pUAST

LOCUS       pUAST                8897 bp DNA     circular SYN 12-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          124..189
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             319..339
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    611..745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(1250..1321,1716..1989,2064..2718,2780..3095,3316..3447,
                     3518..4132)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    4910..5495
                     /label=P element 5' end
     rep_origin      complement(5730..6318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6489..7349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7350..7454)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8256..8488
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    8533..8627
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        8646..8884
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 gaattcgtta acagatctgc ggccgcggct cgagggtacc tctagaggat ctttgtgaag
       61 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
      121 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
      181 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
      241 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
      301 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
      361 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
      421 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
      481 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
      541 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      601 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      661 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      721 caatgtatct tatcatgtct ggatcggatc cactagaagg ccttagtatg tatgtaagtt
      781 aataaaaccc ttttttggag aatgtagatt taaaaaaaca tatttttttt ttatttttta
      841 ctgcactgga catcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta
      901 tttgaagtac caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca
      961 gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc
     1021 gctatctctt tcgccaccgt ttgtagcgtt acctagcgtc aatgtccgcc ttcagttgca
     1081 ctttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag
     1141 tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg
     1201 tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga
     1261 ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa
     1321 tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca
     1381 acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaatcgca
     1441 caaacaaata gtgacacgaa acagattatt ctggtagctg tgctcgctat ataagacaat
     1501 ttttaagatc atatcatgat caagacatct aaaggcattc attttcgact acattctttt
     1561 ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata
     1621 aaagtataaa cctacttcgt aggatacttc gttttgttcg gggttagatg agcataacgc
     1681 ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg gcttcgcaga
     1741 gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctcctg ccacccagtc
     1801 cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc tatgcctggc
     1861 acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg cagctggtca
     1921 accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg aaacatttgc
     1981 tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt
     2041 ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt
     2101 gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc
     2161 gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc
     2221 caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct
     2281 aacggccagg gaacacctga ttttccaggc catggtgcgg atgccacgac atctgaccta
     2341 tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca
     2401 gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg
     2461 tctggcattc gcctccgagg cactaaccga tccgccgctt ctgatctgcg atgagcccac
     2521 ctccggactg gactcattta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca
     2581 gaagggcaag accgtcatcc tgaccattca tcagccgtct tccgagctgt ttgagctctt
     2641 tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc
     2701 cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag cattacatta
     2761 catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat ccggcggact
     2821 tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt gatcggatcg
     2881 ccaagatatg cgacaatttt gctattagca aagtagcccg ggatatggag cagttgttgg
     2941 ccaccaaaaa tttggagaag ccactggagc agccggagaa tgggtacacc tacaaggcca
     3001 cctggttcat gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac
     3061 cactcctcgt aaaagtgcga cttattcaga caacggtgag tggttccagt ggaaacaaat
     3121 gatataacgc ttacaattct tggaaacaaa ttcgctagat tttagttaga attgcctgat
     3181 tccacaccct tcttagtttt tttcaatgag atgtatagtt tatagttttg cagaaaataa
     3241 ataaatttca tttaactcgc gaacatgttg aagatatgaa tattaatgag atgcgagtaa
     3301 cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc
     3361 acgcaagtgg gcgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc
     3421 tttcaaaacg tctttgccac gataaatgta agtcttgttt agaatacatt tgcatattaa
     3481 taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt
     3541 ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac
     3601 gattgccgaa ttaccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc
     3661 gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct
     3721 ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc
     3781 gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt
     3841 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
     3901 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
     3961 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
     4021 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt
     4081 ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata
     4141 tatccgaaat aactgcttgt tttttttttt accattatta ccatcgtgtt tactgtttat
     4201 tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat
     4261 agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa
     4321 ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac
     4381 accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc
     4441 tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac
     4501 attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg
     4561 ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat
     4621 gtacaattta tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta
     4681 atttaatatt atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta
     4741 caatttgaac tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa
     4801 tggtgggcat aatagtgttg tttatatata tcaaaaataa caactataat aataagaata
     4861 catttaattt agaaaatgct tggatttcac tggaactaga attaattcgg ctgctgctct
     4921 aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt attttcatta
     4981 aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga ttttttttac
     5041 tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta tcttttctgg
     5101 ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc ttttaaatgt
     5161 ctgacctttt gcaggtgcag ccttccactg cgaatcatta aagtgggtat cacaaatttg
     5221 ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct taatagcaca
     5281 cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt tgcaatattt
     5341 catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa
     5401 cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg taagcttcgg
     5461 ctatcgacgg gaccacctta tgttatttca tcatgggcca gacccacgta gtccagcggc
     5521 agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact ggggcacgtg
     5581 gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg gttaatcagc
     5641 agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag gtatactggc
     5701 accgagcccg agttcaagaa gaaggcgttt ttccataggc tccgcccccc tgacgagcat
     5761 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5821 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5881 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     5941 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6001 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6061 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6121 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     6181 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6241 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6301 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6361 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6421 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6481 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6541 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6601 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6661 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6721 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6781 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6841 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6901 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6961 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7021 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7081 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     7141 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7201 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7261 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7321 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7381 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7441 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     7501 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     7561 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     7621 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7681 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7741 tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca
     7801 aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg
     7861 gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat
     7921 atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta
     7981 agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata
     8041 cttgtattct ataggtcata tcttgttttt attggcacaa atataattac attagctttt
     8101 tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa acaagcagtt
     8161 atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc
     8221 gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca taaggtggtc
     8281 ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg aaaggaatag
     8341 tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga ttaaccctta
     8401 gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta tttttaaagt
     8461 tttattttta ataatttgcg agtacgcaaa gcttctgcat ctctccggat ccaagcttgc
     8521 atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     8581 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactctagc
     8641 gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
     8701 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
     8761 aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc agcaaccaag
     8821 taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca agaagagaac
     8881 tctgaatagg gaattgg
//