| 质粒名称: | pCx43-7-miRFP670-2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000073 |
| 产品用途: | Expresses miRFP670-2 fused to C-terminus of human connexin 43 |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Cx43, miRFP670-2 |
| 物种来源: | Homo sapiens, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pCx43-7-miRFP670-2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCx43-7-miRFP670-2载体质粒基本信息
pCx43-7-miRFP670-2质粒图谱载体图谱和pCx43-7-miRFP670-2载体序列质粒序列多克隆位点信息
pCx43-7-miRFP670-2质粒载体简介
pCx43-7-miRFP670-2质粒序列载体序列
LOCUS pCx43-7-miRFP670-2 6071 bp DNA circular SYN 21-APR-2023
DEFINITION Vector for fusing EGFP to the C-terminus of a partner protein. For
other reading frames, use pEGFP-N2 or pEGFP-N3.
ACCESSION U55762
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6071)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Jun 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6071
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 635..1780
/codon_start=1
/label=Cx43-7
/translation="MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAW
GDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEE
KLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKS
VFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLA
LNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPM
SPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDD
NQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI"
primer_bind 1774..1824
/label=0416-PM639-F
CDS 1802..2734
/codon_start=1
/product="near-infrared fluorescent protein with an
emission peak at 670 nm, engineered from a bacterial
phytochrome (Shcherbakova and Verkhusha, 2013)"
/label=miRFP670-2
/note="derived from Rhodopseudomonas palustris RpBphP6"
/translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
PRAVPMAQRVAAKRFAERLSTHFTAAHHQR"
regulatory 1802..1805
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(2715..2760)
/label=0416-PM639-R
polyA_signal 2859..2980
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2987..3442)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3469..3573
/gene="bla"
/label=AmpR promoter
promoter 3575..3932
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3783..3918
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3967..4761
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4993..5040
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 5369..5957
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attcatgggt gactggagtg ccttggggaa
661 gcttctggac aaggtccaag cctactccac cgctggaggg aaggtgtggc tgtcagtgct
721 cttcatattc agaatcctgc tcctggggac agctgttgag tcagcttggg gtgatgaaca
781 gtctgccttt cgctgtaaca ctcaacaacc tggctgcgaa aacgtctgct atgacaagtc
841 cttccccatc tctcacgtgc gcttctgggt ccttcagatc atattcgtgt ctgtgcccac
901 actcctgtac ttggcccatg tgttctatgt gatgaggaag gaagagaagc taaacaagaa
961 agaagaggag ctcaaagtgg cccagactga cggggtcaac gtggagatgc acctgaagca
1021 gattgaaatc aagaagttca agtacgggat tgaagagcac ggcaaggtga aaatgagggg
1081 cggcttgctg agaacctaca tcatcagcat cctcttcaag tctgtcttcg aggtggcctt
1141 cctgctcatc cagtggtaca tctatgggtt cagcttgagc gcggtctaca cctgcaagag
1201 agatccctgc ccccaccagg tagactgctt cctctcacgt cccacggaga aaaccatctt
1261 catcatcttc atgctggtgg tgtccttggt gtctctcgct ttgaacatca ttgagctctt
1321 ctacgtcttc ttcaagggcg ttaaggatcg cgtgaaggga agaagcgatc cttaccacgc
1381 caccactggc ccactgagcc catcaaaaga ctgcggatct ccaaaatacg cctacttcaa
1441 tggctgctcc tcaccaacgg ctccactctc gcctatgtct cctcctgggt acaagctggt
1501 tactggtgac agaaacaatt cctcgtgccg caattacaac aagcaagcta gcgagcaaaa
1561 ctgggcgaac tacagcgcag agcaaaatcg catggggcag gccggaagca ccatctccaa
1621 ctcgcacgcc cagccgttcg atttccccga cgacaaccag aatgccaaaa aagttgctgc
1681 tggacatgaa ctccagccat tagccatcgt ggaccaacga ccttccagca gagccagcag
1741 ccgcgccagc agcaggcctc ggcctgatga cctggagatt gcggatccac cggtcgccac
1801 catggcgcgt aaggtcgatc tcacctcctg cgatcgcgag ccgatccaca tccccggcag
1861 cattcagccg tgcggctgcc tgctagcctg cgacgcgcag gcggtgcgga tcacgcgcat
1921 tacggaaaat gccggcgcgt tctttggacg cgaaactccg cgggtcggtg agctactcgc
1981 cgattacttc ggcgagaccg aagcccatgc gctgcgcaac gcactggcgc agtcctccga
2041 tccaaagcga ccggcgctga tcttcggttg gcgcgacggc ctgaccggcc gcaccttcga
2101 catctcactg catcgccatg acggtacatc gatcatcgag ttcgagcctg cggcggccga
2161 acaggccgac aatccgctgc ggctgacgcg gcagatcatc gcgcgcacca aagaactgaa
2221 gtcgctcgaa gagatggccg cacgggtgcc gcgctatctg caggcgatgc tcggctatca
2281 ccgcgtgatg ttgtaccgct tcgcggacga cggctccggg atggtgatcg gcgaggcgaa
2341 gcgcagcgac ctcgagagct ttctcggtca gcactttccg gcgtcgctgg tcccgcagca
2401 ggcgcggcta ctgtacttga agaacgcgat ccgcgtggtc tcggattcgc gcggcatcag
2461 cagccggatc gtgcccgagc acgacgcctc cggcgccgcg ctcgatctgt cgttcgcgca
2521 cctgcgcagc atctcgccct gccatctcga atttctgcgg aacatgggcg tcagcgcctc
2581 gatgtcgctg tcgatcatca ttgacggcac gctatgggga ttgatcatct gtcatcatta
2641 cgagccgcgt gccgtgccga tggcgcagcg cgtcgcggcc aaaaggttcg ccgagcgctt
2701 atcgacgcac ttcaccgccg cccaccacca acgctaagcg gccgcgactc tagatcataa
2761 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
2821 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
2881 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
2941 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
3001 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
3061 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
3121 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
3181 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
3241 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
3301 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
3361 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
3421 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
3481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
3541 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
3601 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
3661 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
3721 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
3781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
3841 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
3901 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
3961 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
4021 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
4081 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
4141 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
4201 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
4261 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
4321 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
4381 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
4441 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
4501 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
4561 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
4621 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
4681 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
4741 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
4801 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
4861 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
4921 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
4981 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
5041 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
5101 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
5161 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
5221 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
5281 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
5341 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
5401 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
5461 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
5521 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
5581 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
5641 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
5701 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
5761 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
5821 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
5881 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
5941 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
6001 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
6061 ccgccatgca t
//
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