| 质粒名称: | pAAV-CAG-NES-iYTnC2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000190 |
| 产品用途: | Expresses the iYTnC2 green calcium indicator based on YFP and troponin from O. tau toadfish in the cytosol of mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Mach1
|
|
插入基因信息 |
|
| 插入基因名称: | iYTnC2 |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics
Doronin D.A., Barykina N.V., Subach O.M., Sotskov V.P., Plusnin V.V., Ivleva O.A., Isaakova E.A., Varizhuk A.M., Pozmogova G.E.E., Malyshev A.Y., Smirnov I.V., Piatkevich K.D., Anokhin K.V., Enikolopov G.N., Subach F.V.
BMC Biotechnol. 18, 1–15 (2018)
|
买家导航
pAAV-CAG-NES-iYTnC2载体质粒基本信息
pAAV-CAG-NES-iYTnC2质粒图谱载体图谱和pAAV-CAG-NES-iYTnC2载体序列质粒序列多克隆位点信息
pAAV-CAG-NES-iYTnC2质粒载体简介
pAAV-CAG-NES-iYTnC2质粒序列载体序列
LOCUS pAAV-CAG-NES-iYTnC2 5749 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5749)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..5749
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
CDS 3652..3678
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 3688..4623
/codon_start=1
/label=iYTnC2
/translation="MVSKGEELFTGVVPILVELVGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERAIFFEDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLVRSEEELSESFRTFDKD
GDGFIDREEFGGIIRLTGEQLTDEDPDEIFGDSDTDKNGRIDFDEFLKMVENVQMFNSH
NVYIMADKQRNGIKAHFKVCHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHRSAL
SKDPNEKRDHMVLLESVTAVGITLGMDELYK"
misc_feature 4651..5239
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5122..5133)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5282..5403
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccgaca ccatgcttca acttcctcct
3661 cttgaacgtc ttactctttc gagatctatg gtgagcaagg gcgaggagct gttcaccggg
3721 gtggtgccca tcctggtcga gctggtcggc gacgtaaacg gccacaagtt cagcgtgtcc
3781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
3841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg cctgcagtgc
3901 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
3961 ggctacgtcc aggagcgtgc catcttcttc gaggacgacg gcaactacaa gacccgcgcc
4021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
4081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acctggtccg cagcgaagaa
4141 gagctatcag agtcctttcg cacctttgac aaggatgggg atggtttcat cgacagggag
4201 gaatttggag gcatcatccg ccttactgga gaacagctca cagatgagga tcccgatgag
4261 atttttggag actcagacac ggacaaaaat ggaaggattg actttgatga gttcctgaag
4321 atggtggaga atgtccagat gttcaacagc cacaacgtct atatcatggc cgacaagcag
4381 aggaacggca tcaaggccca cttcaaggtc tgccacaaca tcgaggacgg cagcgtgcag
4441 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
4501 aaccactacc tgagccaccg gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
4561 atggtcctgc tggagtccgt gaccgccgtc gggatcactc tcggcatgga cgagctgtac
4621 aagtaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
4681 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
4741 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
4801 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
4861 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
4921 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
4981 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
5041 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
5101 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
5161 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
5221 ctttgggccg cctccccgca tcgataccgt cgacccgggc ggccgcttcg agcagacatg
5281 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
5341 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
5401 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
5461 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca
5521 tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat
5581 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
5641 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat
5701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct
//
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