| 质粒名称: | AbVec2.0-IGHG2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 99576 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 空载体大小: | 5729bp |
| 完整质粒大小: | 5729bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | GCTTCGTTAGAACGCGGCTAC |
|
文献引用方法 |
|
| 材料和方法部分: |
AbVec2.0-IGHG2 was a gift from Hedda Wardemann (Addgene plasmid # 99576 ; http://n2t.net/addgene:99576 ; RRID:Addgene_99576)
|
买家导航
AbVec2.0-IGHG2载体质粒基本信息
AbVec2.0-IGHG2质粒图谱载体图谱和AbVec2.0-IGHG2载体序列质粒序列多克隆位点信息
AbVec2.0-IGHG2质粒载体简介
AbVec2.0-IGHG2质粒序列载体序列
LOCUS AbVec2.0-IGHG2 5729 bp DNA circular SYN 25-AUG-2024
DEFINITION Expression of secretory immunoglobulin heavy chains in mammalian
cells, human IgG2 isotype.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5729)
TITLE Immunoglobulin expression vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5729)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..5729
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 98..119
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 134..164
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 172..188
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 177..199
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 196..212
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 196..212
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
enhancer 241..620
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 621..824
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 775..795
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 821..845
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 1055..1073
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1055..1072
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 1086..1105
/label=pMT2-F
/note="Synthetic intron, forward primer"
regulatory 2256..2265
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
polyA_signal 2269..2403
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2306..2325)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2360..2379
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 2472..2829
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2472..2663
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2495..2515)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2680..2815
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(2849..2866)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2849..2865)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2858..2880)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3078..3533
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3165..3184)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3375..3396
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3550..3569)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3669..3691
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3729..3747)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3815..3919
/gene="bla"
/label=AmpR promoter
CDS 3920..4780
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4138..4157)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4951..5539
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5440..5459
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5693..5710
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atccaactgg cacgacaggt
61 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
121 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
181 gataacaatt tcacacagga aacagctatg acatgattac gaattaattc gagctcgccc
241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
841 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
901 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
961 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
1021 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
1141 cgattgaatt ccaccatggg atggtcatgt atcatccttt ttctagtagc aactgcaacc
1201 ggttgactaa ctagctggtc gaccaagggc ccaagcgtct tccccctggc gccctgctcc
1261 aggagcacct ccgagagcac agcggccctg ggctgcctgg tcaaggacta cttccccgag
1321 ccggtgacgg tgtcgtggaa ctcaggcgct ctgaccagcg gcgtgcacac cttcccagct
1381 gtcctacagt cctcaggact ctactccctc agcagcgtgg tgaccgtgcc ctccagcaac
1441 ttcggcaccc agacctacac ctgcaacgta gatcacaagc ccagcaacac caaggtggac
1501 aagacagttg agcgcaaatg ttgtgtcgag tgcccaccgt gcccagcacc acctgtggca
1561 ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc
1621 cctgaggtca cgtgcgtggt ggtggacgtg agccacgaag accccgaggt ccagttcaac
1681 tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccacggga ggagcagttc
1741 aacagcacgt tccgtgtggt cagcgtcctc accgttgtgc accaggactg gctgaacggc
1801 aaggagtaca agtgcaaggt ctccaacaaa ggcctcccag cccccatcga gaaaaccatc
1861 tccaaaacca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggag
1921 gagatgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta ccccagcgac
1981 atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacacctccc
2041 atgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga caagagcagg
2101 tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac
2161 acgcagaaga gcctctccct gtctccgggt aaatgagtgc cacggccggc aagcccccgc
2221 tccccaggct ctcggggtcg cgcgaggaaa gcttggccgc catggcccaa cttgtttatt
2281 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
2341 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
2401 atcgggaatt aattcggcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
2461 ttaggtacct tctgaggcgg aaagaaccat ctgtggaatg tgtgtcagtt agggtgtgga
2521 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
2581 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2641 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
2701 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
2761 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
2821 ttttgcaaaa agctgttaac agcttggcac tggccgtcgt tttacaacgt cgtgactggg
2881 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagttggc
2941 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
3001 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
3061 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
3121 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
3181 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
3241 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
3301 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
3361 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
3421 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
3481 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
3541 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
3601 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
3661 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
3721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
3781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
3841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
3901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
3961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
4021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
4081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
4141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
4201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
4261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
4321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
4381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
4441 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
4501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
4561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
4621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
4681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
4741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
4801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
4921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
5041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
5101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
5401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
5461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
5521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
5641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
5701 agtgagcgag gaagcggaag agcgcccaa
//
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