| 质粒名称: | pCMV-hA3A-BE3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 113410 |
| 存储实验室: | Jia Chen |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 空载体大小: | 4399 bp |
| 完整质粒大小: | 8442 bp |
| 载体类型: | Mammalian Expression, CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | hA3A-BE3 / pCMV-hAPOBEC3A-XTEN-D10A-SGGS-UGI-SGGS-NLS |
| 物种来源: | H. sapiens (human); S. pyogenes and Bacteriophage PBS2 |
| 插入大小: | 5043bp |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CMV |
| 3’测序引物 | BGH poly reverse |
|
文献引用方法 |
|
| 材料和方法部分: |
pCMV-hA3A-BE3 was a gift from Jia Chen (Addgene plasmid # 113410 ; http://n2t.net/addgene:113410 ; RRID:Addgene_113410)
|
| 参考文献部分: | Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L. Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198. 10.1038/nbt.4198 PubMed 30125268 |
买家导航
pCMV-hA3A-BE3载体质粒基本信息
pCMV-hA3A-BE3质粒图谱载体图谱和pCMV-hA3A-BE3载体序列质粒序列多克隆位点信息
pCMV-hA3A-BE3质粒载体简介
pCMV-hA3A-BE3质粒序列载体序列
LOCUS pCMV-hA3A-BE3 8442 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses hA3A-BE3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-hA3A-BE3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8442)
AUTHORS Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J,
Yang L
TITLE Efficient base editing in methylated regions with a human
APOBEC3A-Cas9 fusion.
JOURNAL Nat Biotechnol., 2018
REFERENCE 2 (bases 1 to 8442)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8442
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 124..503
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 504..707
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 658..678
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 749..768
/label=T7
/note="T7 promoter, forward primer"
promoter 749..767
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 775..784
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 781..1377
/codon_start=1
/gene="human APOBEC3A"
/gene_synonym="A3A"
/product="human cytidine deaminase (C to U editing enzyme)
that efficiently edits methylated cytosines (Wang et al.,
2018)"
/label=APOBEC3A
/note="also known as hA3A"
/translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
CDS 1426..5526
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5539..5787
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5800..5820
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5829..5846
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5869..5886)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5875..6099
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6170..6186)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6170..6186)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6183..6205)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6194..6210
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6218..6248)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6263..6284
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6401..6418)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6572..7160)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6652..6671)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7331..8191)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7954..7973
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8192..8296)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8375..8394)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
61 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
121 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
181 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
241 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
301 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
361 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
421 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
481 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
541 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
601 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
661 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
721 gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc
781 atggaagcca gcccagcatc cgggcccaga cacttgatgg atccacacat attcacttcc
841 aactttaaca atggcattgg aaggcataag acctacctgt gctacgaagt ggagcgcctg
