| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA6.2/EmGFP-Bsd/V5-DEST |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | Gateway |
| 启动子: | CMV |
| 载体大小: | 8198 bp |
| 5' 测序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | V5 Epitope Tag |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 囊虫素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pcDNA6.2/EmGFP-Bsd/V5-DEST载体质粒基本信息
pcDNA6.2/EmGFP-Bsd/V5-DEST质粒图谱载体图谱和pcDNA6.2/EmGFP-Bsd/V5-DEST载体序列质粒序列多克隆位点信息
pcDNA6.2/EmGFP-Bsd/V5-DEST质粒载体简介
pcDNA6.2/EmGFP-Bsd/V5-DEST质粒序列载体序列
LOCUS pcDNA6.2 EmGFP-Bsd V5-DEST 8198 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION Vivid Colors(TM) Gateway(R) destination vector for expressing
proteins in mammalian cells, with an EmGFP-Bsd fusion as a
transfection marker.
ACCESSION .
VERSION .
KEYWORDS pcDNA6.2 EmGFP-Bsd V5-DEST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8198)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Saturday, June 20, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8198
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 911..1035
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR1"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(1464..1769)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(2114..2773)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(2774..2876)
/note="cat promoter"
/note="promoter of the E. coli cat gene"
protein_bind complement(3054..3178)
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 3204..3245
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
polyA_signal 3329..3377
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3579..4007
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4022..4521
/note="PGK promoter"
/note="mouse phosphoglycerate kinase 1 promoter"
promoter 4535..4582
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 4601..5317
/codon_start=1
/product="Emerald GFP"
/note="EmGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 5318..5713
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/note="BSD"
/note="confers resistance to blasticidin"
/translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
AIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 5871..5992
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(6443..7031)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7202..8062)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8063..8167)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagctatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
961 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
1021 tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt cgaccgacag
1081 ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc ttccaaatgt
1141 tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat gccgtattaa
1201 atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac aaaataaaaa
1261 catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca tcaagaacaa
1321 tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga ttttcagcct
1381 ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc tgcagactgg
1441 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
1501 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca
1561 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
1621 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
1681 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
1741 cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga
1801 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca
1861 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat
1921 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata
1981 cgctgcttca tagcacacct ctttttgaca tacttcgggt atacatatca gtatatattc
2041 ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg
2101 atccacgcgg cgtttacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
2161 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
2221 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
2281 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
2341 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
2401 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
2461 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
2521 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
2581 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
2641 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
2701 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
2761 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
2821 ccgg