pAAV-CAG-DIO-ElectraON-Kv2.1

LOCUS       pAAV-CAG-DIO-ElectraON-Kv2.1                2401 bp DNA     linear   SYN 27-FEB-2024
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..2401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..44
                     /label=WPRE-R
     protein_bind    complement(68..101)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(172..205)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     misc_feature    225..230
                     /label=Kozak sequence
     CDS             231..917
                     /codon_start=1
                     /label=AcePR
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYAKYIDWVLTTPLLLLNLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILFVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSY"
     regulatory      231..234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             921..1589
                     /codon_start=1
                     /label=mBaoJin2(codon optimized)
                     /translation="STPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPM
                     SWAALGTSFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGSIHAKHQHFMKN
                     GTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPHIPRDDGVECPVTLLYPLLSDKSKY
                     VEAYQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETLEAHLKGMDELY
                     K"
     CDS             1590..1649
                     /codon_start=1
                     /label=KGC
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             1650..1670
                     /codon_start=1
                     /label=ER2
                     /translation="FCYENEV"
     CDS             1671..1865
                     /codon_start=1
                     /label=Kv2.1
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     primer_bind     complement(1846..1889)
                     /label=0130-FLEX-BSRGI-R
     primer_bind     complement(1846..1867)
                     /label=Kv2.1-Rv-ER2
     protein_bind    1893..1926
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    1989..2022
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
ORIGIN
        1 caaattttgt aatccagagg ttgattatcg ataagcttga tatcgaattc gaacctcttc
       61 gagggacata acttcgtata atgtatgcta tacgaagtta tgggtcgatg gtgagatctg
      121 gactagaggg tcgatggtga tgcttggatc aaaataggaa gaatcccaat gataacttcg
      181 tataaagtat cctatacgaa gttatactag aattcggtac ctaagccacc atggctgacg
      241 tggaaaccga gaccggcatg attgcacagt ggattgtctt tgctattatg gctgctgctg
      301 ctattgcttt tggagtggct gtgcactttc ggccttcaga gctgaagagc gcatactata
      361 tcaacattgc catctgcact atcgccgcta ccgcttacta tgcaatggcc gtgaactacc
      421 aggacctgac aatgaatggt gaaaggcagg tggtctacgc aaagtatatt gactgggtgc
      481 tgaccacacc actgctcctg ctcaacctca tcgtcatgac caagatgggc ggagtgatga
      541 tttcttgggt catcggcgca gacattttca tgatcgtgtt tggtattctg ggcgccttcg
      601 aggatgaaca caagttcaaa tgggtgtact ttatcgctgg atgtgtgatg caggcagtcc
      661 tgacatacgg gatgtataac gccacttgga aagacgatct gaagaaaagc cccgagtacc
      721 atagctccta tgtcagtctg ctcgtcttcc tgtcaatcct cttcgtgttt tatcctgtcg
      781 tgtgggcttt cgggtctggt agtggcgtgc tgtccgtcga caatgaggcc attctcatgg
      841 gaatcctgga tgtgctcgct aagccactgt ttggaatggg gtgcctcatt gcccatgaga
      901 ctatcttcaa gtcctacgcc agtactccct tcaagtttca gctcaaaggg accatcaatg
      961 ggaagagctt cactgttgag ggagagggcg aaggaaactc tcatgagggt tctcacaagg
     1021 ggaaatatgt ctgcacatct ggaaagcttc ccatgtcatg ggcagccttg ggcacaagtt
     1081 tcggctatgg catgaagtac tacacgaaat acccatccgg tctgaagaac tggtttcgag
     1141 aggtgatgcc tggtggcttt acctatgaca ggcatatcca gtacaaggga gatggatcca
     1201 ttcatgcgaa gcaccagcac ttcatgaaga atgggacgta ccacaacatt gtggagttta
     1261 ccggtcaaga cttcaaggag aatagcccgg tgttgacagg cgatatgaat gtgagccttc
     1321 ccaacgaagt ccctcacata cctagagatg atggcgttga gtgtccagtc acactcctgt
     1381 acccactgct gagcgacaag tccaagtatg tggaagccta tcagtacacc atctgcaaac
     1441 cgctgcacaa tcaaccagct cctgatgtgc cctatcactg gattcggaaa cagtatactc
     1501 agagcaaaga tgacgcagaa gaacgcgacc atatctgtca gtcagaaacc ctggaagctc
     1561 atctgaaagg gatggacgag ctctacaaaa agagcaggat caccagcgag ggcgagtaca
     1621 tccccctgga ccagatcgac atcaacgtgt tctgctacga gaacgaggtg cagagccagc
     1681 ctatcctgaa caccaaggag atggcccctc agagcaagcc tcctgaggag ctggagatga
     1741 gcagcatgcc tagccctgtg gcccctctgc ctgccaggac cgagggcgtg atcgacatga
     1801 ggagcatgag cagcatcgac agcttcatca gctgcgccac cgacttccct gaggccacca
     1861 ggttctaatg tacaagtaag gggatcccta gtataacttc gtatagcata cattatacga
     1921 agttatgggt cgatggtgat gcttggcaat tcgggtcgat ggtgaagcat tggtcttcct
     1981 attttgatat aacttcgtat aggatacttt atacgaagtt atggggatcc aattctttgc
     2041 caaaatgatg agacagcaca ataaccagca cgttgcccag gagctgtagg aaaaagaaga
     2101 aggcatgaac atggttagca gaggctctag agccgccggt cacacgccag aagccgaacc
     2161 ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg cggacagccc cgagccgctc
     2221 cccccgcaca aagggccctc ccggagcccc tcaaggcttt cacgcagcca cagaaaagaa
     2281 acaagccgtc attaaaccaa gcgctaatta cagcccggag gagaagggcc gtcccgcccg
     2341 ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg gcggggcccc aaaggcggcg
     2401 c
//