| 质粒名称: | pSIN4-EF2-ABCG2-IRES-Neo |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 25983 |
| 存储实验室: | Ren-he Xu |
|
载体骨架基本信息 |
|
| 载体名称: | pSIN-EF2-MCS-IRES-Neo |
| 载体出品公司: | James Thomson |
| 空载体大小: | 6500 bp |
| 完整质粒大小: | 9576 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 新霉素（G418筛选） |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1968 bp |
| 基因突变: | -- |
| GenBank编号 | AAH92408.1 |
| 启动子: | -- |
| 标签/融合蛋白: | EF1 apha启动子（骨架上的N末端）IRES Neo（主干网上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoR I(未知是否破坏) |
| 3’克隆位点: | BamH I (未知是否破坏) |
| 5’测序引物 | EF-1a-F |
| 3’测序引物 | IRES-R |
|
文献引用方法 |
|
| 材料和方法部分: | pSIN4-EF2-ABCG2-IRES-Neo was a gift from Ren-he Xu (Addgene plasmid # 25983 ; http://n2t.net/addgene:25983 ; RRID:Addgene_25983) |
| 参考文献部分: | Lack of ABCG2 expression and side population properties in human pluripotent stem cells. Zeng H, Park JW, Guo M, Lin G, Crandall L, Compton T, Wang X, Li XJ, Chen FP, Xu RH. Stem Cells. 2009 Oct . 27(10):2435-45. 10.1002/stem.192 PubMed 19670287 |
买家导航
pSIN4-EF2-ABCG2-IRES-Neo载体质粒基本信息
pSIN4-EF2-ABCG2-IRES-Neo质粒图谱载体图谱和pSIN4-EF2-ABCG2-IRES-Neo载体序列质粒序列多克隆位点信息
pSIN4-EF2-ABCG2-IRES-Neo质粒载体简介
pSIN4-EF2-ABCG2-IRES-Neo质粒序列载体序列
LOCUS pSIN4-EF2-ABCG2-IRES-Neo 9576 bp ds-DNA circular SYN 16-JUN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSIN4-EF2-ABCG2-IRES-Neo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9576)
AUTHORS Zeng H, Park JW, Guo M, Lin G, Crandall L, Compton T, Wang X, Li XJ,
Chen FP, Xu RH
TITLE Lack of ABCG2 expression and side population properties in human
pluripotent stem cells.
JOURNAL Stem Cells. 2009 Oct . 27(10):2435-45.
PUBMED 19670287
REFERENCE 2 (bases 1 to 9576)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 16, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9576
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(159..176)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 386..405
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 601..1404
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(664..683)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 1274..1293
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
LTR 1607..1840
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(1991..2008)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1997..2221
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
regulatory 2264..2273
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter 2317..2421
/gene="bla"
/label=AmpR promoter
CDS 2422..3282
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2640..2659)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3453..4041
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3942..3961
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
LTR 4200..4833
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 4880..5005
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5498..5731
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5916..5960
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 6247..6364
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6493..7671
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 6724..7662
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 7619..7639
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
misc_feature 9568..565
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
ORIGIN
1 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
61 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
121 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
181 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
241 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
301 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
361 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
421 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
481 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
541 tttcctttga aaaacacgat gataagcttg ccacaacccg ggataattcc tgcagccaat
601 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
661 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
721 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
781 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
841 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
901 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
961 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
1021 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
1081 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
1141 atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
1201 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
1261 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
1321 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
1381 cgccttcttg acgagttctt ctgataatag gcggccgctc gagacctaga aaaacatgga
1441 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
1501 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
1561 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
1621 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
1681 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
1741 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
1801 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
1861 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
1921 tataatagat ttagcaggaa ttgaactagg agtggagcac acaggcaaag ttctagagct
1981 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
2041 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
2101 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
2161 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
2221 gcttctgagg cggaaagaac cagctggggg cgcgcccctc gaggccgcca tggtcatagc
2281 tgtttgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
2341 ttctaaatac attcaaatat gtatccgctc acgagacaat aaccctgata aatgcttcaa
2401 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
2461 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
2521 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
2581 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
2641 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
2701 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
2761 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
2821 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
2881 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
2941 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
3001 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
3061 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
3121 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
3181 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
3241 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
3301 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
