pLV-puro-N-3HA-SREBP1-C-Flag

LOCUS       pLV-puro-N-3HA-SREBP1-C-Flag               12357 bp ds-DNA     circular SYN 19-MAR-2020
DEFINITION  Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-puro-N-3HA-SREBP1-C-Flag
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12357)
  AUTHORS   Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X,
            Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, 
            Moresco EMY, Beutler B
  TITLE     Skin-specific regulation of SREBP processing and lipid biosynthesis 
            by glycerol kinase 5.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 
            10.1073/pnas.1705312114. Epub 2017 Jun 12.
  PUBMED    28607088
REFERENCE   2  (bases 1 to 12357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2020 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12357
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1810..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2131..3312
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2361..3303
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3260..3280
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3351..3377
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3384..3410
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3414..3440
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6864..6887
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6988..7561
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7011..7563
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7155..7172)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7382..7401
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7595..8194
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7595..7614)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8091..8111
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8209..8797
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8262..8282)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8680..8691)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8884..9117
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9189..9310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9226..9245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9280..9299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9350..9485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9412..9431
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9505..9524)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9506..9524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9534..9551)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9534..9550)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9543..9565)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9692..10147
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9779..9798)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9989..10010
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10173..10277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10278..11138
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10496..10515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11309..11897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11798..11817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12051..12068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12185..12206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12221..12251
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12259..12275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12264..12286
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12283..12299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12283..12299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12318..12338
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12320..12338
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcttgggc gctggggccg ccgcgtgcga
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2641 ttttgatgat atcctgcgac gctttttttt tggcaagata gtcttgtaaa tgcgggccaa
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3301 caggtgtcgt gaaaactacc cctgagctcc ttaaggttaa cgccaccatg tacccttatg
     3361 acgtacctga ctatgctggc gcctaccctt atgacgtacc tgactatgct ggataccctt
     3421 atgacgtacc tgactatgct ggatctagag aattcggatc catggacgag ctggccttcg
     3481 gtgaggcggc tctggaacag acactggccg agatgtgcga actggacaca gcggttttga
     3541 acgacatcga agacatgctc cagctcatca acaaccaaga cagtgacttc ccgggcctgt
     3601 ttgacgcccc ctatgctggg ggtgagacag gggacacagg ccccagcagc ccaggtgcca
     3661 actctcctga gagcttctct tctgcttctc tggcctcctc tctggaagcc ttcctgggag
     3721 gacccaaggt gacacctgca cccttgtccc ctccaccatc ggcacccgct gctttaaaga
     3781 tgtacccgtc cgtgtccccc ttttcccctg ggcctgggat caaagaggag ccagtgccac
     3841 tcaccatcct acagcctgca gcgccacagc cgtcaccggg gaccctcctg cctccgagct
     3901 tccccgcacc acccgtacag ctcagccctg cgcccgtgct gggttactcg agcctgcctt
     3961 caggcttctc agggaccctt ccaggaaaca ctcagcagcc accatctagc ctgccgctgg
     4021 cccctgcacc aggagtcttg cccacccctg ccctgcacac ccaggtccaa agcttggcct
     4081 cccagcagcc gctgccagcc tcagcagccc ctagaacaaa cactgtgacc tcacaggtcc
     4141 agcaggtccc agttgtactg cagccacact tcatcaaggc agactcactg ctgctgacag
     4201 ctgtgaagac agatgcagga gccaccatga agactgcagg catcagcacc ctggctcctg
     4261 gcacagccgt gcaggcaggt cccctgcaga ccctggtgag tggagggacc atcttggcca
     4321 cagtaccttt ggttgtggac acagacaaac tgcccatcca ccgactcgca gctggcagca
     4381 aggccctagg ctcagctcag agccgtggtg agaagcgcac agcccacaat gccattgaga
     4441 agcgctaccg gtcctctatc aatgacaaga ttgtggagct caaagacctg gtggtgggca
     4501 ctgaagcaaa gctgaataaa tctgctgtct tgcgcaaggc catcgactac atccgcttct
     4561 tgcagcacag caaccagaag ctcaagcagg agaacctgac cctacgaagt gcacacaaaa
     4621 gcaaatcact gaaggacctg gtgtcagctt gtggcagtgg aggaggcaca gatgtgtcta
     4681 tggagggcat gaaacccgaa gtggtggaga cgcttacccc tccaccctca gacgccggct
     4741 caccctccca gagtagcccc ttgtcttttg gcagcagagc tagcagcagt ggtggtagtg
     4801 actctgagcc cgacagtcca gcctttgagg atagccaggt caaagcccag cggctgcctt
     4861 cacacagccg aggcatgctg gaccgctccc gcctggccct gtgtgtactg gcctttctgt
