pCDH-LIAS-GFP

LOCUS       pCDH-LIAS-GFP        8286 bp DNA     circular SYN 13-MAY-2026
DEFINITION  contig1 circular.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1946..2249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2250..2453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2504..3619
                     /label=LIAS
     CDS             3644..4360
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    4367..4954
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4838..4849)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5027..5260
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5332..5453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5493..5628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5661..5677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5685..5701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5709..5739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5754..5775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6063..6651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6822..7682)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7683..7787)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8261..8277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     2461 tgaaccgtca gattctagac cgctagcgct accggtcgcc accatgtctc tacgctgcgg
     2521 ggatgcagcc cgcaccctgg ggccccgggt atttgggaga tatttttgca gcccagtcag
     2581 accgttaagc tccttgccag ataaaaaaaa ggaactccta cagaatggac cagaccttca
     2641 agattttgta tctggtgatc ttgcagacag gagcacctgg gatgaatata aaggaaacct
     2701 aaaacgccag aaaggagaaa ggttaagact acctccatgg ctaaagacag agattcccat
     2761 ggggaaaaat tacaataaac tgaaaaatac tttgcggaat ttaaatctac atacagtatg
     2821 tgaggaagct cgatgtccca atattggaga gtgttgggga ggtggagaat atgccaccgc
     2881 cacagccacg atcatgttga tgggtgacac atgtacaaga ggttgcagat tttgttctgt
     2941 taagactgca agaaatcctc ctccactgga tgccagtgag ccctacaata ctgcaaaggc
     3001 aattgcagaa tggggtctgg attatgttgt cctgacatct gtggatcgag atgatatgcc
     3061 tgatggggga gctgaacaca ttgcaaagac cgtatcatat ttaaaggaaa ggaatccaaa
     3121 aatccttgtg gagtgtctta ctcctgattt tcgaggtgat ctcaaagcaa tagaaaaagt
     3181 tgctctgtca ggattagatg tgtatgcaca taatgtagaa acagtcccgg aattacagag
     3241 taaggttcgt gatcctcggg ccaattttga tcagtcccta cgtgtactga aacatgccaa
     3301 gaaggttcag cctgatgtta tttctaaaac atctataatg ttgggtttag gcgagaatga
     3361 tgagcaagta tatgcaacaa tgaaagcact tcgtgaggca gatgtagact gcttgacttt
     3421 aggacaatat atgcagccaa caaggcgtca ccttaaggtt gaagaatata ttactcctga
     3481 aaaattcaaa tactgggaaa aagtaggaaa tgaacttgga tttcattata ctgcaagtgg
     3541 ccctttggtg cgttcttcat ataaagcagg tgaatttttc ctgaaaaatc tagtggctaa
     3601 aagaaaaaca aaagacctcg gtggaggctc aggtggtggt agcgtgagca agggcgagga
     3661 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3721 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3781 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3841 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3901 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3961 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4021 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4081 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4141 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4201 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     4261 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4321 cgccgggatc actctcggca tggacgagct gtacaagtag ggatccaatc aacctctgga
     4381 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4441 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4501 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4561 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4621 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4681 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4741 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     4801 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4861 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca
     4921 gacgagtcgg atctcccttt ggccgcctcc ccgccggtac ctttaagacc aatgacttac
     4981 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5041 cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     5101 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5161 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5221 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     5281 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     5341 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5401 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5461 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5521 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5581 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     5641 gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6001 aacatgtgag caaaaggcca gcaaaaggcc aggaacctta aaaaggccgc gttgctggcg
     6061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6241 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7801 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7861 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7921 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7981 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     8041 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     8101 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8161 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8221 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     8281 aagctg
//