3361 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
3421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
3481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
3541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
3601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
3661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
3721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
3781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
3841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
3901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
3961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
4021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt ctggcctttt
4081 gcacggaccg ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
4141 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
4201 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
4261 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
4321 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
4381 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
4441 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
4501 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
4561 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
4621 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
4681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
4741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
4801 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
4861 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
4921 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
4981 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
5041 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
5101 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
5161 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
5221 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
5281 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
5341 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
5401 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
5461 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
5521 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
5581 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
5641 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
5701 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
5761 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
5821 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
5881 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
5941 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
6001 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
6061 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
6121 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
6181 acagatccat tcgattagtg aacggatctc gacggtatcg ccacaaatgg cagtattcat
6241 ccacaatttt aaaagaaagg gggggattgg ggggtacagt gcaggggaaa gaatagtaga
6301 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
6361 ttttcgggtt tattacaggg acagcagaga tccactttgg atcgataagc tttgcaaaga
6421 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
6481 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
6541 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
6601 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
6661 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
6721 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
6781 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
6841 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
6901 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
6961 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
7021 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
7081 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
7141 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
7201 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
7261 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
7321 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
7381 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
7441 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
7501 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
7561 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
7621 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattcgc
7681 caccatgtct tccagtaatg tcgaagtttt tatcccagtg tcacaaggaa acaccaatgg
7741 cttccccgcg acagcttcca atgacctgaa ggcatttact gaaggagctg tgttaagttt
7801 tcataacatc tgctatcgag taaaactgaa gagtggcttt ctaccttgtc gaaaaccagt
7861 tgagaaagaa atattatcga atatcaatgg gatcatgaaa cctggtctca acgccatcct
7921 gggacccaca ggtggaggca aatcttcgtt attagatgtc ttagctgcaa ggaaagatcc
7981 aagtggatta tctggagatg ttctgataaa tggagcaccg cgacctgcca atttcaaatg
8041 taattcaggt tacgtggtac aagatgatgt tgtgatgggc actctgacgg tgagagaaaa
8101 cttaaagttc tcagcagctc ttcggcttgc aacaactatg acgaatcatg aaaaaaacga
8161 acggattaac agggtcattc aagagttagg tctggataaa gtggcagact ccaaggttgg
8221 aactcagttt atccgtggtg tgtctggagg agaaagaaaa aggactagta taggaatgga
8281 gcttatcact gatccttcca tcttgttctt ggatgagcct acaactggct tagactcaag
8341 cacagcaaat gctgtccttt tgctcctgaa aaggatgtct aagcagggac gaacaatcat
8401 cttctccatt catcagcctc gatattccat cttcaagttg tttgatagcc tcaccttatt
8461 ggcctcagga agacttatgt tccacgggcc tgctcaggag gccttgggat actttgaatc
8521 agctggttat cactgtgagg cctataataa ccctgcagac ttcttcttgg acatcattaa
8581 tggagattcc actgctgtgg cattaaacag agaagaagac tttaaagcca cagagatcat
8641 agagccttcc aagcaggata agccactcat agaaaaatta gcggagattt atgtcaactc
8701 ctccttctac aaagagacaa aagctgaatt acatcaactt tccgggggtg agaagaagaa
8761 gaagatcaca gtcttcaagg agatcagcta caccacctcc ttctgtcatc aactcagatg
8821 ggtttccaag cgttcattca aaaacttgct ggttaatccc caggcctcta tagctcagat
8881 cattgtcaca gtcgtactgg gactggttat aggtgccatt tactttgggc taaaaaatga
8941 ttctactgga atccagaaca gagctggggt tctcttcttc ctgacgacca accagtgttt
9001 cagcagtgtt tcagccgtgg aactctttgt ggtagagaag aagctcttca tacatgaata
9061 catcagcgga tactacagag tgtcatctta tttccttgga aaactgttat ctgatttatt
9121 acccatgagg atgttaccaa gtattatatt tacctgtata gtgtacttca tgttaggatt
9181 gaagccaaag gcagatgcct tcttcgttat gatgtttacc cttatgatgg tggcttattc
9241 agccagttcc atggcactgg ccatagcagc aggtcagagt gtggtttctg tagcaacact
9301 tctcatgacc atctgttttg tgtttatgat ggtctgttgg tcaatctcac aaccattgca
9361 tcttggctgt catggcttca gtacttcagc attccacgat atggatttac ggctttgcag
9421 cataatgaat ttttgggaca aaacttctgc ccaggactca atgcaacagg aaacaatcct
9481 tgtaactatg caacatgtac tggcgaagaa tatttggtaa ggatccgcat gcatctaggg
9541 cggccaattc cgcccctctc cccccccccc ctctcc
//
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pSIN4-EF2-ABCG2-IRES-Neo质粒载体应用举例
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