| 质粒名称: | CL20_mEGFP_ETV6_NTRK3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 205817 |
| 存储实验室: | Richard Kriwacki |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 9024 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ETV6_NTRK3 |
| 物种来源: | H. sapiens (human) |
| 标签/融合蛋白: | mEGFP (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | CL20_mEGFP_ETV6_NTRK3 was a gift from Richard Kriwacki (Addgene plasmid # 205817 ; http://n2t.net/addgene:205817 ; RRID:Addgene_205817) |
| 参考文献部分: | Defining the condensate landscape of fusion oncoproteins. Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG, Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni N, Kriwacki RW. Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2. 10.1038/s41467-023-41655-2 PubMed 37770423 |
买家导航
CL20_mEGFP_ETV6_NTRK3载体质粒基本信息
CL20_mEGFP_ETV6_NTRK3质粒图谱载体图谱和CL20_mEGFP_ETV6_NTRK3载体序列质粒序列多克隆位点信息
CL20_mEGFP_ETV6_NTRK3质粒载体简介
CL20_mEGFP_ETV6_NTRK3质粒序列载体序列
LOCUS CL20_mEGFP_ETV6_NTRK3 9024 bp DNA circular SYN 14-APR-2025
DEFINITION Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived
sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9024)
AUTHORS Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q,
Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR,
McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni
N, Kriwacki RW
TITLE Defining the condensate landscape of fusion oncoproteins.
JOURNAL Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
PUBMED 37770423
REFERENCE 2 (bases 1 to 9024)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: "
10.1038/s41467-023-41655-2"
COMMENT Sequence Label: CL20_mEGFP_ETV6_NTRK3
FEATURES Location/Qualifiers
source 1..9024
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 220..239
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 473..490
/label=L4440
/note="L4440 vector, forward primer"
polyA_signal 558..692
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(582..601)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 636..655
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter complement(698..893)
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 712..847
/label=SV40 ori
/note="SV40 origin of replication"
protein_bind 952..973
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(988..993,994..1011,1012..1018)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1026..1042
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1031..1053
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 1050..1066
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1050..1066
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 1409..1428
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
polyA_signal 1427..1482
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind 1528..1547
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
CDS complement(join(3785..4495,4496..4498,4499..4501))
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3827..3848)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 4176..4195
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4435..4456
/label=EGFP-N
/note="EGFP, reverse primer"
regulatory 4498..4507
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter complement(4583..4786)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(4612..4632)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 4787..5090
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
CDS complement(5347..5391)
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 5576..5809
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 6169..6286
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 6778..6903
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 6950..7207
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
enhancer 7209..7588
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 7619..7723
/gene="bla"
/label=AmpR promoter
CDS join(7724..7792,7793..8584)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7942..7961)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin join(8755..9024,1..319)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagc
1801 ccagaatatc caggtaaatc ggcgtagcct tcccaagagc gtgaagaatt ttatagattt
1861 ccttaatatt cagacgctgt tgcggctcac gctgccagca ccccagcatt acgtcataaa
1921 cttcttttgg gcacacgcgt gggcgttcaa gaacacgccc ttgggtaatg cattcgatca
1981 cctcggtgtt tgacagctga aaccaaggtt gctttccata cgtaaagatt tcccacagga
2041 taacaccaaa gctccaaacg tcgctctccg tggtgaattt gcggtacatg atggattccg
2101 ggggcatcca gcggataggt aacattgtgt gaccacctac ctcgcaccaa atgcaaaaat
2161 cgttccctga agggttgaac agacggtaat agtctgttga gtaaacgtca cgactcatac
2221 cgaagtcccc gatcttaact aacaggtttg caccaacaag acagttgcga gtagcaagat
2281 cacgatgcac gaagtgctga gaggcaaggt ataccattcc cgacgcaatc tgcgacgcga
2341 tgtgaagcat ttgggacagg cccagttcac ctttagcctg acgtggttga ccgtccacaa
2401 gaatcatcgc gtctggaccg tgcgcacgca ggaatttatt caggtctcca tgcttcatgt
2461 attcgaagac catgatcaac ggatcaccat ctccacaaac cccgtagaat ttcacaatgt
2521 gttcatgctg caagttggtt aataattccg cttcacgttg gaagtcttta cgggccgcca
2581 gggttggatc tttaagcgct tttacggcta ccaacatttt gtcttttgtc ggacttaaat
2641 tgtagcactc tgccagaaac accttcccaa atgcgccttc ccctaattcg cgtttcagta
2701 cgatgtcacg acgcttaata tgttgcacat ctgcgatgcg accgattggc atggcatgtt
2761 cctcgggggg gcttaccgaa accatgatat ggttcatata agcaaggtcc tcacgatgtg
2821 acaagttaat tggctttcct tcacggtgaa gtccatcctc tgacaagcga ctttgcttga
2881 agtctacgga atggcgcggg ttaaggatca atgggtgcat aataggagac ggcataagtt
2941 ggattacacg cgtactttct tggcgtggac tgcttggctt ggggtggctc tctgacgagg
3001 cagggcaatg gttgttttcc atgggagaaa cgctcaacgg ataactttct tggtgattgt
3061 tttcttgatg cggacggggg ccttgcgcgc gttcggccgg gctcagacga cggatcatgt
3121 tgtctaaggg actacgcagt ggacgttgct ccgggtcggg tgaaggacgg tggtttgtcg
3181 tgattgggct acggctacgg tgaagcagtt cgatggtagg tggattgtga tgtacgttgt
3241 caacggaagg gcgtggggtg cgttgtacgc aattgtcctc ttcgtgattc tggtgtaaaa
