| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA6.2/EmGFP-Bsd/V5-DEST |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | Gateway |
| 启动子: | CMV |
| 载体大小: | 8198 bp |
| 5' 测序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | V5 Epitope Tag |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 囊虫素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pcDNA6.2/EmGFP-Bsd/V5-DEST载体质粒基本信息
pcDNA6.2/EmGFP-Bsd/V5-DEST质粒图谱载体图谱和pcDNA6.2/EmGFP-Bsd/V5-DEST载体序列质粒序列多克隆位点信息
pcDNA6.2/EmGFP-Bsd/V5-DEST质粒载体简介
pcDNA6.2/EmGFP-Bsd/V5-DEST质粒序列载体序列
LOCUS pcDNA6.2 EmGFP-Bsd V5-DEST 8198 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION Vivid Colors(TM) Gateway(R) destination vector for expressing
proteins in mammalian cells, with an EmGFP-Bsd fusion as a
transfection marker.
ACCESSION .
VERSION .
KEYWORDS pcDNA6.2 EmGFP-Bsd V5-DEST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8198)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Saturday, June 20, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8198
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 911..1035
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR1"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(1464..1769)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(2114..2773)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(2774..2876)
/note="cat promoter"
/note="promoter of the E. coli cat gene"
protein_bind complement(3054..3178)
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 3204..3245
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
polyA_signal 3329..3377
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3579..4007
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4022..4521
/note="PGK promoter"
/note="mouse phosphoglycerate kinase 1 promoter"
promoter 4535..4582
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 4601..5317
/codon_start=1
/product="Emerald GFP"
/note="EmGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 5318..5713
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/note="BSD"
/note="confers resistance to blasticidin"
/translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
AIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 5871..5992
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(6443..7031)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7202..8062)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8063..8167)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagctatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
961 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
1021 tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt cgaccgacag
1081 ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc ttccaaatgt
1141 tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat gccgtattaa
1201 atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac aaaataaaaa
1261 catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca tcaagaacaa
1321 tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga ttttcagcct
1381 ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc tgcagactgg
1441 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
1501 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca
1561 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
1621 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
1681 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
1741 cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga
1801 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca
1861 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat
1921 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata
1981 cgctgcttca tagcacacct ctttttgaca tacttcgggt atacatatca gtatatattc
2041 ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg
2101 atccacgcgg cgtttacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
2161 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
2221 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
2281 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
2341 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
2401 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
2461 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
2521 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
2581 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
2641 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
2701 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
2761 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
2821 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
2881 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
2941 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
3001 atgctgccaa cttagtcgac tacaggtcac taataccatc taagtagttg attcatagtg
3061 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
3121 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
3181 atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
3241 ctacgcgtac cggttagtaa tgagtttaaa cgggggaggc taactgaaac acggaaggag
3301 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
3361 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
3421 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
3481 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
3541 agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
3601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
3661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
3721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
3781 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
3841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
3901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
3961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattatc ctcgagctac
4021 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
4081 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
4141 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
4201 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
4261 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
4321 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
4381 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
4441 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
4501 tcctcatctc cgggcctttc gactctagac acgtgttgac aattaatcat cggcatagta
4561 tatcggcata gtataatacg acaaggtgag gaactaaacc atggtgagca agggcgagga
4621 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
4681 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
4741 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta
4801 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
4861 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
4921 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
4981 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
5041 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
5101 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
5161 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
5221 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
5281 cgccgggatc actctcggca tggacgagct gtacaaggcc aagcctttgt ctcaagaaga
5341 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
5401 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
5461 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
5521 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
5581 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
5641 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
5701 gtgggagggc taagcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
5761 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
5821 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
5881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
5941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
6001 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
6061 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
6121 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
6181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
6241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
6301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
6361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
6421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
6481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
6541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
6601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
6661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
6721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
6781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
6841 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
6901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
6961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
7021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
7081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
7141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
7201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
7261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
7321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
7381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
7441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
7501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
7561 tcagcttcgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
7621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
7681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
7741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
7801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
7861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
7921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
7981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
8041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
8101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
8161 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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