Lenti-mEGFP-mmG3BP1

LOCUS       Lenti-mEGFP-mmG3BP1              10327 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3497..4894
                     /codon_start=1
                     /label=mmG3BP1
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYAHGGL
                     DSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQA
                     LRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTP
                     EVVPDDSGTFYDQTVSNDLEEHLEEPVVEPEPEPEPEPEPEPVSDIQEDKPEAALEEAA
                     PDDVQKSTSPAPADVAPAQEDLRTFSWASVTSKNLPPSGAVPVTGTPPHVVKVPASQPR
                     PESKPDSQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEPGDVEPRRMVRHPDSHQL
                     FIGNLPHEVDKSELKDFFQNFGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIM
                     FRGAVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGPSGGMRGPPRGGMVQKPGFGVGR
                     GITTPRQ"
     promoter        4905..5404
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5431..6225
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    6249..6837
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6720..6731)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7045..7678
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7807..7823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7831..7847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7855..7885)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7900..7921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8209..8794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8965..9825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9826..9930)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9978..10112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3481 ctcagatctc gagctcatgg ttatggagaa gcctagtccc ctgctggtcg ggcgggagtt
     3541 tgtgcgacag tactacactc tgctgaacca ggccccggac atgttgcaca gattttatgg
     3601 aaagaactct tcctatgccc acgggggctt ggattccaac gggaagccag cagatgcagt
     3661 ctacgggcag aaggaaatcc acaggaaagt gatgtcacaa aacttcacca actgccacac
     3721 caagatccgc cacgtggatg ctcacgcaac tctgaatgac ggggtggtgg tccaagtgat
     3781 ggggctactg tccaacaaca accaggctct gcggaggttc atgcagacct ttgtccttgc
     3841 tcctgagggc tctgttgcca acaaattcta tgttcacaac gacatcttca ggtaccaaga
     3901 tgaggtcttc ggtggctttg tcacagagcc tcaagaggaa tccgaggaag aagtagaaga
     3961 acctgaagaa agacagcaga caccagaggt ggtgcctgat gattctggaa ctttctatga
     4021 tcagactgtc agcaatgact tggaggagca tttagaggag cctgtagtgg aaccagaacc
     4081 ggagccagag ccggagccgg agccggaacc tgtgtccgac attcaagagg acaagcctga
     4141 ggctgcattg gaagaagctg ctcccgacga tgtgcagaag agcacttccc cggccccggc
     4201 tgatgtggcc ccggcacagg aggacttgag gacgttttct tgggcatctg tgacgagtaa
     4261 gaaccttcct cccagtgggg ctgttccagt gacggggaca ccacctcatg tggtcaaagt
     4321 gccagcgtca cagccccgtc cagaatctaa acctgactct cagatcccac cacaaaggcc
     4381 tcagagagat cagagagttc gagagcagcg aatcaatatc cctcctcaga gaggaccccg
     4441 accaatccgt gaggctggtg aaccaggaga tgtggaacct cgaaggatgg tgaggcaccc
     4501 tgacagtcac cagctcttca ttgggaacct gccacatgag gttgacaagt cggaactgaa
     4561 ggattttttc caaaattttg gcaatgtggt ggagctgcgc atcaacagtg gtgggaagtt
     4621 acccaatttc ggttttgttg tgtttgatga ttctgaacct gttcagaagg tccttagtaa
     4681 taggcccatc atgttccgag gcgcggtccg cctgaatgtg gaagagaaga agactcgagc
     4741 tgccagagaa ggcgaccgca gggataaccg ccttcgggga cctggaggcc cccgtggtgg
     4801 gccgagtggt gggatgagag gccctccccg cggaggcatg gtgcagaaac caggctttgg
     4861 cgtgggcagg gggattacaa ctccaaggca gtgagaattc taccgggtag gggaggcgct
     4921 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     4981 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     5041 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     5101 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     5161 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     5221 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     5281 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     5341 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     5401 ttcgacctgc agcccaagct tcctaggacc atgattgaac aagatggatt gcacgcaggt
     5461 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5521 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5581 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     5641 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5701 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5761 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5821 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5881 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5941 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6001 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6061 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6121 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6181 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgacccgg gcggggcgcg
     6241 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6301 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6361 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6421 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6481 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6541 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6601 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6661 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6721 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6781 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6841 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6901 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggtttttc
     6961 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7021 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     7081 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7141 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7201 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7261 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7321 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     7381 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7441 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     7501 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7561 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7621 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7681 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7741 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     7801 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     8761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8881 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8941 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9001 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9061 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9241 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9301 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9361 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9421 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9481 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9541 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9601 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9661 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9721 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9781 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9841 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9901 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9961 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10021 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10081 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10141 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta
    10201 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10261 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10321 tagtagt
//