3301 ttacctctgg ttgtgtgtgg atggagttac cgggatgaaa gaatggagaa aacagaatgc
3361 ggggcttacg ttgtttcaaa atatgctgca gcagctcata taagacatct ccactatgcg
3421 gactacgata acgaaagtct tctttagtta acaataacaa tgcttttccg ttcatctcaa
3481 aggtgttaga gtcgatgggg cgcaaggaaa attcgttctc cgcccatttc agccattgtg
3541 cgacatcgtc gcgagaccag taaatcggtt gtaaacgcaa gtgcgcgggc agacgaatgg
3601 aatcttcttc catgcgcaat gcgcgcggaa ccggaacgtg taagggggtc gaggacgcat
3661 aagaagggac aggactttct ggaggcgtat aagaaatgcg ttcttgctta atggaacact
3721 gtgcaggagt ctcggacatt ccccgggaag cggccgcact tcccccggag cctccgcttc
3781 cgcccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
3841 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt
3901 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
3961 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
4021 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
4081 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
4141 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
4201 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
4261 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
4321 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
4381 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
4441 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
4501 tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt cggttcacta
4561 aacgagcccg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac
4621 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
4681 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
4741 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
4801 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
4861 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
4921 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
4981 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
5041 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
5101 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gcacgcgttc
5161 gaagcttgag ctcggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
5221 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
5281 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
5341 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
5401 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
5461 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
5521 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
5581 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
5641 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
5701 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
5761 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
5821 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
5881 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
5941 ttatggtgtt ttactaaact tttccatgtt ctaatcctca tcctgtctac ttgccacaca
6001 atcatcacct gccatctgtt ttccataatc cctaatgatc tttgcttttc ttcttggcac
6061 tacttttatg tcactattat cttgtattac tactgcccct tcacctttcc agaggagctt
6121 tgctggtcct ttccaaagtg gatttctgct gtccctgtaa taaacccgaa aattttgaat
6181 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
6241 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
6301 tgccatttgt actgctgtct taagatgttc agcctgatct cttacctgtc ctataatttt
6361 ctttaattct ttattcatag attctactac tccttgactt tggggattgt agggaatttc
6421 caggtctgaa gatcctgctg cttgctgtgc ttttttctta cttttgtttt gctcttcctc
6481 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
6541 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
6601 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag tattaactgc
6661 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
6721 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
6781 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
6841 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
6901 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
6961 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
7021 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
7081 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca
7141 aaaagcagct gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc
7201 acgcctccca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
7261 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
7321 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
7381 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
7441 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
7501 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
7561 tattaataac tagtcaataa tcaatgtcga cccaggtggc acttttcggg gaaatgtgcg
7621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
7681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
7741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
7801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
7861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
7921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
7981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
8041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
8101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
8161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
8221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
8281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
8341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
8401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
8461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
8521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
8581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
8641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
8701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
8761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
8821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
8881 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
8941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
9001 tggcgataag tcgtgtctta ccgg
//