901 gacaatggca cctcggtcaa gatggaccag cacaggggct ttctacacaa ccaggctaag
961 aatcttctct gtggctttta cggccgccat gcggagctgc gcttcttgga cctggttcct
1021 tctttgcagt tggacccggc ccagatctac agggtcactt ggttcatctc ctggagcccc
1081 tgcttctcct ggggctgtgc cggggaagtg cgtgcgttcc ttcaggagaa cacacacgtg
1141 agactgcgta tcttcgctgc ccgcatctat gattacgacc ccctatataa ggaggcactg
1201 caaatgctgc gggatgctgg ggcccaagtc tccatcatga cctacgatga atttaagcac
1261 tgctgggaca cctttgtgga ccaccaggga tgtcccttcc agccctggga tggactagat
1321 gagcacagcc aagccctgag tgggaggctg cgggccattc tccagaatca gggaaacagc
1381 ggcagcgaga ctcccgggac ctcagagtcc gccacacccg aaagtgataa aaagtattct
1441 attggtttag ccatcggcac taattccgtt ggatgggctg tcataaccga tgaatacaaa
1501 gtaccttcaa agaaatttaa ggtgttgggg aacacagacc gtcattcgat taaaaagaat
1561 cttatcggtg ccctcctatt cgatagtggc gaaacggcag aggcgactcg cctgaaacga
1621 accgctcgga gaaggtatac acgtcgcaag aaccgaatat gttacttaca agaaattttt
1681 agcaatgaga tggccaaagt tgacgattct ttctttcacc gtttggaaga gtccttcctt
1741 gtcgaagagg acaagaaaca tgaacggcac cccatctttg gaaacatagt agatgaggtg
1801 gcatatcatg aaaagtaccc aacgatttat cacctcagaa aaaagctagt tgactcaact
1861 gataaagcgg acctgaggtt aatctacttg gctcttgccc atatgataaa gttccgtggg
1921 cactttctca ttgagggtga tctaaatccg gacaactcgg atgtcgacaa actgttcatc
1981 cagttagtac aaacctataa tcagttgttt gaagagaacc ctataaatgc aagtggcgtg
2041 gatgcgaagg ctattcttag cgcccgcctc tctaaatccc gacggctaga aaacctgatc
2101 gcacaattac ccggagagaa gaaaaatggg ttgttcggta accttatagc gctctcacta
2161 ggcctgacac caaattttaa gtcgaacttc gacttagctg aagatgccaa attgcagctt
2221 agtaaggaca cgtacgatga cgatctcgac aatctactgg cacaaattgg agatcagtat
2281 gcggacttat ttttggctgc caaaaacctt agcgatgcaa tcctcctatc tgacatactg
2341 agagttaata ctgagattac caaggcgccg ttatccgctt caatgatcaa aaggtacgat
2401 gaacatcacc aagacttgac acttctcaag gccctagtcc gtcagcaact gcctgagaaa
2461 tataaggaaa tattctttga tcagtcgaaa aacgggtacg caggttatat tgacggcgga
2521 gcgagtcaag aggaattcta caagtttatc aaacccatat tagagaagat ggatgggacg
2581 gaagagttgc ttgtaaaact caatcgcgaa gatctactgc gaaagcagcg gactttcgac
2641 aacggtagca ttccacatca aatccactta ggcgaattgc atgctatact tagaaggcag
2701 gaggattttt atccgttcct caaagacaat cgtgaaaaga ttgagaaaat cctaaccttt
2761 cgcatacctt actatgtggg acccctggcc cgagggaact ctcggttcgc atggatgaca
2821 agaaagtccg aagaaacgat tactccatgg aattttgagg aagttgtcga taaaggtgcg
2881 tcagctcaat cgttcatcga gaggatgacc aactttgaca agaatttacc gaacgaaaaa
2941 gtattgccta agcacagttt actttacgag tatttcacag tgtacaatga actcacgaaa
3001 gttaagtatg tcactgaggg catgcgtaaa cccgcctttc taagcggaga acagaagaaa
3061 gcaatagtag atctgttatt caagaccaac cgcaaagtga cagttaagca attgaaagag
3121 gactacttta agaaaattga atgcttcgat tctgtcgaga tctccggggt agaagatcga
3181 tttaatgcgt cacttggtac gtatcatgac ctcctaaaga taattaaaga taaggacttc
3241 ctggataacg aagagaatga agatatctta gaagatatag tgttgactct taccctcttt
3301 gaagatcggg aaatgattga ggaaagacta aaaacatacg ctcacctgtt cgacgataag
3361 gttatgaaac agttaaagag gcgtcgctat acgggctggg gacgattgtc gcggaaactt
3421 atcaacggga taagagacaa gcaaagtggt aaaactattc tcgattttct aaagagcgac
3481 ggcttcgcca ataggaactt tatgcagctg atccatgatg actctttaac cttcaaagag
3541 gatatacaaa aggcacaggt ttccggacaa ggggactcat tgcacgaaca tattgcgaat
3601 cttgctggtt cgccagccat caaaaagggc atactccaga cagtcaaagt agtggatgag
3661 ctagttaagg tcatgggacg tcacaaaccg gaaaacattg taatcgagat ggcacgcgaa
3721 aatcaaacga ctcagaaggg gcaaaaaaac agtcgagagc ggatgaagag aatagaagag
3781 ggtattaaag aactgggcag ccagatctta aaggagcatc ctgtggaaaa tacccaattg
3841 cagaacgaga aactttacct ctattaccta caaaatggaa gggacatgta tgttgatcag
3901 gaactggaca taaaccgttt atctgattac gacgtcgatc acattgtacc ccaatccttt
3961 ttgaaggacg attcaatcga caataaagtg cttacacgct cggataagaa ccgagggaaa
4021 agtgacaatg ttccaagcga ggaagtcgta aagaaaatga agaactattg gcggcagctc
4081 ctaaatgcga aactgataac gcaaagaaag ttcgataact taactaaagc tgagaggggt
4141 ggcttgtctg aacttgacaa ggccggattt attaaacgtc agctcgtgga aacccgccaa
4201 atcacaaagc atgttgcaca gatactagat tcccgaatga atacgaaata cgacgagaac
4261 gataagctga ttcgggaagt caaagtaatc actttaaagt caaaattggt gtcggacttc
4321 agaaaggatt ttcaattcta taaagttagg gagataaata actaccacca tgcgcacgac
4381 gcttatctta atgccgtcgt agggaccgca ctcattaaga aatacccgaa gctagaaagt
