggcgaa aggcattacg tcggtacgtt cccgcgtgac caacctcacc
     2881 gagctgttgc cggggatcac ccatgagcag gtttgcgagg ccataaccga ggcctttttc
     2941 gcccattatg gcgagcgcgt ggaagcggaa atcatctccc cgaacaaaac gccagacttg
     3001 ccaaacttcg ccgaaacctt tgcccgccag agtagctggg aatggaactt cggtcaggct
     3061 ccggcattct cgcatctgct ggatgaacgc tttacctggg gcggcgtgga actgcatttc
     3121 gacgttgaaa aaggccatat cacccgcgcc caggtgttta ccgacagcct caaccccgcg
     3181 ccgctggaag ccctcgccgg acgactgcaa ggctgcctgt accgcgcaga tatgctgcaa
     3241 caggagtgcg aagcgctgtt ggttgacttc ccggaacagg aaaaagagct acgggagtta
     3301 tcggcatgga tggcgggggc tgtaaggtag gggatccact agtccagtgt ggtggaattc
     3361 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
     3421 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3481 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3541 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3601 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3661 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3721 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3781 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3841 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3901 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3961 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4021 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4081 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4141 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4201 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4261 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4321 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4381 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4441 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4501 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4561 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4621 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4681 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4741 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4801 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4861 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4921 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4981 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5041 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     5101 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5161 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5221 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5281 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5341 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5401 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5461 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5521 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5581 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5641 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5701 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5761 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5821 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5881 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5941 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6001 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6061 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6181 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6481 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6541 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6601 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6661 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6721 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6781 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6841 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6901 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6961 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7021 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7081 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7141 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7201 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7261 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7321 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7381 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7441 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7501 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7561 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7621 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7681 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7741 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7801 gcgcacattt ccccgaaaag tgccacctga cgtc
//