     4921 gtctgacctg caatcctttg gcctcgcttt tcggctgggg cattctcact ccctctgatg
     4981 ctacgggtac acaccgtagt tctgggcgca gcatgctgga ggcagagagc agagatggct
     5041 ctaattggac ccagtggttg ctgccacccc tagtctggct ggccaatgga ctactagtgt
     5101 tggcctgctt ggctcttctc tttgtctatg gggaacctgt gactaggcca cactctggcc
     5161 cggctgtaca cttctggaga catcgcaaac aagctgacct ggatttggcc cggggagatt
     5221 tcccccaggc tgctcaacag ctgtggctgg ccctgcaagc gctgggccgg cccctgccca
     5281 cctcaaacct ggatctggcc tgcagtctgc tttggaacct catccgccac ctgctccagc
     5341 gtctctgggt gggccgctgg ctggcaggcc aggccggggg cctgctgagg gaccgtgggc
     5401 tgaggaagga tgcccgtgcc agtgcccggg atgcggctgt tgtctaccat aagctgcacc
     5461 agctgcatgc catgggcaag tacacaggag gacatcttgc tgcttctaac ctggcactaa
     5521 gtgccctcaa cctggctgag tgcgcaggag atgctatctc catggcaaca ctggcagaga
     5581 tctatgtggc agcggccctg agggtcaaaa ccagcctccc aagagccctg cacttcttga
     5641 cacgtttctt cctgagcagc gcccgccagg cctgcctagc acagagcggc tcggtgcctc
     5701 ttgccatgca gtggctctgc caccctgtag gtcaccgttt ctttgtggac ggggactggg
     5761 ccgtgcacgg tgcccccccg gagagcctgt acagcgtggc tgggaaccca gtggatccgc
     5821 tggcccaggt gacccggcta ttccgtgaac atctcctaga gcgagcgttg aactgtattg
     5881 ctcagcccag cccaggggca gctgacggag acagggagtt ctcagatgcc cttggatatc
     5941 tgcagttgct aaatagctgt tctgatgctg ccggggctcc tgcttgcagt ttctctgtca
     6001 gctccagcat ggctgccacc actggcccag acccagtggc caagtggtgg gcctcactga
     6061 cagctgtggt gatccactgg ctgaggcggg atgaagaggc agctgagcgc ttgtacccac
     6121 tggtagagca tatcccccag gtgctgcagg acactgagag acccctgccc agggcagctc
     6181 tgtactcctt caaggctgcc cgggctctgc tggaccacag aaaggtggaa tctagcccag
     6241 ccagcctggc catctgtgag aaggccagtg ggtacctgcg ggacagctta gcctctacac
     6301 caactggcag ttccattgac aaggccatgc agctgctcct gtgtgatcta cttcttgtgg
     6361 cccgtaccag tctgtggcag cggcagcagt caccagcttc agtccaggta gctcacggta
     6421 ccagcaatgg accccaggcc tctgctctgg agctgcgtgg tttccaacat gacctgagca
     6481 gcctgcggcg gttggcacag agcttccggc ctgctatgag gagggtattc ctacatgagg
     6541 ccacagctcg gctgatggca ggagcaagtc ctgcccggac acaccagctc ctggatcgca
     6601 gtctgaggag gagggcaggt tccagtggca aaggaggcac tacagctgag ctggagccac
     6661 ggcccacatg gcgggagcac accgaggccc tgctgttggc atcctgctat ctgccccctg
     6721 ccttcctgtc ggctcctggg cagcgaatga gcatgctggc cgaggcggca cgcaccgtag
     6781 agaagcttgg cgatcaccgg ctactgctgg actgccagca gatgctcctg cgcctgggcg
     6841 gcggaaccac cgtcacttcc agcgactaca aagacgatga cgacaagtag cccgggctcg
     6901 agccatggaa gcttgatatc taactgactg aaccggtggt accgatccac gcgtctccgg
     6961 cctagggata acagggtaat ccgctagccc ctctccctcc ccccccccta acgttactgg
     7021 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     7081 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7141 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     7201 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     7261 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     7321 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     7381 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     7441 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa
     7501 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     7561 atatggccac actagagatc caccggtcgc caccatgacc gagtacaagc ccacggtgcg
     7621 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     7681 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     7741 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7801 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7861 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7921 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7981 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8041 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8101 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8161 cacctggtgc atgacccgca agcccggtgc ctgagcggcc gcgtcgacaa tcaacctctg
     8221 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8281 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8341 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8401 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8461 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8521 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8581 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     8641 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8701 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8761 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     8821 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8881 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     8941 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9001 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9061 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9121 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9181 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9241 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9301 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9361 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9421 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9481 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9541 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9601 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9661 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     9721 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9781 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9841 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9901 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9961 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    10021 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10081 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    10141 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12121 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12181 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12241 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12301 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgc
//