4441 gagtttgtgt atggtgatta caaagtttat gacgtccgta agatgatcgc gaaaagcgaa
4501 caggagatag gcaaggctac agccaaatac ttcttttatt ctaacattat gaatttcttt
4561 aagacggaaa tcactctggc aaacggagag atacgcaaac gacctttaat tgaaaccaat
4621 ggggagacag gtgaaatcgt atgggataag ggccgggact tcgcgacggt gagaaaagtt
4681 ttgtccatgc cccaagtcaa catagtaaag aaaactgagg tgcagaccgg agggttttca
4741 aaggaatcga ttcttccaaa aaggaatagt gataagctca tcgctcgtaa aaaggactgg
4801 gacccgaaaa agtacggtgg cttcgatagc cctacagttg cctattctgt cctagtagtg
4861 gcaaaagttg agaagggaaa atccaagaaa ctgaagtcag tcaaagaatt attggggata
4921 acgattatgg agcgctcgtc ttttgaaaag aaccccatcg acttccttga ggcgaaaggt
4981 tacaaggaag taaaaaagga tctcataatt aaactaccaa agtatagtct gtttgagtta
5041 gaaaatggcc gaaaacggat gttggctagc gccggagagc ttcaaaaggg gaacgaactc
5101 gcactaccgt ctaaatacgt gaatttcctg tatttagcgt cccattacga gaagttgaaa
5161 ggttcacctg aagataacga acagaagcaa ctttttgttg agcagcacaa acattatctc
5221 gacgaaatca tagagcaaat ttcggaattc agtaagagag tcatcctagc tgatgccaat
5281 ctggacaaag tattaagcgc atacaacaag cacagggata aacccatacg tgagcaggcg
5341 gaaaatatta tccatttgtt tactcttacc aacctcggcg ctccagccgc attcaagtat
5401 tttgacacaa cgatagatcg caaacgatac acttctacca aggaggtgct agacgcgaca
5461 ctgattcacc aatccatcac gggattatat gaaactcgga tagatttgtc acagcttggg
5521 ggtgactctg gtggttctac taatctgtca gatattattg aaaaggagac cggtaagcaa
5581 ctggttatcc aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac
5641 aagccggaaa gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc
5701 atgcttctga ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc
5761 aacggtgaga acaagattaa gatgctctct ggtggttctc ccaagaagaa gaggaaagtc
5821 taaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
5881 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
5941 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
6001 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
6061 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
6121 gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
6181 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
6241 agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
6301 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
6361 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
6421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
6481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
6541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
6601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
6661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
6721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
6781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
6841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
6901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
6961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
7021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
7081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
7141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
7201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
7261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
7321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
7381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
7441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
7501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
7561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
7621 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
7681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
7741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
7801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
7861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
7921 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
7981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
8041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
8101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
8161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
8221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
8281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
8341 atcgatctcc cgatccccta gggtcgactc tcagtacaat ctgctctgat gccgcatagt
8401 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tg
//
