pGEX-2T-drG3BP2

LOCUS       pGEX-2T-drG3BP2                6474 bp DNA     circular SYN 09-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             918..938
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             942..2465
                     /codon_start=1
                     /label=drG3BP2 codon
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNKAPDFLHRFYGRNSSYVHGGL
                     DLNGKLSEAVYGQAEIHKKVMSLQFSECHTKIRHVDAHATLSDGVVVQVMGELSNNGQP
                     MRKFLQTFVLAPEGSVANKFYVHNDIFRYEDEVFGDSEAELDEESEEETDEPEDRQPSP
                     EPLQDSPSNANCYEPHPVSCNNGVEEAHEEAVMDLQSEVAPDPKIEESKLKVEEKDVEE
                     FEEKALSPVPLESPPNIQEPPKTSSWASVTSKNLPLSGTVPSAGGQPHVVKAPNTQPRV
                     ETKPEMLSASIRPRDQRIRDRPPVTSRGPRSDGVPSSEPQAGKPHFSFVNKGRADESSE
                     MDSRRVVRYPDSHQLFVGNLPHDIDESELKDFFMTFGNVVELRINTKSTGGKIPNFGFV
                     VFDDSEPVQRILGVKPIMFRGEVRLNVEEKKTRAMRERETRSGVGDDRRDVRRSDRGPG
                     GPRGIAGGNMMRERDGRGPPSRVATVPKPGLASGRGSGSNEGRFNAQRR"
     promoter        2777..2881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2882..3742
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3913..4501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4745..4822
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4823..5905
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    5918..5939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5954..5984
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5992..6008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6016..6032
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6028..6474
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
     primer_bind     complement(6048..6064)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggttatg gaaaagccga
      961 gcccgctgct ggtgggtcgt gaatttgtgc gccagtatta taccctgctg aataaggcac
     1021 cggattttct gcatcgtttt tatggccgca atagtagcta tgtgcatggc ggcctggatc
     1081 tgaatggcaa actgagtgaa gcagtttatg gccaggcaga aattcataaa aaagtgatga
     1141 gcctgcagtt tagtgaatgt cataccaaaa ttcgccatgt ggatgcacat gcaaccctga
     1201 gcgatggcgt tgttgttcag gttatgggtg aactgagcaa taatggtcag ccgatgcgta
     1261 aatttctgca gacctttgtt ctggccccgg aaggcagcgt tgccaataag ttttatgttc
     1321 ataatgacat cttccgctat gaagatgaag tgtttggcga tagcgaagcc gaactggatg
     1381 aagaaagtga agaagaaacc gatgaaccgg aagatcgtca gccgagcccg gaaccgctgc
     1441 aggatagccc gagcaatgcc aattgttatg aaccgcatcc ggtgagttgc aataatggcg
     1501 tggaagaagc acatgaagaa gcagtgatgg atctgcagag tgaagtggca ccggacccta
     1561 aaattgaaga aagtaaactg aaagtggagg aaaaagatgt ggaagaattt gaagaaaagg
     1621 ccctgagtcc ggtgccgctg gaaagcccgc cgaatattca ggaaccgccg aaaaccagta
     1681 gctgggccag tgtgaccagc aaaaatctgc cgctgagcgg caccgtgccg agtgcaggtg
     1741 gccagcctca tgttgttaaa gccccgaata cccagccgcg tgtggaaacc aaaccggaaa
     1801 tgctgagcgc aagtattcgt ccgcgtgatc agcgtattcg tgatcgtccg ccggtgacca
     1861 gtcgcggccc tagaagtgat ggcgtgccga gtagtgaacc gcaggcaggc aaaccgcatt
     1921 ttagttttgt gaataagggt cgcgcagatg aaagcagtga aatggatagt cgtcgtgtgg
     1981 tgcgctatcc ggatagtcat cagctgtttg tgggcaatct gccgcatgat attgatgaaa
     2041 gtgaactgaa agatttcttc atgacctttg gtaatgttgt tgaactgcgt attaatacca
     2101 aaagcaccgg tggcaaaatt ccgaattttg gttttgttgt gttcgatgat agtgaaccgg
     2161 ttcagcgtat tctgggcgtt aaaccgatta tgtttcgtgg tgaagttcgc ctgaatgtgg
     2221 aagaaaagaa aacccgtgcc atgcgcgaac gcgaaacccg cagcggtgtg ggcgatgatc
     2281 gccgtgatgt tcgtcgcagt gatcgcggtc cgggcggccc tagaggcatt gcaggcggta
     2341 atatgatgcg tgaacgcgat ggccgcggtc cgccgagtcg tgttgccaca gtgccgaaac
     2401 cgggtctggc aagcggtcgc ggcagcggta gcaatgaagg ccgttttaat gcccagcgtc
     2461 gctaagaatt catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
     2521 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     2581 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     2641 gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
     2701 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2761 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2821 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2881 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2941 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3001 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3061 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3121 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3181 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3241 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3301 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3361 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3421 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3481 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3541 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3601 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3661 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3721 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3781 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3841 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4021 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4561 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4621 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4681 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa
     4741 ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     4801 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     4861 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     4921 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     4981 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     5041 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     5101 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     5161 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     5221 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     5281 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     5341 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     5401 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     5461 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     5521 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     5581 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     5641 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     5701 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     5761 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     5821 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5881 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5941 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6001 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
     6061 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6121 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6181 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     6241 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
     6301 cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
     6361 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
     6421 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aatt
//